Chapter 6: 1.0- Sediment Stain/Microscopy Flashcards
- increases overall visibility of sediments being examined
- uses bright -field microscopy
- identifying characteristics: cellular structure (nuclei, cytoplasm and inclusions)
-microscopic sediment stains
Stains:
- most frequently used stain in urinalysis
Sternheimer Malbin
Compostition of sternheimer malbin (2)
Crystal Violet and Saffranin O
Stains:
-enhancement of nuclear detail, differential WBC, RTE cell
0.5 Toludine Blue
Stains:
-enhances nuclear detail
2 % Acetic Acid
Stains:
- confirms the presence of neutral fats & TAG and cholesterol
Sudan III or Oil Red O
Stains:
- role is limited to the identification of bacterial casts, which can easily be confused with granular cast
Gram-stain
Stains:
- to confirm the presence of hemosiderin (stains blue)
Prussian Blue
Stains:
- consists of methylene blue and eosin Y in methanol; used to identify eosinophils (wright’s stain)
Hansel Stain
Stains:
-stains RBC and helps distinguish them from yeast cell which will not pick up the stain
Eosin
Stains:
-can be used to stain starch granuels and vegetable fibers a dark brown
Iodine
Type of microscope:
-Most common type performed in urinalysis lab
Brigh Field Microscopy
Type of microscope:
- works by retardation of light rays diffused
- produces halo effect around the element
Phase Contrast
Type of microscope:
- uses two polarizing filters (placed in condenser and ocular)
- aids in identification of crystals and lipids
Polarizing Microscopy
-pattern produced by lipids/fats in polarizing microscopy
Maltese cross pattern
