Clin Microbiology Final Flashcards
Blood Agar Plates are an example of which of the following?
Differential and nonselective
The most common method of physically treating infectious waste
Incineration
Which of the following types of media can detect hemolysis?
Columbia-Colistin Nalidixic Acid Agar (CNA),
5% Sheep Blood Agar (SBA)
Bacteria that can grow better anaerobically but will grow in the presence of oxygen are called
Facultative anaerobes
Colony shape can be described using which morphologic term?
Round
Which of the following is NOT an application of Microscopy in Diagnostic Microbiology?
Determining whether the antimicrobial susceptibility profiles are unusual or aberrant for a particular bacterial species
Environmental conditions for growth of bacteria include all of the following
Temperature, pH, Nutritional requirements, Oxygen and carbon dioxide availability
Which of the following do not use fermentation as a form of energy production?
Obligate aerobes
Which of the following is acceptable nomenclature for staphylococcus aureus?
Staphylococcus aureus,
S. aureus
Media that is used to distinguish the types of bacteria that are able to ferment certain sugars or distinguish the type of hemolysis they exhibit:
Differential agar
Lactobacilli in the urogenital tract is an example of
Mutualism
Which of the following has a waxy substance (mycolic acids) in its cell wall?
Acid fast organisms
List the proper labeling criteria that are needed for microbial specimens to be accepted in the microbiology lab setting.
Patient name and date of birth, time/date of collection, specimen source
Which of the following can NOT be tossed into regular trash?
Glass slides
In a gram stain, what color(s) will a gram-positive organism stain, and what color(s) will a gram-negative organism stain?
Gram positive are violet, Gram negative are pink.
What is the optimal temperature for growing most human bacterial pathogens?
35-37C (Body Temp)
Which media should be inoculated first?
5% Sheep Blood Agar (SBA)
Name one physical barrier that is the bodies “first line of defense”?
Mucous membranes.
Which of the following microbes are considered Prokaryotic?
Bacteria
MacConkey Agar can detect the fermentation of what?
Glucose
For the organism Staphylococcus aureus, what is the genus name?
Staphylococcus
Sterilization is the process that:
Kills all forms of microbial life, including spores
MALDI-TOF assays that are performed in the clinical laboratory setting are done so through which type of testing methodology?
Fluorometry
Flagella are exterior protein filaments that rotate and cause bacteria to be motile. Which of the following are types of flagella?
BiPolar, MonoPolar, Peritrichous
Which of the following is NOT an example of a Phenotypic Characteristic used to identify bacteria?
Nucleic acid sequence
Streaking a urine culture uses
A calibrated loop
Which of the following statements concerning bacterial cell walls is incorrect?
Gram-negative bacterial cell walls contain no peptidoglycan
Which media type would inhibit gram-negative organisms, but encourage growth of gram-positive?
Selective agar
Bacteria have different morphologic shapes. Which pairing of shapes is incorrect?
Diphtheroid are pairs
When using the isolation streaking technique, the most isolated forms of the organism should be in which quadrant?
Fourth
All of the following can be detected using bright-field microscopy, except for?
Viruses
