Cytology Lab 1 Flashcards
The two most common LM stains are
- ) Hematoxalyn (blue)
2. ) Eosin (Red)
What had a thinner section, LM or EM?
EM
What is the theoretical limit of resolution for LM?
0.20 um
What is the theoretical limit of resolution for EM?
0.2-0.5 nm
A barrier between two aqueous compartments made up of choesterol, glycolipids, phospholipids, and proteins
Plasma membrane
Associated with nuclear lamins
Inner nuclear membrane
Continuous with endoplasmic reticulum. Can not distinguish a double membrane in LM
Outer nuclear membrane
We can not see mitochondria under LM using an
H and E stain
The golgi will appear with what type of stain?
Silver stain
With an H and E stain, the golgi is?
Unstained (will be a white region near nucleus)
Structures in the cytoplasm that are formed from the metbolic products of the cell
-non-living components of the cell
Inclusions (Glycogen, Lipids, Pigment granules)
The only inclusion with a membrane is the
Pigment granules
Membrane bound packets of stored products which have gone through the biosynthetic pathway
Secretory Granules
Cells storing many granules are indicative of
Regulated secretion
Light vacuolated appearance is indicative of
SER and Lipid Droplets
We can not see the cytoskeleton under
LM
Form the structural basis of microvilli, sterocilia, and adherent junctions
Actin
Involved in the intracellular trafficking of vesicles
Microtubules
Provide mechanical support for the cell
Intermediate filaments
Under LM, the RER stains
Blue
Lines all surfaces (external and internal) is avascular, and is highly cellular
Epithelium
Forms the parenchyma (secretory portion) of
exocrine and endocrine glands
Epithelium
Functions in protection and regulation of water and nutrient uptake
Apical plasma membrane
Which part of the epithelium provides protection to underlying connective tissue?
Skin
If the epithelium has one layer it is classified as
Simple
If the epithelium has two or more layers it is classified as
Stratified
Stratified in appearance, however, surface cells can be dome shaped or flat and the nuclei are ROUNDED in appearance
Transitional epithelium
All cells rest on the basement membrane, but not all cells reach the luminal surface
-Cell surface modifications (Cilia and Stereocilia) are ALWAYS present
Pseudostratified columnar epithelium
If present, should be included in the classification of
epithelial tissue.
Surface modifications and Goblet cells
Supports the tissue and forms the boundary between epithelium and connective tissue
Basement membrane or Basal Lamina