Clinical Microbiology Exam 3 Flashcards
5 characteristics of all enterobacterales
oxidase -
GNB or coccobacilli
glucose fermenting
nitrate reducing
faculcative anaerobes
what is TSI and what sugars are included
sugar fermenter, gas, h2s
glucose, sucrose, lactose
urea pos
proteus, kleb, provid, morganella
Plesimonas shigelloides
GS: GNB
Strings test: neg
vibriostatic agent 0/129: susceptible
enterobacterales is normal flora where?
GI tract
motility neg
kleb, shigella
morph on Xylose-Lysine Deoxycholate
salmonella, shigella: black H2S production
Serratia
MAC: red
ONPG: pos
DNase: pos (distinctly pos for Serratia)
phenylalanine deaminase pos
proteus, provid, morganella
Escherichia coli identification
MAC: flat, dark pink
IMViC: ++–
Virulence Factor: pili for GI attachment
what reagents are added to nitrate reduction
reagent A (sulfanilic acid)
reagent B (alpha naphthylamine)
zinc dust
dnase pos
seratia
Enterotoxigenic E coli
traveller’s diarrhea
where are oxidase pos glucose fermenters found
water, coasts, salt water
Capsular antigen
K
yersinia
infections: plague
GS: safety pin, gram neg
unique incubation: room temp
primary pathogens from enterobacterales
shigella spp, salmonella enteritica, yersinia spp
what reagents are added methyl red
glucose fermentation, add methyl red
Psuedomonas aeruginosa
oxidase: pos
utilize carbohydrates: obligate aerobe/oxidation
odor: grapes/tortilla chips
BAP: feathered, metallic sheen
pigment: green, red, blue, yellow (pio-)
Growth temp: 42C (only pseudomonas to grow)
infections: swimmer’s ear, burns, jacuzzi syndrome
oxidase neg, non-fermenters
stenotrophamonous, asinitobacter
vp pos
kleb, entero, seratia
TSI positives
A/A (yellow/yellow) - glucose + sucrose or lactose
K/A (pink/yellow) - glucose ferm
K/K (pink/pink) - no ferm
oxidase pos, glucose fermenters
plesiomonus, aeromonus, vibrio
E coli O157:H7
SMAC plate, sorbitol pos
Enterobacter
MAC: wet pink (mucoid)
IMViC: –++
ONPG: pos
Enteropathogenic E coli
day cares, kids 1 and younger
flagellar antigen
H
What organisms fall under class B bioterrorism?
Burkholderia mallei, Burkholderia psuedomallei
h2s pos
proteus, salmonella
acinetobacter
GS: small GN coccobacilli
ornithine neg
kleb, provid
what grows on MacConkey Sorbitol
O157:H7
morph. on Hektoen Enteric agar
salmonella: black
shigella: clear/blue-green
Aeromonas
GS: GNB
Indole: pos
String test: neg
Vibriostatic agent 0/129: resistant
Enterohemorrhagic E coli
undercooked meat
Shigella
sonnea most common in US
glucose fermenter
neg for all else
somatic antigen
o (cell wall)
Vibrio
GS: small curved GNB
String: pos (distinctly pos for Vibrio)
vibriostatic agent 0/129: susceptible
symptoms: rice water
virulence factor: cholera toxin (dehydration)
media: TCBSq
what grows on CIN agar
Yersinia @ room temp
Burkholderia cepacia infects who?
cystic fibrosis patients
Salmonella
stool pathogen
H2S producer
stool pathogens
e. coli O157:H7, salmonella, vibrio, shigella, yersinia
Proteus
MAC: clear
BAP: swarming
Indole: pos (vulgaris), neg (mirabilis)
what is ONPG
detect slow lactose fermenters
morph. on MacConkey
salmonella, shigella: both clear
what reagents are added to VP
A (alpha naphthol)
B (KOH)
Klebsiella identification
MAC: mucoid pink
IMViC: –++ (pnuemo), +-++ (oxy)
Virulence Factor: capsules
Indole: neg (pnuemo), pos (oxy)
