Controlling microbial growth in vitro Flashcards
Factors that affect Microbial Growth
- Availability of nutrients
- Moisture
- Temperature
- pH
- Osmotic pressure
- Salinity
- barometric pressure
- gaseous atmosphere
removal of moisture or a dry environment
Dessication
is the temperature required by organisms to grow best
Optimum temperature
organisms that grow at high temperatures (they love heat)
examples: Cynobacteria
thermophiles
organisms that favor growth above 100c
examples: pyrolobus fumarii
Extreme thermophiles or Hyperthermophiles
organisms that grow at a moderate temperature (37c)
Mesophiles
cold loving organisms; grows best at-13c
examples: yersinia monocytogenes
psychrophiles
refrigerator temperature; optimum growth temperature of 4c
examples: bread molds
Psychotrops
prefer warm temperatures but can tolerate cold temperature
Psychoduric organisms
prefer a pH of 2-5
Acidophiles
examples: Lactobacillus acidophilus
prefer pH >8.5
Alkaliphiles
Example: Vibrio cholerae
pressure that is exerted on a cell membrane by solutions both inside and outside the cell
Osmotic pressure
Movement of solvent through a permeable membrane from a solution having a lower concentration to a higher concentration
osmosis
A solution with INCREASE concentration of solutes
hypertonic solution
bacterial cell membrane and cytoplasm SHRINK away from the bacterial cell wall
plasmolysis
happens when placed in a HYPERTONIC SOLUTION
A solution with DECREASE concentration of solutes
hypotonic solution
bacterial cell RUPTURES and the cytosol escapes
Plasmoptysis
happens when placed in a HYPOTONIC solution
Concentration of the fluid where the cells are suspended is equal to the concentration of the fluids inside the cells
isotonic solution