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LAT > Unit Two Exam: Ch 5 & 6 > Flashcards

Unit Two Exam: Ch 5 & 6 Flashcards

1
Q 
Which method is NOT a method of sterilization?
1- moist heat (steam)
2- dry heat
3- radiation
4- isopropyl alcohol
A

D- isopropyl alcohol

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2
Q

HEPA filters are only efficient in removing up to _____ of particulate matter

A

99.7%

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3
Q

Hw is ammonia gas produced in soiled cage bedding?

A

From bacterial metabolism of urine products

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4
Q

An improper light cycle and excessive noise levels have been documented to have negative effects on ______ in rodents.

A

Reproduction

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5
Q

Which sequence lists d contamination procedures from greatest to least effect in eliminating microbes?

A

Sterilization, disinfection, sanitation

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6
Q

Which of the following is a method of monitoring the effectiveness of sanitation and sterilization?
A- examining the area under ultraviolet light
B- testing for pH stability
C- sampling the area using RODAC plates
D- checking the HEPA filters

A

C- sampling the area using RODAC plates

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7
Q

What is a fomite?

A

An inanimate object capable of being contaminated with infectious organisms

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8
Q

Which two speci s are required by law to have enrichment plans in a facility?

A

Dogs, nonhuman primates

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9
Q

Some microorganisms survive dry heat sterilization by producing heat-resistant _______

A

Spores

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10
Q

Which type of floor plan has NO separation of traffic by clean and dirty status?

A

Single-corridor floor plan

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11
Q

What is the general recommendation for air changes per hour in an animal room?

A

10-15

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12
Q

What is the meaning of a disinfectant’s “spectrum of activity”?

A

The specific organisms the product was tested against

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13
Q

What is the primary no chemical means to sterilize supplies in an animal facility?

A

Autoclave

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14
Q

How do biological indicators demonstrate ineffective sterilization?

A

The indicator organism grows in culture

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15
Q

What is done to render ethylene oxide nonflammable?

A

It is combined with carbon dioxide or nitrogen

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16
Q

What does the prefix “hydro” mean?

A

Humidity

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17
Q

What is the objective of a sanitation program?

A

To reduce microbes to a level where there is minimal possibility of introducing an infection f of the environment into the laboratory

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18
Q

What does a temperature indicator that has change color indicate?

A

The correct temperature was reached in the location of the indicator

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19
Q

What is the function of the autoclave jacket?

A

It preheats the chamber and provides heat for drying

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20
Q

What term identifies facilities that prevent entry or exit of microorganisms?

A

Barrier facilities

21
Q

Why should chemical residues not be allowed to remain on equipment or surfaces?

A

To prevent them from having a toxic effect on animals

22
Q

What is the correct name for the acronym SPF?

A

Specific pathogen free

23
Q

What measures should be taken to prevent allergen exposure while working in an animal facility?

A

Wear personal protective equipment

24
Q

How many general classes of biological safety cabinets are there?

A

3

25
Q

At which biosafety level would highly infectious agents with no known effective treatment be found?

A

4

26
Q

A room will be at _______ pressure if the air flow into the room is greater than the exhaust flow out.

A

Positive

27
Q

When should singly housing a social species be considered?

A

When scientifically justified

28
Q 
What is one reason that the use of alcohols is discouraged?
A- they stain metal surfaces
B- they stay in the air for a long time
C- they sensitize personnel to allergens
D- they are flammable
A

D- they are flammable

29
Q 
The circulation of \_\_\_\_\_\_\_\_ has a major impact on the micrological status of animals.
A- people
B- animals
C- equipment
D- all of the above
A

D- all of the above

30
Q

What do individual ventilated cages control?

A

Air quality

31
Q

What is the physical form of most chemical sterilizers?

A

Gas

32
Q 
Which of the following is a way to monitor the effectiveness of sanitation and sterilization?
A- visual inspection
B- biological indicators
C- microbial swabs
D- all of the above
A

D- all of the above

33
Q

Which tank system uses the greatest amount of water?

A

Flow-through

34
Q

What can be added to an aquatic system to raise the pH?

A

Sodium bicarbonate

35
Q

What will happen to an aquatic species if the water in the system has too few ions?

A

Water will flow into the animal

36
Q

Which is a measure of both calcium and magnesium ions in water?

A

Total hardness

37
Q

Which are byproducts of ammonia breakdown?

A

Nitrites and nitrates

38
Q 
Which filter can be used in recirculating systems to remove waste products from the circulating water?
A- biological
B- mechanical
C- chemical
D- all of the above
A

D- all of the above

39
Q

Which is NOT an advantage or using a recirculating tank system?
A- discourages algae growth
B- keeps nitrogen levels down
C- minimal risk of tank-to-tank contamination
D- uses less water than a flow-through system

A

C- minimal risk of tank-to-tank contamination

40
Q

Which feature makes amphibians and fish particularly susceptible to toxins?

A

Permeable skin

41
Q

What is contained in some detergents that is highly toxic to amphibians so these products should never be used to clean amphibian housing?

A

Phenolics

42
Q

Which is a standard method for dechlorinating water?
A- aging the water
B- saturating with carbon dioxide
C- adding calcium carbonate
D- passing water through deactivated carbon filters

A

A- aging the water

43
Q

Fish can develop what condition if the water becomes oversaturated with dissolved gases?

A

Gas bubble disease

44
Q 
Which can be used as a biological filter for an aquatic tank system?
A- gravel
B- sponge filter
C- activated carbon
D- ultraviolet light
A

A- gravel

45
Q

Which criteria must be met for ultraviolet light to kill microorganisms?

A

Water must flow at the proper rate

46
Q

What can happen if a frog placed into a tank with high ion concentrations in the tank water?

A

The frog can lose water from his body

47
Q 
In what types of environments can amphibians be found?
A- aquatic
B- semi aquatic 
C- terrestrial
D- all of the above
A

D- all of the above

48
Q

What is the correct range of water temperature for raising Zebrafish?

A

24-28C

49
Q

Which factor most affects the amount of dissolved oxygen in tank water?

A

Temperature of the water

LAT (23 decks)

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