Chapter 6 - Microbial Nutrition and Growth Flashcards

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1
Q

Physical requirements

A

temperature, pH, water

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2
Q

Affects of temperature on microbial growth

A

membrane fluidity, protein denaturation

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3
Q

2 Physical effects of water

A

Osmotic pressure, hydrostatic pressure

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4
Q

Chemical and energy requirements

A

oxygen, nitrogen, phosphorus, sulfur, trace elements, growth factors

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5
Q

4 Toxic forms of oxygen

A

singlet oxygen, superoxide radicals, peroxide anion, hydroxyl radical

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6
Q

2 Groups of organisms based on carbon source

A

autotrophs and heterotrophs

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7
Q

Autotrophs

A

utilize inorganic source of carbon, CO2

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8
Q

Heterotrophs

A

organisms that catabolize reduced organic molecules acquired from other organisms

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9
Q

2 Groups of organisms based on energy source

A

chemotrophs and phototrophs

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10
Q

Chemotrophs

A

organisms acquiring energy from redox reactions involving inorganic and organic chemicals

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11
Q

Phototrophs

A

organisms that use light as their energy source

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12
Q

2 Groups of organisms based on electron source

A

Organotrophs and lithotrophs

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13
Q

Lithotrophs

A

organisms acquiring electrons from inorganic sources

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14
Q

Organotrophs

A

organisms acquiring electrons from organic sources

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15
Q

6 Types of general culture media

A

defined, complex, selective, differential, anaerobic, and transport

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16
Q

3 Ways of preserving cultures

A

refrigeration, deep freezing, lyophilization

17
Q

Generation time

A

time required for a bacterial cell to grow and divide

18
Q

Direct methods of measuring microbial reproduction

A

serial dilution, viable plate counts, membrane filtration, microscopic counts, electronic counters

19
Q

Indirect methods of measuring microbial growth

A

metabolic activity, dry weight, turbidity