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MLT Program > Clinical Microbiology Exam 3 > Flashcards

Clinical Microbiology Exam 3 Flashcards

1
Q

5 characteristics of all enterobacterales

A

oxidase -
GNB or coccobacilli
glucose fermenting
nitrate reducing
faculcative anaerobes

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2
Q

what is TSI and what sugars are included

A

sugar fermenter, gas, h2s
glucose, sucrose, lactose

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3
Q

urea pos

A

proteus, kleb, provid, morganella

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4
Q

Plesimonas shigelloides

A

GS: GNB
Strings test: neg
vibriostatic agent 0/129: susceptible

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5
Q

enterobacterales is normal flora where?

A

GI tract

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6
Q

motility neg

A

kleb, shigella

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7
Q

morph on Xylose-Lysine Deoxycholate

A

salmonella, shigella: black H2S production

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8
Q

Serratia

A

MAC: red
ONPG: pos
DNase: pos (distinctly pos for Serratia)

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9
Q

phenylalanine deaminase pos

A

proteus, provid, morganella

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10
Q

Escherichia coli identification

A

MAC: flat, dark pink
IMViC: ++–
Virulence Factor: pili for GI attachment

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11
Q

what reagents are added to nitrate reduction

A

reagent A (sulfanilic acid)
reagent B (alpha naphthylamine)
zinc dust

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12
Q

dnase pos

A

seratia

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13
Q

Enterotoxigenic E coli

A

traveller’s diarrhea

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14
Q

where are oxidase pos glucose fermenters found

A

water, coasts, salt water

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15
Q

Capsular antigen

A

K

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16
Q

yersinia

A

infections: plague
GS: safety pin, gram neg
unique incubation: room temp

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17
Q

primary pathogens from enterobacterales

A

shigella spp, salmonella enteritica, yersinia spp

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18
Q

what reagents are added methyl red

A

glucose fermentation, add methyl red

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19
Q

Psuedomonas aeruginosa

A

oxidase: pos
utilize carbohydrates: obligate aerobe/oxidation
odor: grapes/tortilla chips
BAP: feathered, metallic sheen
pigment: green, red, blue, yellow (pio-)
Growth temp: 42C (only pseudomonas to grow)
infections: swimmer’s ear, burns, jacuzzi syndrome

20
Q

oxidase neg, non-fermenters

A

stenotrophamonous, asinitobacter

21
Q

vp pos

A

kleb, entero, seratia

22
Q

TSI positives

A

A/A (yellow/yellow) - glucose + sucrose or lactose
K/A (pink/yellow) - glucose ferm
K/K (pink/pink) - no ferm

23
Q

oxidase pos, glucose fermenters

A

plesiomonus, aeromonus, vibrio

24
Q

E coli O157:H7

A

SMAC plate, sorbitol pos

25
Q

Enterobacter

A

MAC: wet pink (mucoid)
IMViC: –++
ONPG: pos

26
Q

Enteropathogenic E coli

A

day cares, kids 1 and younger

27
Q

flagellar antigen

A

H

28
Q

What organisms fall under class B bioterrorism?

A

Burkholderia mallei, Burkholderia psuedomallei

29
Q

h2s pos

A

proteus, salmonella

30
Q

acinetobacter

A

GS: small GN coccobacilli

31
Q

ornithine neg

A

kleb, provid

32
Q

what grows on MacConkey Sorbitol

A

O157:H7

33
Q

morph. on Hektoen Enteric agar

A

salmonella: black
shigella: clear/blue-green

34
Q

Aeromonas

A

GS: GNB
Indole: pos
String test: neg
Vibriostatic agent 0/129: resistant

35
Q

Enterohemorrhagic E coli

A

undercooked meat

36
Q

Shigella

A

sonnea most common in US
glucose fermenter
neg for all else

37
Q

somatic antigen

A

o (cell wall)

38
Q

Vibrio

A

GS: small curved GNB
String: pos (distinctly pos for Vibrio)
vibriostatic agent 0/129: susceptible
symptoms: rice water
virulence factor: cholera toxin (dehydration)
media: TCBSq

39
Q

what grows on CIN agar

A

Yersinia @ room temp

40
Q

Burkholderia cepacia infects who?

A

cystic fibrosis patients

41
Q

Salmonella

A

stool pathogen
H2S producer

42
Q

stool pathogens

A

e. coli O157:H7, salmonella, vibrio, shigella, yersinia

43
Q

Proteus

A

MAC: clear
BAP: swarming
Indole: pos (vulgaris), neg (mirabilis)

44
Q

what is ONPG

A

detect slow lactose fermenters

45
Q

morph. on MacConkey

A

salmonella, shigella: both clear

46
Q

what reagents are added to VP

A

A (alpha naphthol)
B (KOH)

47
Q

Klebsiella identification

A

MAC: mucoid pink
IMViC: –++ (pnuemo), +-++ (oxy)
Virulence Factor: capsules
Indole: neg (pnuemo), pos (oxy)

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