Culture media and Cultivation Flashcards
Types of Culture
Pure culture
mixed culture
Stock culture
One type of microbe
Pure culture
More than one type of microbe
Mixed culture
Unwanted microbe present
contaminated culture
does not contain agar. (BHI, TSB, and thioglycolate)
Liquid medium
contains 0.5%-1% agar (SIM medium)
Semi-solid medium
contains 2-3% agar
Solid medium
medium in which all the components are known
-isolation of cyanobacteria and chemoorganotrophs
Synthetic or defined medium
medium in which some substance are unknown
-very useful for the isolation of medically significant bacteria
non-synthetic or complex medium
used for obligate intracellular bacteria (rickettsia and chlamydia)
Tissue culture medium
distributed into the dish or plate
Plated media
prepared as either liquid, slant, butt and slant or butt.
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