Class: UTIs - E.coli and others

Question 1

MacConkey Agar grows what two types of organisms and shows what color

Answer 1

gram (-)

lactose fermenters = pink (lactase+)

Question 2

MC encountered enterobacteriaceae

Answer 2

e. coli

Question 3

E.coli activity on Blood agar

Answer 3

B-hemolytic

Question 4

4 virulence factors fro e. coli

Answer 4

LPS = endotoxin
Capsule (contains K-antigen )
Pili (fimbriae)
Hemolysin

Question 5

Type -1 fimbriae of E.coli bare specific for what

Answer 5

mannose on epithelial surface

Question 6

P-fimbriae of e.coli are specific for what

Answer 6

bind to Gal-Gal on P blood-group antigen

Question 7

what is most important virulence factor

Answer 7

K-antigen

Question 8

what serotype of the K-antigen is most invasive

Answer 8

K1 serotype

Question 9

most UTIs are caused by

Answer 9

e. coli

Question 10

2 complications of e.coli infx

Answer 10

neonatla meningitis

bactermia/sepsis

Question 11

how does e.coli bind uroepithelial cells

Answer 11

type-1 fimbriae

Question 12

where do e.coli bacteria live exaclty

Answer 12

invade and replicate in epithelial cells = intracellular!!!!

Question 13

2 complications of e. coli UTIs

Answer 13

cystitis

pyelonephritis

Question 14

what type of e.coli infx is the 2nd MC cuase of community acquired sepsis?

Answer 14

UTI

Question 15

E. coli is the MC cause of what nosocomial inx?

Answer 15

PNA!!!!!!!!!!!!!!! - it is #1!!!

Question 16

urine dipstick tests fro what 2 chemical

Answer 16

leukocytes esterase

nitrites

Question 17

2 other invasive infx of E.coli (other than nosocomial PNA)

Answer 17

neonatal meningitis

bacteremia/septic shock/dissemination–> PNA

Question 18

Enterobacter family is resistant to what family of ABX

Answer 18

3rd gen cephalosporins

Question 19

Enterobacter family is usually susceptible to what 2 groups of drugs

Answer 19

β-lactamase inhibitors and carbapenems

Question 20

what bacteria is a carbapenemase that merged in 2000s

Answer 20

Klebsiella

Question 21

New Delhi metallo-β-lactamase is resistant to what classes? (2)

Answer 21

all β-lactam antibiotics,

including carbapenems

Question 22

What is tx for UTI

Answer 22

Start empirically: TMP-SMX

Question 23

CRE/KPC strains are usually still susceptible to what

Answer 23

Dual therapy (ALWAYS USE 2)
tigecycline and colistin (polymyxin B)

Question 24

What is Klebsiella’s major virulence factor for invasive disease

Answer 24

– large mucoid capsule

Question 25

Klebsiella is the only bacteria of enterobacter family that causes what?

Answer 25

Community Acquired PNA

Question 26

CAPNA pts produce what that identifies Klebsiella as cause

Answer 26

thick “currant jelly” sputum.

Question 27

Klebsiella PNA common in wht 3 populations?

Answer 27

alcoholics, DM, COPD

Question 28

“swarming motility” describes what bacteria

Answer 28

Proteus

Question 29

Proteus causes what unique thing

Answer 29

staghorn calculus

Question 30

Proteus is positive for what enzyme

Answer 30

urease

Question 31

Cronobacter (Enterobacter) sakazakii is associated with what?

Answer 31

associated with contaminated powdered (dry) infant formula

Question 32

Cronobacter (Enterobacter) sakazakii causes what 3 things

Answer 32

neonatal sepsis, meningitis, necrotizing enterocolitis