Chapter 8 - Tests Flashcards

2
Q

Test for PKU (6)

A
  1. newborn screening2. Guthrie Test3. Automated Technique4. Ferric Chloride5. Phenistix6. HPLC
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3
Q

PKU:most well known-positive: bacterial culture

A

Guthrie test

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4
Q

PKU:positive for Ferric Chloride Test

A

blue-green

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5
Q

PKU:Phenistix (+)

A

Blue-gray to green-gray

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6
Q

PKU:reference method

A

HPLC

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7
Q

Tyrosinuria Tests: (4)

A
  1. Nitrosonaphthol Test2. Millon’s Test3. Ferric Chloride test4. Phenistix
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8
Q

Tyrosinuria Tests:Nitrosonapthol color-specificity

A

orange-rednonspecific

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9
Q

Tyrosinuria Tests:Millon’s TestColorreagent

A

red precipitateMercury in conc. HNO3

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10
Q

Tyrosinuria Tests:Ferric Chloridecolor

A

transient green

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11
Q

Tyrosinuria Tests:Phenistixcolor

A

transient green

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12
Q

Melanuria tests:Ferric chloridecolor

A

gray or black precipitate

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13
Q

Alkaptonuria test: (4)

A
  1. Ferric chloride2. Benedict’s test3. Chromatography4. capillary electrophoresis
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14
Q

Alkaptonuria tests:confirmatory tests (2)

A

chromotographycapillary electrophoresis

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15
Q

Alkaptonuria tests:ferric chloridecolor

A

transient deep blue

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16
Q

Alkaptonuria tests:benedict’s or clinitest

A

yellow precipitate

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17
Q

Maple Syrup Urine Disease Test:(6)

A
  1. Modified Guthrie test2. DNPH3. Amino acid chromatography4. Ferric chloride5. Nitroso-napthol6. Acetest
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18
Q

Maple Syrup Urine Disease Test:DNPHcolor

A

yellow turbidity or precipitate

19
Q

Maple Syrup Urine Disease Test:confirmatory

A

amino acid chromatography

20
Q

Maple Syrup Urine Disease Test:ferric chloridecolor

A

green-gray

21
Q

Maple Syrup Urine Disease Test:nitro-napthol

A

red

22
Q

Maple Syrup Urine Disease Test:acetest

A

purple

23
Q

Methylamolnic Acidemia screening test and color

A

p-nitroaniline test - emerald green

24
Q

5-HydroxyIndoleacetic Acid Tests:(2)

A
  1. Ferric Chloride2. Sjoerdsma Test
25
Q

5-HydroxyIndoleacetic Acid Tests:recommended specimen

A

first morning urine - prevent false-negative results

26
Q

5-HydroxyIndoleacetic Acid Tests:bananas, pineappples, tomatoesmedications: phenothiazines acetanilids.

A

Diet: food not allowed - rich in serotonin

27
Q

5-HydroxyIndoleacetic Acid Tests:Ferric chloridecolor

A

blue-green

28
Q

5-HydroxyIndoleacetic Acid Tests:Sjoerdmsa test-principle-color

A

1-nitroso-2-naphthol- purple to black

29
Q

Indicanuria tests: (3)

A
  1. Obermayer’s Test2. Jaffe’s test3. Jolle’s test
30
Q

Cystinuria:- more probable stone formers of the 2 modes of inheritance

A

4 amino acid reabsorption problem

31
Q

Cystinosis Test: (2)

A
  1. cyanide-nitroprusside 2. Clinitest
32
Q

Cystinuria tests:cyanide-nitroprussidecolor

A

red-purple

33
Q

Cystinuria tests:clinitest

A

orange-red

34
Q

Homocystinuria test:(3)

A
  1. newborn screening - mod. Guthrie2. Cyanide-nitropruside 3. silver nitroprusside
35
Q

Homocystinuria test:Cyanide-nitroprussidecolorspecificity

A

red-purplenonspecific

36
Q

Homocystinuria test:sliver nitroprussidecolorspecificity

A

red purple-follow-up test after positive cyanide-nitropruside=fresh urine should be used

37
Q

Porphyria Test: (2)

A
  1. Ehrlich2. Fluorescence under UV 550-600 nm
38
Q

Porphyria Test:Fluorsecencecolor

A

violet, pink or red

39
Q

Mucopolysaccharide Disorder tests:acid-albumincolor

A

white precipitate

40
Q

Mucopolysaccharide Disorder tests:CTAB color

A

white precipitate

41
Q

Mucopolysaccharide Disorder tests:metachromatic staining spot test

A

blue dye cannot be washed

42
Q

Purine Disorder-Urine colortexture

A

Orange sand in diapers = uric acid

43
Q

Glactose and Fructose test:result- dipstick- clinitest

A

-dipstick = (-)-clinitest/copper reduction = (+)