Chapter 8 - Tests Flashcards
Test for PKU (6)
- newborn screening2. Guthrie Test3. Automated Technique4. Ferric Chloride5. Phenistix6. HPLC
PKU:most well known-positive: bacterial culture
Guthrie test
PKU:positive for Ferric Chloride Test
blue-green
PKU:Phenistix (+)
Blue-gray to green-gray
PKU:reference method
HPLC
Tyrosinuria Tests: (4)
- Nitrosonaphthol Test2. Millon’s Test3. Ferric Chloride test4. Phenistix
Tyrosinuria Tests:Nitrosonapthol color-specificity
orange-rednonspecific
Tyrosinuria Tests:Millon’s TestColorreagent
red precipitateMercury in conc. HNO3
Tyrosinuria Tests:Ferric Chloridecolor
transient green
Tyrosinuria Tests:Phenistixcolor
transient green
Melanuria tests:Ferric chloridecolor
gray or black precipitate
Alkaptonuria test: (4)
- Ferric chloride2. Benedict’s test3. Chromatography4. capillary electrophoresis
Alkaptonuria tests:confirmatory tests (2)
chromotographycapillary electrophoresis
Alkaptonuria tests:ferric chloridecolor
transient deep blue
Alkaptonuria tests:benedict’s or clinitest
yellow precipitate
Maple Syrup Urine Disease Test:(6)
- Modified Guthrie test2. DNPH3. Amino acid chromatography4. Ferric chloride5. Nitroso-napthol6. Acetest
Maple Syrup Urine Disease Test:DNPHcolor
yellow turbidity or precipitate
Maple Syrup Urine Disease Test:confirmatory
amino acid chromatography
Maple Syrup Urine Disease Test:ferric chloridecolor
green-gray
Maple Syrup Urine Disease Test:nitro-napthol
red
Maple Syrup Urine Disease Test:acetest
purple
Methylamolnic Acidemia screening test and color
p-nitroaniline test - emerald green
5-HydroxyIndoleacetic Acid Tests:(2)
- Ferric Chloride2. Sjoerdsma Test
5-HydroxyIndoleacetic Acid Tests:recommended specimen
first morning urine - prevent false-negative results