1- GRAM POSITIVE SPORE-FORMING BACILLI CATALASE POSITIVE

Question 1

-causative agent of anthrax
- largest pathogenic bacteria
-are capable of forming

Answer 1

Bacillus

Question 2

MOT for bacillus

Answer 2

-inoculation
-injection
-ingestion
-inhalation

Question 3

inhibits phagocytosis; not polysaccharide capsule

Answer 3

Poly-glutamyl capsule

Question 4

impairs neutrophil function, and causes massive edema

Answer 4

Edema factor (EF)

Question 5

promotes entry of EF intro phagocytic cells

Answer 5

Protective antigen

Question 6

stimulates the production of TNF and IL-1

Answer 6

Lethal Factor

Question 7

four forms of disease

Answer 7

1. cutaneous anthrax
2. gastrointestinal anthrax
3. inhalational anthrax
4. injectional anthrax

Question 8

presumptive test for bacillus anthrax

Answer 8

immunochromatographic test

Question 9

Susceptibility test

Answer 9

string of pearl

Question 10

serotest to detect/ diagnose anthrax

Answer 10

Ascoli test

Question 11

-also known as Fried Rice bacillus
- found within the soil and widely distributed in
-opportunistic pathogen and often associated with food borne illness

Answer 11

Bacillus cereus

Question 12

two types of symptoms caused by bacillus cereus

Answer 12

Diarrheal, emetic

Question 13

box car morphology/ bamboo fishing rod

Answer 13

Bacillus anthracis

Question 14

-most common biological indicator
- hay bacillus
-ground glass appearance

Answer 14

Bacillus subtilis

Question 15

biological indicator of autoclave/ moist heat

Answer 15

Bacillus stearothermophilus

Question 16

causes Spastic paralysis

Answer 16

C. tetani

Question 17

causes Flaccid paralysis

Answer 17

C. botilinum

Question 18

clostridium welchii/ gas gangrene bacillus

Answer 18

C. perfringens

Question 19

virulence factor for Ctetani

Answer 19

tetanospasmin

-blocks the release of neurotransmitters causing spastic paralysis

Question 20

cardinal signs of tetanus

Answer 20

trismus / lock jaw
risus sardonicus/ devil’s grin
opisthotonus/ arching of the back

Question 21

gram staining $ microscopy of C. tetani

Answer 21

Gram neg with terminal spore tennis racket/ drum stick or lollipop appearance

Question 22

colonoy morphology of C. perfringens on 5%BAP

Answer 22

double zone or hemolysis/ target hemolysis

Question 23

positive for lecithinase

Answer 23

C. perfringens

Question 24

positive for lipase

Answer 24

C. botilinum

Question 25

the only non-motile organism of clostridium

Answer 25

C. perfringens

Question 26

horse manure odor

Answer 26

C. difficile