Clinical Microbiology Exam 3

Question 1

5 characteristics of all enterobacterales

Answer 1

oxidase -
GNB or coccobacilli
glucose fermenting
nitrate reducing
faculcative anaerobes

Question 2

what is TSI and what sugars are included

Answer 2

sugar fermenter, gas, h2s
glucose, sucrose, lactose

Question 3

urea pos

Answer 3

proteus, kleb, provid, morganella

Question 4

Plesimonas shigelloides

Answer 4

GS: GNB
Strings test: neg
vibriostatic agent 0/129: susceptible

Question 5

enterobacterales is normal flora where?

Answer 5

GI tract

Question 6

motility neg

Answer 6

kleb, shigella

Question 7

morph on Xylose-Lysine Deoxycholate

Answer 7

salmonella, shigella: black H2S production

Question 8

Serratia

Answer 8

MAC: red
ONPG: pos
DNase: pos (distinctly pos for Serratia)

Question 9

phenylalanine deaminase pos

Answer 9

proteus, provid, morganella

Question 10

Escherichia coli identification

Answer 10

MAC: flat, dark pink
IMViC: ++–
Virulence Factor: pili for GI attachment

Question 11

what reagents are added to nitrate reduction

Answer 11

reagent A (sulfanilic acid)
reagent B (alpha naphthylamine)
zinc dust

Question 12

dnase pos

Answer 12

seratia

Question 13

Enterotoxigenic E coli

Answer 13

traveller’s diarrhea

Question 14

where are oxidase pos glucose fermenters found

Answer 14

water, coasts, salt water

Question 15

Capsular antigen

Answer 15

K

Question 16

yersinia

Answer 16

infections: plague
GS: safety pin, gram neg
unique incubation: room temp

Question 17

primary pathogens from enterobacterales

Answer 17

shigella spp, salmonella enteritica, yersinia spp

Question 18

what reagents are added methyl red

Answer 18

glucose fermentation, add methyl red

Question 19

Psuedomonas aeruginosa

Answer 19

oxidase: pos
utilize carbohydrates: obligate aerobe/oxidation
odor: grapes/tortilla chips
BAP: feathered, metallic sheen
pigment: green, red, blue, yellow (pio-)
Growth temp: 42C (only pseudomonas to grow)
infections: swimmer’s ear, burns, jacuzzi syndrome

Question 20

oxidase neg, non-fermenters

Answer 20

stenotrophamonous, asinitobacter

Question 21

vp pos

Answer 21

kleb, entero, seratia

Question 22

TSI positives

Answer 22

A/A (yellow/yellow) - glucose + sucrose or lactose
K/A (pink/yellow) - glucose ferm
K/K (pink/pink) - no ferm

Question 23

oxidase pos, glucose fermenters

Answer 23

plesiomonus, aeromonus, vibrio

Question 24

E coli O157:H7

Answer 24

SMAC plate, sorbitol pos

Question 25

Enterobacter

Answer 25

MAC: wet pink (mucoid) IMViC: --++ ONPG: pos

Question 26

Enteropathogenic E coli

Answer 26

day cares, kids 1 and younger

Question 27

flagellar antigen

Answer 27

H

Question 28

What organisms fall under class B bioterrorism?

Answer 28

Burkholderia mallei, Burkholderia psuedomallei

Question 29

h2s pos

Answer 29

proteus, salmonella

Question 30

acinetobacter

Answer 30

GS: small GN coccobacilli

Question 31

ornithine neg

Answer 31

kleb, provid

Question 32

what grows on MacConkey Sorbitol

Answer 32

O157:H7

Question 33

morph. on Hektoen Enteric agar

Answer 33

salmonella: black shigella: clear/blue-green

Question 34

Aeromonas

Answer 34

GS: GNB Indole: pos String test: neg Vibriostatic agent 0/129: resistant

Question 35

Enterohemorrhagic E coli

Answer 35

undercooked meat

Question 36

Shigella

Answer 36

sonnea most common in US glucose fermenter neg for all else

Question 37

somatic antigen

Answer 37

o (cell wall)

Question 38

Vibrio

Answer 38

GS: small curved GNB String: pos (distinctly pos for Vibrio) vibriostatic agent 0/129: susceptible symptoms: rice water virulence factor: cholera toxin (dehydration) media: TCBSq

Question 39

what grows on CIN agar

Answer 39

Yersinia @ room temp

Question 40

Burkholderia cepacia infects who?

Answer 40

cystic fibrosis patients

Question 41

Salmonella

Answer 41

stool pathogen H2S producer

Question 42

stool pathogens

Answer 42

e. coli O157:H7, salmonella, vibrio, shigella, yersinia

Question 43

Proteus

Answer 43

MAC: clear BAP: swarming Indole: pos (vulgaris), neg (mirabilis)

Question 44

what is ONPG

Answer 44

detect slow lactose fermenters

Question 45

morph. on MacConkey

Answer 45

salmonella, shigella: both clear

Question 46

what reagents are added to VP

Answer 46

A (alpha naphthol) B (KOH)

Question 47

Klebsiella identification

Answer 47

MAC: mucoid pink IMViC: --++ (pnuemo), +-++ (oxy) Virulence Factor: capsules Indole: neg (pnuemo), pos (oxy)