Controlling microbial growth in vitro Flashcards

1
Q

Factors that affect Microbial Growth

A
  1. Availability of nutrients
  2. Moisture
  3. Temperature
  4. pH
  5. Osmotic pressure
  6. Salinity
  7. barometric pressure
  8. gaseous atmosphere
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2
Q

removal of moisture or a dry environment

A

Dessication

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3
Q

is the temperature required by organisms to grow best

A

Optimum temperature

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4
Q

organisms that grow at high temperatures (they love heat)

examples: Cynobacteria

A

thermophiles

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5
Q

organisms that favor growth above 100c

examples: pyrolobus fumarii

A

Extreme thermophiles or Hyperthermophiles

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6
Q

organisms that grow at a moderate temperature (37c)

A

Mesophiles

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7
Q

cold loving organisms; grows best at-13c

examples: yersinia monocytogenes

A

psychrophiles

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8
Q

refrigerator temperature; optimum growth temperature of 4c

examples: bread molds

A

Psychotrops

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9
Q

prefer warm temperatures but can tolerate cold temperature

A

Psychoduric organisms

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10
Q

prefer a pH of 2-5

A

Acidophiles

examples: Lactobacillus acidophilus

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11
Q

prefer pH >8.5

A

Alkaliphiles

Example: Vibrio cholerae

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12
Q

pressure that is exerted on a cell membrane by solutions both inside and outside the cell

A

Osmotic pressure

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13
Q

Movement of solvent through a permeable membrane from a solution having a lower concentration to a higher concentration

A

osmosis

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14
Q

A solution with INCREASE concentration of solutes

A

hypertonic solution

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15
Q

bacterial cell membrane and cytoplasm SHRINK away from the bacterial cell wall

A

plasmolysis

happens when placed in a HYPERTONIC SOLUTION

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16
Q

A solution with DECREASE concentration of solutes

A

hypotonic solution

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17
Q

bacterial cell RUPTURES and the cytosol escapes

A

Plasmoptysis

happens when placed in a HYPOTONIC solution

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18
Q

Concentration of the fluid where the cells are suspended is equal to the concentration of the fluids inside the cells

A

isotonic solution

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19
Q

organisms that grown BEST at a salty environment ; “halo” salty

A

halophilic organisms

example: Vibrio spp

20
Q

organisms that DO NOT prefer salty environment but CAN SURVIVE

A

haloduric organisms

example; Staphyloccocus Aureus

21
Q

microorganisms that survive deep in the ocean or in oil wells where atmospheric pressure is very high

A

Piezophiles

22
Q

Organisms which REQUIRE OXYGEN to live

A

Obligate aerobes

23
Q

bacteria that grow on the ABSENCE of atmospheric or free oxygen and obtain oxygen from oxygen-containing compound

A

Obligate anaerobes

Examples : Mycobacterium and micrococus

24
Q

an anaerobes, but can grow in the PRESENCE of atmospheric oxygen

A

Facultative aerobes

25
Q

Require increase Carbon dioxide

A

Capnophiles

26
Q

DOES NOT GROW WELL, but survives in the presence of atmospheric oxygen q

A

Aerotolerant

27
Q

Requires REDUCED oxygen

A

Microaerophiles

28
Q

Artificial or synthetic media used in the laboratory to grow microorganisma

A

Culture media

29
Q

destruction of or elimination of all microbes, including cells, spores, and viruses

A

Sterilization

30
Q

elimination of most or all pathogens except bacterial spores, form nonliving objects

A

Disinfection

31
Q

Chemical used to disinfect inanimate objects are called

A

Disinfectant

32
Q

Chemicals used to disinfect skin and other living tissues are calles

A

Antiseptic

33
Q

method used to disinfect liquids, milk, wine, and other beverages

A

pasteurization

34
Q

Reduction of bacterial population to levels considered safe by Public Health Standard

A

Sanitization

35
Q

Drugs or chemical that KILL bacteria

A

bactericidal Agents

36
Q

Used to kill bacterial Endospore

A

Sporicidal agents

37
Q

drugs or chemical that can inhibit growth and multiplication of bacteria

A

bacteriostatic agents

38
Q

The process of dehydration (drying) followed by freezing to preserve the bacteria for future use and study

A

lyophilization

39
Q

Refers to the presence of pathogen in blood or tissue

A

Sepsis

40
Q

absence of pathogen

A

Asepsis

41
Q

is the prevention of infection

A

Antisepsis

42
Q

Mechanical removal of microbes rather than killing from a limited area, for example the skin around an injection site

A

Degerming/Degermation

43
Q

the time necessary to kill microorganisms is DIRECTLY PROPORTIONAL to the number of microorganisms present

A

TRUE

44
Q

hard water may REDUCE the rate of killing microorganisms

A

TRUE

45
Q

70% alcohol is more effective that 95% alcohol due to its water content

A

TRUE