Department Images 152 - 209 (Dustin) Flashcards
What organ is this from?
What does the arrow point to?
Star?
1, 2, 3?
Ovary
Arrow: Peritoneal Epithelium (simple cuboidal)
Star: Graafian Follicle (although it’s labeled as a Tertiary Follicle, but looks much like a deeper Graafian. Gonna ask about this one)
1: Primary Follicles
2: Primordial Follicles
3: Secondary Follicle (probably)
(lemme know if you think one of these is wrong! if I remember correctly, the Hungarian way to classify these is slightly different than in English books, maybe more similar to German books)
Where is this?
What type of epithelium do you see?
What’s the layer beneath it?
What are the round things?
Ovary
Peritoneal Epithelium (simple cuboidal)
Layer beneath it = Tunica Albuginea
Round things = Primordial Follicles
What type of follicles are the 3 large ones in the bottom half of the image?
And the medium-sized one right in the middle?
And all the little ones at the top right?
3 large ones: Secondary Follicles
Medium sized in middle: Primary Follicles
Top ones: Primordial Follicles
What kind of follicle?
Graafian
But the image is labeled as Tertiary Follicle, going to ask about this later
What is this from?
What are the paler cells that make up most of the image?
What are the darker cells at the top?
Corpus Luteum
Paler cells = Granulosa Lutein Cells
Darker cells = Theca Lutein Cells
What tissue is this from?
Uterine Tube / Oviduct, Ampullary Part
Simple Columnar, some ciliated and some non-ciliated
In some slides, the mesosalpynx might be seen: double layer of peritoneum containing blood vessels
Don’t mix it up with seminal vesicle
What tissue is this?
What type of cells do you see in the epithelium?
Oviduct, Ampulla
See Simple Columnar Epithelium with:
Ciliated Cells
Non-ciliated (secretory) Cells
“Peg” or “Pin” Cells - dark, elongated nuclei protruding into the lumen, degenerating cells
What tissue is this from?
Uterus - Proliferative Phase (early in phase)
Has relatively less glands than secretory phase, thinner endometrium
What tissue is this?
Uterus - Secretory Phase
Has many “tortuous” glands, thick endometrium
What tissue is this from?
What layers do you see?
Fetal portion of the Placenta
At the lowest part of the image is the simple cuboidal amnion epithelium
Above that is the chorionic plate: thick connective tissue
At the top you see chorionic villi
What tissue is this from?
1? 2?
What’s the space between the structures?
Placenta
1: Chorionic Villi (free)
2: Anchoring Villus
Intervillous Space - fills with maternal blood
Note also the syncitiotrophoblasts, cytotrophoblasts and other important placental structures
What tissue is this?
What does the image show?
Placenta
Shows the anchoring villus connecting with the basal plate and decidual cells, with eosinophilic fibrinoid
(I think, anyway)
What’s the big pink blob?
Placental Septum with mainly decidual cells
What tissue is this?
What does this show?
Placenta
Shows the anchoring villus connecting with the basal plate and decidual cells, with eosinophilic fibrinoid
(another one)
What tissue is this?
Vagina
Note:
- Wide lamina propria mucosae has lots of CT with elastic fibers
- Vaginal wall has neither glands nor submucosa
- Esophagus easily distinguished from vagina bc esophagus has muscularis mucosae, tela submucosa, and glands
- Ureter has transitional epithelial lining and regular smooth muscle in its tunica muscularis
What tissue is this from?
Non-Lactating Breast
(Mamma Nonlactans)
What tissue is this?
Lactating Breast
Mamma Lactans
What tissue is this?
What glands are here?
Non-Lactating Breast
There are Areolar Glands of Montgomery here: apocrine secretion
Areolar glands or Glands of Montgomery are sebaceous glands in the areola surrounding the nipple. The glands make oily secretions (lipoid fluid) to keep the areola and the nipple lubricated and protected. Volatile compounds in these secretions may also serve as an olfactory stimulus for newborn appetite.
What tissue is this from?
What stain?
Be able to name the parts
Hypophysis
Chrome-Hematoxylin-Phloxin stain: helps differentiate between acidophils and basophils of the adenohypophysis (the bright red tissue to the right)
What tissue is this from?
What cells do you see?
Adenohypophysis - H-E Stain
Acidophils: bright red
Basophils: larger, basophilic cells
Chromophobes: poorly stained
What tissue is this?
What cells do you see?
Adenohypophysis
(with Chrome-Hematoxylin-Phloxin stain to show more of a difference between cells)
Acidophils: bright red
Basophils: larger blue cells
Chromophobes: poorly stained
What tissue is this?
Adenohypophysis
(Says “Kresazan” for stain, which either google translates as “road traffic,” or it’s some sort of altered Azan stain)
What tissue is this?
Adenohypophysis
(Says “Kresazan” for stain, which either google translates as “road traffic,” or it’s some sort of altered Azan stain)
What 2 parts of the pituitary gland do you see here?
Intermediate Part to the left
Neurohypophysis takes up the rest of the image
What tissue is this?
What important cell types/structures are here?
Neurohypophysis
Look for pituicytes: special glial cells, only their oval pale nucleus can be seen
I don’t think the Herring Bodies are supposed to be easily visible on the regular H-E stain, but with the chrome-hematoxylin-phloxin stain they should be dark greyish-blue, irregular spots
What tissue is this from?
What are the characteristic features you see of that tissue?
Pineal Gland
There are a few places of brain sand / acervulus
Also see pinealocytes with large, oval, granular nuclei and prominent nucleoli
Plus interstitial glial cells between pineaolocytes and blood vessels
What tissue is this?
