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SBB > Component Prepartion > Flashcards

Component Prepartion Flashcards

1
Q

How long is WB good for in ACD or CPD anticoagulant?

A

21 days

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2
Q

How long is WB good for in CDPA-1?

A

35 days

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3
Q

How much plasma is removed from whole blood collected in CPDA-1 when making RBC components?

A

If CPDA-1 is used, 200 to 250 mL of plasma can be removed, leaving 150-230 ml of RBC product with a hematocrit of 65% to 80%

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4
Q

How much plasma is removed from whole blood when making RBC components with additive solutions?

A

If additive solutions (AS) are employed, an additional 50 mL of plasma can be removed, because 150 mL of adenine-saline is added back to the cells, achieving the desired hematocrit level of less than 80%. Typically, RBCs with AS added will have a hematocrit of 55% to 65%.

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5
Q

How long is WB centrifuged when making RBC components?

A

Centrifuge whole blood using a heavy spin (5,000 × g for 5 minutes in a refrigerated centrifuge). Temperature setting should be 4°C. If also preparing platelet conc., the initial spin will be light (2 to 3 minute at 3,200 rpm) at 20-24C

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6
Q

How is the QC on HCT of RBC units done?

A

Quality control on the hematocrit level of the RBCs is performed monthly;
75% of samples tested must yield a hematocrit level of 80% or less.

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7
Q

What is the min and max dose for irradiation?

A

Both the FDA and AABB recommend a minimum dose of gamma irradiation of 25 Gy to the central portion of the blood unit, with no less than 15 Gy delivered to any part of the blood unit

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8
Q

What is the outdate of the product after irradiation?

A

28 days or the expiration of the unit whichever is sooner

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9
Q

What is the AABB standard for leukoreduced RBC’s?

A

The absolute WBC count in the unit is reduced to less than 5 × 10^6 and contains at least 85% of the original RBC mass in 95% of units tested.

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10
Q

What are prestorage leukocyte filters made of?

A

multiple layers of polyester or cellulose acetate nonwoven fibers that trap leukocytes and platelets but that allow RBCs to flow through

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11
Q

What is the big advantage of pre-storage leukocyte filters?

A

Reduces biological response modifers such as proinflammatory cytokines (interleukin-1, interleukin-6, and tumor necrosis factor) and complement fragments (C5a and C3a).

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12
Q

When must RBC’s be frozen?

A

RBCs are frozen within 6 days of collection when the preservative is CPD or CPDA-1 and up to 42 days when preserved in AS-1, AS-3, and AS-5 and rare unit
CPD, CDA-1 RBCs: rejuvenate up to 3 days after expiration. Wash & transfused within 24 hours or freeze up to10 years

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13
Q

Once Glycerol is added how long until the unit must be placed in the freezer?

A

4 hours

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14
Q

What is the advantage of using high glycerol?

A

40% glycerol allows for slow uncontrolled freezing and less sensitive and no fancy equipment required. Units can be stored in -80C mechanical freezer

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15
Q

What are the disadvantages of using low glycerol?

A

20% glycerol is less protective and a very rapid, more controlled freezing procedure is required. Liquid nitrogen (N ) is routinely used for this method. The frozen units must be stored at about -120°

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16
Q

How long after expiration can units be frozen?

A

RBCs can be rejuvenated up to 3 days after expiration and then glycerolized and frozen.

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17
Q

What is the minimum number of platelets in apheresis donor?

A

3 x 10^11 in 90% of units tested

with < 5x10^6 WBC in 95% units tested

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18
Q

What is the acceptable pH of plt’s?

A

greater than or equal to 6.2 in 90% of units tested

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19
Q

What is the min number of platelets in random donor concentrates?

A

5.5 x10^10 in 90% units tested or 75% if leukoreduced

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20
Q

How are plt concentrates prepared from whole blood?

A

Light spin separates PRP from RBC’s. PRP is expressed and hard spin concentrates PLT’s at the bottom. Plasma is removed and platelets rest for 1-2 hours until clumps resuspend.

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21
Q

How much fibrinogen is one unit of plasma?

A

approx. 400 mg of fibrinogen

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22
Q

How much clotting factors are in one unit of FFP?

A

1 IU per 1 mL of plasma

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23
Q

After thaw how long is FFP good for?

A

24 hours unless labeled as thawed plasma then 5 days

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24
Q

What factors are missing from cryo poor plasma?

A

VIII, Fibrinogen, XIII, fibronectin, Cryoglobulin, vWF

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25
Q

What factors are slightly reduced in thawed plasma?

A

V, VII, VIII, and X

26
Q

How long is liquid plasma good for?

A

5 days after the expiration date of whole blood.

27
Q

How much factor VIII and fibrinogen are in one unit of cryo

A

At least 80 units of VIII and 150 mg of fibrinogen

Also has XIII, vWF, and fibronectin

28
Q

How long is cryo good for after thawing?

A

Cryo expires 6 hours after thawing or 4 hours after pooling

29
Q

What are the QC requirements for cryo?

A

Volume should exceed 25 ml and 75% of all units must have 80 IU of VIII

30
Q

How many whole blood derived platelets, FFP, and cryo must be tested?

