Clin Microbiology Final

Question 1

Blood Agar Plates are an example of which of the following?

Answer 1

Differential and nonselective

Question 2

The most common method of physically treating infectious waste

Answer 2

Incineration

Question 3

Which of the following types of media can detect hemolysis?

Answer 3

Columbia-Colistin Nalidixic Acid Agar (CNA),
5% Sheep Blood Agar (SBA)

Question 4

Bacteria that can grow better anaerobically but will grow in the presence of oxygen are called

Answer 4

Facultative anaerobes

Question 5

Colony shape can be described using which morphologic term?

Answer 5

Round

Question 6

Which of the following is NOT an application of Microscopy in Diagnostic Microbiology?

Answer 6

Determining whether the antimicrobial susceptibility profiles are unusual or aberrant for a particular bacterial species

Question 7

Environmental conditions for growth of bacteria include all of the following

Answer 7

Temperature, pH, Nutritional requirements, Oxygen and carbon dioxide availability

Question 8

Which of the following do not use fermentation as a form of energy production?

Answer 8

Obligate aerobes

Question 9

Which of the following is acceptable nomenclature for staphylococcus aureus?

Answer 9

Staphylococcus aureus,
S. aureus

Question 10

Media that is used to distinguish the types of bacteria that are able to ferment certain sugars or distinguish the type of hemolysis they exhibit:

Answer 10

Differential agar

Question 11

Lactobacilli in the urogenital tract is an example of

Answer 11

Mutualism

Question 12

Which of the following has a waxy substance (mycolic acids) in its cell wall?

Answer 12

Acid fast organisms

Question 13

List the proper labeling criteria that are needed for microbial specimens to be accepted in the microbiology lab setting.

Answer 13

Patient name and date of birth, time/date of collection, specimen source

Question 14

Which of the following can NOT be tossed into regular trash?

Answer 14

Glass slides

Question 15

In a gram stain, what color(s) will a gram-positive organism stain, and what color(s) will a gram-negative organism stain?

Answer 15

Gram positive are violet, Gram negative are pink.

Question 16

What is the optimal temperature for growing most human bacterial pathogens?

Answer 16

35-37C (Body Temp)

Question 17

Which media should be inoculated first?

Answer 17

5% Sheep Blood Agar (SBA)

Question 18

Name one physical barrier that is the bodies “first line of defense”?

Answer 18

Mucous membranes.

Question 19

Which of the following microbes are considered Prokaryotic?

Answer 19

Bacteria

Question 20

MacConkey Agar can detect the fermentation of what?

Answer 20

Glucose

Question 21

For the organism Staphylococcus aureus, what is the genus name?

Answer 21

Staphylococcus

Question 22

Sterilization is the process that:

Answer 22

Kills all forms of microbial life, including spores

Question 23

MALDI-TOF assays that are performed in the clinical laboratory setting are done so through which type of testing methodology?

Answer 23

Fluorometry

Question 24

Flagella are exterior protein filaments that rotate and cause bacteria to be motile. Which of the following are types of flagella?

Answer 24

BiPolar, MonoPolar, Peritrichous

Question 25

Which of the following is NOT an example of a Phenotypic Characteristic used to identify bacteria?

Answer 25

Nucleic acid sequence

Question 26

Streaking a urine culture uses

Answer 26

A calibrated loop

Question 27

Which of the following statements concerning bacterial cell walls is incorrect?

Answer 27

Gram-negative bacterial cell walls contain no peptidoglycan

Question 28

Which media type would inhibit gram-negative organisms, but encourage growth of gram-positive?

Answer 28

Selective agar

Question 29

Bacteria have different morphologic shapes. Which pairing of shapes is incorrect?

Answer 29

Diphtheroid are pairs

Question 30

When using the isolation streaking technique, the most isolated forms of the organism should be in which quadrant?

Answer 30

Fourth

Question 31

All of the following can be detected using bright-field microscopy, except for?

Answer 31

Viruses