Chapter 6 Health Surveillance and Quality Assurance Flashcards
An animal supplier’s health status report is an assurance that the animal received in the facility is healthy and free of pathogens
False
Sentinel animals should be at least what age?
8-12 weeks
Which test detects known DNA sequences characteristic for the pathogen?
Polymerase chain reaction
Who decides which specific pathogens will be surveyed for in the animal facility?
Veterinarian, with input from the research team
How soon after exposure should a sentinel animal be tested for a specific pathogen?
3 to 6 weeks after exposure
Which pinworm infection can be detected by a tape test?
Syphacia
The presence of most bacteria can be confirmed by culture on artificial media
True
The pinworm Aspiculuris tetraptera
can be detected in mice by the anal tape test
False
What does the acronym MFI stand for?
Multiplex fluorescent immunoassay
In animal rooms where microisolation cages are being used, the sentinels are housed in cages that contain what item from other cages in the room?
Mixed bedding
If an animal tests negative for a pathogen 3 weeks after arrival at the facility, which conclusion can be drawn?
The animal was free of the pathogen when shipped
Serology is the best method to detect pathogens in immunodeficient animals.
False
The sentinel program detects mostly pathogens spread by fecal-oral contamination
True
Sentinel animals are experimental animals subjected to extra testing during the course of the study.
False
The best time to monitory for microbial antibodies is when the animals are very young.
False
Which activity must be done regularly in order to maintain the water quality in automatic watering systems?
- Flush cage rack water lines
- change in-line water filters
- Sanitize water lines and connectors
Which of the following can be consequence of the introduction of insects or rodents in the facility?
- Damage to electrical cords
- Compromised experimental data
- Food contamination
Which type of feed is most likely to be used by a facility that must meet GLP standards?
Certified diets
Which of the following is a contaminant that can be found in automatic watering systems?
- Hydrocarbons
- Heavy metals
- Coliform bacteria
Any feed can be autoclaved without loss of nutrients.
False
Technology using adenosine triphosphate (ATP) test swabs uses (BLANK) to measure organic residue.
light
Which of the following is NOT provided by data obtained from microbial monitoring?
Confirmation of the validity of study results
What does the acronym “CFUs” stand for?
Colony forming units
What is contained in the paper strips used to test sanitation during autoclaving?
Bacterial spores
In addition to using tapes that indicate if the proper temperature was reached, cage washer effectiveness should be tested periodically for the presence of pathogens.
True
Which statement regarding testing water bottles for bacterial colonies is FALSE?
Enteric pathogens are unlikely to be found in used water bottles
All of the following can be increase a rodent’s susceptibility to infectious, toxic, and other harmful agents, EXCEPT which of the following?
Extra environmental enrichment
Noise level above 85 decibels may cause which of the following effects?
- Auditory effects in rodents
- A rise in blood pressure in nonhuman primates
- Auditory effects in humans
Radios should no be permitted in any room where animals are housed.
False
Levels of light photo intensity above (BLANK) foot-candles can cause retinal damage in some albino animals.
75
Which environmental parameter is reduced inside the cage with the fuse of filter-top caging?
Ventilation
Regulations and standards for USDA-regulated species are specified in which of the following?
Animal Welfare Act
Which animal would be best to use as a sentinel in a room housing a mouse colony?
4-month old mouse
Which technique is most used to detect Helicobacter spp.?
Polymerase chain reaction
Which test eliminates the need for the animal to have antibodies to the infectious agent?
PCR rodent infectious agent PRIA
Which of the following is an advantage of the adhesive plastic tape used to test the effectiveness of cage washers?
can be kept as a permanent record
Which type of bedding is required in GLP studies?
Certified
Which type of sampling can be used in place of bedding sentinels?
Environmental dust
Which of the following is a main factor that determines the number of sentinels used?
expected morbidity of the agents being monitored
Which of the following applies to certified feed?
The concentration of contaminants is below maximum acceptable levels
One way to monitor the effectiveness of the cage washing system is to use adhesive plastic tape that changes color when exposed to a temperature of:
82 plus/minus 1 degree Celsius
What is a common method used to detect exposure to viral pathogens?
Enzyme-linked immunosorbant assay (ELISA)
Which type of bedding is required in GLP studies?
Certified
Regulations and standards for USDA- regulated species are specified in which of the following?
Animal Welfare Act
Which of the following applies to certified feeds?
The concentration of contaminants is below maximum acceptable levels.
