Chapter 5: Laboratory Animal Facility Design & Environmental Management Flashcards
What is the purpose of a two-corridor floor plan?
To separate clean incoming items from soiled outgoing items
These features apply to what type of facility:
PPE required for entry
Room air pressure positive relative to corridor
Bioexclusion
These features apply to what type of facility:
PPE required for entry
Anteroom used for dining PPE
procedures follow the BMBL
Eye or respiratory protection often required
May be required to shower upon exiting
Room air pressure negative relative to corridor
Biocontainment
List 4 procedures that support animal biosecurity
1- acquiring SPF animals
2- servicing colony animals prior to animals in quarantine or isolation
3- pest control program
4- health surveillance program
Convert an anemometer reading of 825 cubic feet per minute to cubic feet per hour
825 cubic feet/minute X 60 min/hour = 49,500 cubic feet/hour
Determine the volume of the room:25 x 20 x 15 foot room
V = ht x w x l
25 ft X 20 ft X 15 ft = 7500 cubic feet
Find the air changes per hour for room: CFH 49,500 room volume 75000
49,500 CFH / 7500 cubic feet = 6.6 changes/hr
What is the desired number of air changes per hour?
10
Where does the air move in a positive pressure ventilation in the room when the door is opened
Out of the room
Where does the air move in a negative pressure ventilation in the room when the door is opened
Into the room
True or False
The number of air changes in a room per hour should never exceed five
False
It should be 10-15 fresh air changes per hour
True or False
Sterilization is best accomplished by using only hot water from the faucet
False
This is sanitizer
True or False
All disinfectants designed for use on hard surfaces are regulated under the Federal Insecticide, Fungicide, and Rodenticide Act
True
True or False
Phenolics are extremely hazardous to several animal species, such as cats and amphibians
True
Most laboratory animals can tolerate a temperature range of _________C or ________F
21.1-24.5C
70-76F
The relative humidity should be in the range of ________ for most mammalian species
30-70%
An instrument that measures humidity
Hygrometer
A floor plan consisting of a central corridor with no separation of clean/dirty traffic
Single-corridor
A type of facility designed to keep pathogens from leaving the room
Biocontainment
A type of thermometer where the bulb is protected from moisture
Dry-bulb
This system provides a continuous uniform, unidirectional flow of filtered air
Laminar airflow
Resting boards or perches in cages are examples of this type of enrichment
Structural
A list of specific organisms on a label for which a chemical agent was tested
Spectrum of activity
_____________ is an indication of the amount of water present in air and is expressed as a percentage of the maximum amount of water the air is able to hold at the current temperature
Relative humidity
Why is traffic flow important in the design of a laboratory animal facility?
To prevent cross-contamination
A resting board is what type of enrichment
Structural enrichment
A puzzle feeder is what type of enrichment
Sensory/motor stimulation
What type of enrichment is walking on leash with handler
Social interaction with humans
What type of enrichment is balls on chains
Sensory/motor stimulation
Reduces but does not necessarily eliminate microorganisms
Sanitation
Destroys or inactivates infectious fungi and bacteria, but not necessarily their spores
Disinfection
Kills all forms of microbial life and their spores
Sterilization
Measure of dissolved minerals in water
Hardness
Common household bleach
Hypochlorite
Iodine-containing compound
Iodophors
How long a disinfectant remains on a surface
Contact time
Organisms against which a product was tested
Spectrum of activity
Measure of acidity or alkalinity
pH
Uses pressurized steam to sterilize
Autoclave
When decontaminating or sterilizing, what 3 factors of a chemical agent will determine whether the method will be successful?
1- distribution
2- penetration
3- contact time
True or False
Load up the autoclave so there is no free space between items
False
Space between items is necessary for steam to flow uniformly around them
True or False
Bottles of alcohol can be uroclaved if the caps are loosened
False
Alcohol is flammable
Bottles of liquids should be no more than 2/3 full
True
True or False
When the autoclaving cycle ends, don gloves and immediately remove items from the autoclave
False
Wait 5 minutes for handling no liquid items and 10 minutes for bottles of liquids