What parts and cell types do you see?
Thyroid Gland
Look at answer image for identification
What tissue is this?
Thyroid Gland again
This time the image says “Autoradiographia”
What tissue is this?
What 2 cell types should you be able to identify?
Parathyroid Gland
Identify oxyphils and chief cells
What tissue is this?
What layer of that tissue makes up the majority of the image?
What is the thinner layer towards the bottom of the image?
And the lower-most layer?
Adrenal Cortex
(image is upside-down from the normal order)
Most of the image is Zona Fasciculata
Below that is Zona Glomerulosa
Then there’s the Capsule
What organ is this from?
What layer makes up the lower half of the image?
What layer is the top-right corner?
Adrenal Gland Cortex
Lower half of image is Zona Fasciculata
Top-right = Zona Reticularis
What tissue is this from?
What are the important layers/cells?
Adrenal Gland
Shows Zona Reticularis and Medulla
Notice the ganglion cells and chromaffin cells in the medulla
What tissue is this from?
What formation do you see?
Adrenal Gland Medulla
See the sinusoid capillary, feel the sinusoid capillary, know the sinusoid capillary
probably some other important stuff too
What tissue is this?
What stain?
What are the different layers of lining called?
Peripheral Nerve
Osmium Tetroxid stain
Outermost lining = epineurium, then there’s perineurium over each of the two balls, and endoneurium over individual nerve fibers
What is this tissue?
Peripheral Nerve
What’s this round blob here?
Peripheral Nerve
Can you spot the peripheral nerve in this one?
Go on, find it!
This is a scalp skin slide
What tissue is this?
Spinal Ganglion with Pseudounipolar Neurons
Very large round cell bodies with nuclei that are poorly stained except for their nucleoli
Perikarya should be basophilic due to Nissl substance
Note also the:
Satellite cells around the neurons
Schwann cells whose nuclei are among the nerve fibers
Wtf is this thing
Another example of pseudounipolar neurons but with silver staining
What tissue is this?
What stain?
Autonomic Ganglion with Multipolar Cells
Silver Nitrate Impregnation
The nuclei are “negatively stained”
What is the paler mass here?
This is a pancreas slide
This is labeled as a Vegetative / autonomic ganglion and does not really look like the alpha/beta cells of islets of Langerhans
What slide is this?
What stain?
Spinal Cord Cross-Section
I think this only shows half of the spinal cord, with the dorsal horn being in the lower half of the image (flipped from how you probably normally would draw it)
Cajal Silver stain
What are the most prominent type of cells you see here?
What slide might they be in (tissue and stain)
(Multipolar) Motor Neurons
Taken from the ventral horn of the spinal cord
Nissl stain (with cresyl-violet), which gives the rER a bluish-purple (Nissl Substance)
What tissue is this?
Cerebral Cortex
Remember the 6 layers
(could be worse, i mean it’s less layers than are in a burrito from taco bell)
Note that the image just has one pyramidal layer and also a ganglionic layer, but our department usually calls these the outer and inner pyramidal layers
What tissue is this image from?
What stain?
Cerebral Cortex
Bielschowsky Stain
Those 6 layers are more visible with this stain
Note that the image just has one pyramidal layer and also a ganglionic layer, but our department usually calls these the outer and inner pyramidal layers
What tissue is this from? What stain?
What notable cell is here?
Cerebral Cortex - Golgi stain
Pyramidal Cell
(maybe even a Betz cell, but hard to tell when it’s zoomed in)
What tissue is this from?
What is the light layer at the bottom and the darker layer above that?
What are the big dark cells at the border of those 2 layers?
Cerebellum
Light layer = Molecular Layer
Dark layer = Granule Cell Layer
Big cell in the border = Purkinje Cell, which make up a Purkinje Cell Layer aka Stratum Ganglionare
What tissue is this from? Stain?
What is the layer at the bottom and the darker layer above that?
What are the big dark cells at the border of those 2 layers?
Cerebellum - Bielschowsky stain
Light layer = Molecular Layer
Dark layer = Granule Cell Layer
Big cell in the border = Purkinje Cell, which make up a Purkinje Cell Layer aka Stratum Ganglionare
What type of cell is this?
What stain?
Purkinje Cell (in cerebellar cortex)
Golgi stain
There are 2 types of astrocytes here to find
It’s the fibrous and protoplasmic astrocytes
This is a Cajal gold impregnation stain
What is the green microsoft paint arrow pointing at?
a Meissner Corpuscle
This thing is fucking crazy, what is it?
A Pancreatic Paccinian Corpuscle
So your pancreas can feel vibration
What tissue is this?
What stain?
Motor End Plate
Acetylcholinesterase Histochemistry stain
You can lightly see the muscle fibers, while the dark splotches are the motor end plates
What tissue is this from?
There’s a ton of shit here, be able to find it
This is the Eye with the sclera, iris, etc
Make sure you know the Canal of Schlemm
What’s this tissue from?
What are all these layers?
Retina
What tissue is this from?
What stain?
Cochlea
Semithin Section, Toluidine Blue stain
What tissue is this from?
Can you identify some of the cells?
Organ of Corti
This image has all the important shit, but our school still tries to teach us all the names of the douchebags who wanted some phalangeal cells in the ear named after them like Hensen, Deiter, Claudius, and Boettcher
Corti was the only one of those dudes who mattered
What tissue is this from?
Lacrimal Gland
May be easy since this deck is separate from most of the other ones with gland cards, but it’s similar to parotid gland and pancreas
Although it’s exclusively serous, it has much less adipose than the parotid gland, and of course it doesn’t have islets of Langerhans
