Chapter 5: Laboratory Animal Facility Design & Environmental Management

Question 1

What is the purpose of a two-corridor floor plan?

Answer 1

To separate clean incoming items from soiled outgoing items

Question 2

These features apply to what type of facility:
PPE required for entry
Room air pressure positive relative to corridor

Answer 2

Bioexclusion

Question 3

These features apply to what type of facility:
PPE required for entry
Anteroom used for dining PPE
procedures follow the BMBL
Eye or respiratory protection often required
May be required to shower upon exiting
Room air pressure negative relative to corridor

Answer 3

Biocontainment

Question 4

List 4 procedures that support animal biosecurity

Answer 4

1- acquiring SPF animals
2- servicing colony animals prior to animals in quarantine or isolation
3- pest control program
4- health surveillance program

Question 5

Convert an anemometer reading of 825 cubic feet per minute to cubic feet per hour

Answer 5

825 cubic feet/minute X 60 min/hour = 49,500 cubic feet/hour

Question 6

Determine the volume of the room:25 x 20 x 15 foot room

Answer 6

V = ht x w x l

25 ft X 20 ft X 15 ft = 7500 cubic feet

Question 7

Find the air changes per hour for room: CFH 49,500 room volume 75000

Answer 7

49,500 CFH / 7500 cubic feet = 6.6 changes/hr

Question 8

What is the desired number of air changes per hour?

Answer 8

10

Question 9

Where does the air move in a positive pressure ventilation in the room when the door is opened

Answer 9

Out of the room

Question 10

Where does the air move in a negative pressure ventilation in the room when the door is opened

Answer 10

Into the room

Question 11

True or False

The number of air changes in a room per hour should never exceed five

Answer 11

False

It should be 10-15 fresh air changes per hour

Question 12

True or False

Sterilization is best accomplished by using only hot water from the faucet

Answer 12

False

This is sanitizer

Question 13

True or False
All disinfectants designed for use on hard surfaces are regulated under the Federal Insecticide, Fungicide, and Rodenticide Act

Answer 13

True

Question 14

True or False

Phenolics are extremely hazardous to several animal species, such as cats and amphibians

Answer 14

True

Question 15

Most laboratory animals can tolerate a temperature range of _________C or ________F

Answer 15

21.1-24.5C

70-76F

Question 16

The relative humidity should be in the range of ________ for most mammalian species

Answer 16

30-70%

Question 17

An instrument that measures humidity

Answer 17

Hygrometer

Question 18

A floor plan consisting of a central corridor with no separation of clean/dirty traffic

Answer 18

Single-corridor

Question 19

A type of facility designed to keep pathogens from leaving the room

Answer 19

Biocontainment

Question 20

A type of thermometer where the bulb is protected from moisture

Answer 20

Dry-bulb

Question 21

This system provides a continuous uniform, unidirectional flow of filtered air

Answer 21

Laminar airflow

Question 22

Resting boards or perches in cages are examples of this type of enrichment

Answer 22

Structural

Question 23

A list of specific organisms on a label for which a chemical agent was tested

Answer 23

Spectrum of activity

Question 24

_____________ is an indication of the amount of water present in air and is expressed as a percentage of the maximum amount of water the air is able to hold at the current temperature

Answer 24

Relative humidity

Question 25

Why is traffic flow important in the design of a laboratory animal facility?

Answer 25

To prevent cross-contamination

Question 26

A resting board is what type of enrichment

Answer 26

Structural enrichment

Question 27

A puzzle feeder is what type of enrichment

Answer 27

Sensory/motor stimulation

Question 28

What type of enrichment is walking on leash with handler

Answer 28

Social interaction with humans

Question 29

What type of enrichment is balls on chains

Answer 29

Sensory/motor stimulation

Question 30

Reduces but does not necessarily eliminate microorganisms

Answer 30

Sanitation

Question 31

Destroys or inactivates infectious fungi and bacteria, but not necessarily their spores

Answer 31

Disinfection

Question 32

Kills all forms of microbial life and their spores

Answer 32

Sterilization

Question 33

Measure of dissolved minerals in water

Answer 33

Hardness

Question 34

Common household bleach

Answer 34

Hypochlorite

Question 35

Iodine-containing compound

Answer 35

Iodophors

Question 36

How long a disinfectant remains on a surface

Answer 36

Contact time

Question 37

Organisms against which a product was tested

Answer 37

Spectrum of activity

Question 38

Measure of acidity or alkalinity

Answer 38

pH

Question 39

Uses pressurized steam to sterilize

Answer 39

Autoclave

Question 40

When decontaminating or sterilizing, what 3 factors of a chemical agent will determine whether the method will be successful?

Answer 40

1- distribution
2- penetration
3- contact time

Question 41

True or False

Load up the autoclave so there is no free space between items

Answer 41

False

Space between items is necessary for steam to flow uniformly around them

Question 42

True or False

Bottles of alcohol can be uroclaved if the caps are loosened

Answer 42

False

Alcohol is flammable

Question 43

Bottles of liquids should be no more than 2/3 full

Answer 43

True

Question 44

True or False

When the autoclaving cycle ends, don gloves and immediately remove items from the autoclave

Answer 44

False

Wait 5 minutes for handling no liquid items and 10 minutes for bottles of liquids