A

At least 4 units should be tested monthly

31
Q

What is the AABB standard for number of platelets in a apheresis unit?

A

> 3 x 1011 platelets in 90% of units tested

32
Q

What is the AABB standard for the pH on apheresis plt’s?

A

pH > 6.2 at end of allowable storage in 90% of units tested

33
Q

What is the AABB standard for the number of WBC’s in an apheresis PLT?

A

95% of units sampled contain a residual leukocyte count <5 x 106.

34
Q

What drugs affect platelet function and cause a deferral?

A

Aspirin in past 2 days (36 hours min)
ASA containing drugs such as Feldene = 2 days
Plavix (Clopidogrel) or Ticlid (Ticlopidine): no medication for 14 days prior to donation

35
Q

How often can you donate apheresis plt’s?

A

Minimum of 2 days between Plt donations
No more than 2 Plt donations/week
No more than 24 Plt donations/year

36
Q

How often does a plt count need to be performed before plt apheresis procedure?

A

Although plt count is not needed prior to the 1st plateletpheresis procedure, a plt count does need to be performed on a sample collected immediately before the procedure or from a sample collected either before or after the previous procedure.
If more than 4 weeks have elapsed between platelet donations, the platelet count does NOT have to be determined prior to donation.

37
Q

How much plasma can be removed during an plt apheresis?

A

< 500mL of plasma can be removed with each procedure

Can be < 600mL if donor weighs > 175lbs

38
Q

How much RBC’s can be removed with out deferral?

A

Less than 100 mL removed = no deferral
If RBC can’t be returned the donor is deferred for 8 weeks.
8 week deferral can be waived if MD approves and plt’s are special

39
Q

What is an infrequent or occasional plasma donor?

A

Donates less than every 4 weeks

40
Q

What is a frequent or serial plasma donor?

A

Donates greater than every 4 weeks

41
Q

In a serial plasma donor what is the maximum number of RBC’s lost per week?

A

must be less than 25 mL of RBC’s/ week

42
Q

How often can a plasmapheresis be performed?

A

At least 2 days between procedures
No more than 2 procedures in a 7 day period
No more than 12 L/year (14.4 L if weight >175lbs)

43
Q

How often do Total protein + serum protein electrophoresis or quantitative immunoglobulins (IgG, IgM, IgA) need to be tested?

A

Prior to first procedure AND once every 4 months
Annual physical exam
Total protein must be > 6.0 g/dL

44
Q

What is jumbo FFP?

A

Typically equivalent of 3 WB-derived FFP
Volume > 450mL
Storage requirements & expiration are same as for WB-derived FFP

45
Q

What is the donor criteria for a double RBC donation?

A 
Hct > 40% (NOT Hgb)
Males:  at least 5'1″ and > 130 lbs
Females:  at least 5'5″ and >150 lbs
Deferred 16 weeks
Saline must be used to replace volume
46
Q

What is the QC standard for apheresis RBC collection?

A

Initially 100 consecutive RBCs must be tested according to manufacturer’s parameters
If 95% meet criteria, test 50 units/month for absolute RBC volume with 95% acceptance rate

47
Q

What is the minimum number of granulocytes that should be collected?

A

Should be > 1 x 10^10 granulocytes in 75% of units tested

48
Q

How do you calculate maximum Extracorporeal Volume (ECV)?

A

15% of total blood volume
150 lb male patient = 68.2 kg x 66 ml/kg = 4500 mL TBV
4500 mL x 0.15 = 675 mL (max ECV)

49
Q

When should RBC’s be frozen?

A

AABB recomends within 6 days of collection

50
Q

When can RBC’s be rejuvenated?

A

Within 3 days of expiration of component when using CPD and CPDA-1
or up to 42 day expiration with CPD/(AS)-1

51
Q

Which anticoagulants is rejuvesol not approved for?

A

CPD/AS-3 and CPD/AS-5

52
Q

What is the expiration of the unit if Haemonetics ACP 215 Automated Cell Processor was used for deglyceroling

A

closed system = 14 days

53
Q

How do you determine if glycerol as removed>?

A

Measure the osmolality of the deglycerolized rbc supernatant
420-500 mOsm
Refractometer
specific gravity of <1.3384 correlate with glycerol levels below 1%

54
Q

Calculate RBC recovery after deglyceroling

A

% Red Cell Recovery =
weight (g) x HCT of DRBC
weight (g) x HCT of original RBC

Recovery of 80% or greater is acceptable/

55
Q

How long after whole blood collection do you have to add additive solutions?

A

72 hours

56
Q

What does storage do to 2,3 DPG?

A

2,3 DPG decreases in storage
Less O2 is released to tissues
Increased O2 affinity

57
Q

What do rejuvenating solutions contain?

A

Pyruvate, Inosine, Phosphate, Adenine

58
Q

What do rejuvenating solutions do?

A

Restore ATP and 2,3 DPG

59
Q

What happens to RBC’s if frozen without cryopreservation?

A

Slow freezing results in extracellular water freezing before intracellular causing dehydration and tonic lysis of cells
Rapid freezing creates ice crystals that damage cells

60
Q

How are Gycerlized RBC’s thawed?

A

Thawed in 37 water bath then washed with 12% saline and ending with 0.9% saline + dextrose

SBB (37 decks)

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