CLINICAL HISTOCOMPATIBILITY TESTING

Question 1

• Specialized laboratory procedures used to determine the compatibility between a transplant donor and recipient.

• The goal of clinical histocompatibility testing is to minimize the risk of rejection and improve the chances of successful transplantation.

Answer 1

CLINICAL HISTOCOMPATIBILITY
TESTING

Question 2

• Types:

Answer 2

• HLA typing
• HLA phenotyping
• HLA genotyping

• HLA antibody screening and identification.

Question 3

• Identification of HLA antigens or genes in transplant candidates or donors.
• Determines phenotypes or genotypes of classical transplant antigens/genes.

Answer 3

HLA TYPING

Question 4

• Purpose:
• To identify the most suitable donor-recipient pair from an immunologic perspective.

• Other Donor Selection Considerations:
ABO compatibility
Infectious disease status

Answer 4

HLA TYPING

Question 5

Key HLA Antigens/Genes Tested:

Answer 5

HLA-A, HLA-B, HLA-Cw, HLA-DR, HLA-DQ

Question 6

HLA PHENOTYPING

• Uses antisera/antibodies to detect HLA antigens on lymphocytes.
• T and B cells for HLA class I; purified B cells for HLA class II.

Answer 6

• Complement-dependent cytotoxicity (CDC) test

Question 7

• Key Steps:
• Incubate lymphocytes with specific antisera in a microtiter plate.
• Add rabbit complement to activate cell killing if HLA
antigen is present.
• Use vital dye (e.g., eosin red) to distinguish dead (colored) vs. live cells (colorless).

Answer 7

HLA Phenotyping

• Complement-dependent cytotoxicity (CDC) test