Unit Two Exam: Ch 5 & 6 Flashcards
Which method is NOT a method of sterilization? 1- moist heat (steam) 2- dry heat 3- radiation 4- isopropyl alcohol
D- isopropyl alcohol
HEPA filters are only efficient in removing up to _____ of particulate matter
99.7%
Hw is ammonia gas produced in soiled cage bedding?
From bacterial metabolism of urine products
An improper light cycle and excessive noise levels have been documented to have negative effects on ______ in rodents.
Reproduction
Which sequence lists d contamination procedures from greatest to least effect in eliminating microbes?
Sterilization, disinfection, sanitation
Which of the following is a method of monitoring the effectiveness of sanitation and sterilization?
A- examining the area under ultraviolet light
B- testing for pH stability
C- sampling the area using RODAC plates
D- checking the HEPA filters
C- sampling the area using RODAC plates
What is a fomite?
An inanimate object capable of being contaminated with infectious organisms
Which two speci s are required by law to have enrichment plans in a facility?
Dogs, nonhuman primates
Some microorganisms survive dry heat sterilization by producing heat-resistant _______
Spores
Which type of floor plan has NO separation of traffic by clean and dirty status?
Single-corridor floor plan
What is the general recommendation for air changes per hour in an animal room?
10-15
What is the meaning of a disinfectant’s “spectrum of activity”?
The specific organisms the product was tested against
What is the primary no chemical means to sterilize supplies in an animal facility?
Autoclave
How do biological indicators demonstrate ineffective sterilization?
The indicator organism grows in culture
What is done to render ethylene oxide nonflammable?
It is combined with carbon dioxide or nitrogen
What does the prefix “hydro” mean?
Humidity
What is the objective of a sanitation program?
To reduce microbes to a level where there is minimal possibility of introducing an infection f of the environment into the laboratory
What does a temperature indicator that has change color indicate?
The correct temperature was reached in the location of the indicator
What is the function of the autoclave jacket?
It preheats the chamber and provides heat for drying
What term identifies facilities that prevent entry or exit of microorganisms?
Barrier facilities
Why should chemical residues not be allowed to remain on equipment or surfaces?
To prevent them from having a toxic effect on animals
What is the correct name for the acronym SPF?
Specific pathogen free
What measures should be taken to prevent allergen exposure while working in an animal facility?
Wear personal protective equipment
How many general classes of biological safety cabinets are there?
3
At which biosafety level would highly infectious agents with no known effective treatment be found?
4
A room will be at _______ pressure if the air flow into the room is greater than the exhaust flow out.
Positive
When should singly housing a social species be considered?
When scientifically justified
What is one reason that the use of alcohols is discouraged? A- they stain metal surfaces B- they stay in the air for a long time C- they sensitize personnel to allergens D- they are flammable
D- they are flammable
The circulation of \_\_\_\_\_\_\_\_ has a major impact on the micrological status of animals. A- people B- animals C- equipment D- all of the above
D- all of the above
What do individual ventilated cages control?
Air quality
What is the physical form of most chemical sterilizers?
Gas
Which of the following is a way to monitor the effectiveness of sanitation and sterilization? A- visual inspection B- biological indicators C- microbial swabs D- all of the above
D- all of the above
Which tank system uses the greatest amount of water?
Flow-through
What can be added to an aquatic system to raise the pH?
Sodium bicarbonate
What will happen to an aquatic species if the water in the system has too few ions?
Water will flow into the animal
Which is a measure of both calcium and magnesium ions in water?
Total hardness
Which are byproducts of ammonia breakdown?
Nitrites and nitrates
Which filter can be used in recirculating systems to remove waste products from the circulating water? A- biological B- mechanical C- chemical D- all of the above
D- all of the above
Which is NOT an advantage or using a recirculating tank system?
A- discourages algae growth
B- keeps nitrogen levels down
C- minimal risk of tank-to-tank contamination
D- uses less water than a flow-through system
C- minimal risk of tank-to-tank contamination
Which feature makes amphibians and fish particularly susceptible to toxins?
Permeable skin
What is contained in some detergents that is highly toxic to amphibians so these products should never be used to clean amphibian housing?
Phenolics
Which is a standard method for dechlorinating water?
A- aging the water
B- saturating with carbon dioxide
C- adding calcium carbonate
D- passing water through deactivated carbon filters
A- aging the water
Fish can develop what condition if the water becomes oversaturated with dissolved gases?
Gas bubble disease
Which can be used as a biological filter for an aquatic tank system? A- gravel B- sponge filter C- activated carbon D- ultraviolet light
A- gravel
Which criteria must be met for ultraviolet light to kill microorganisms?
Water must flow at the proper rate
What can happen if a frog placed into a tank with high ion concentrations in the tank water?
The frog can lose water from his body
In what types of environments can amphibians be found? A- aquatic B- semi aquatic C- terrestrial D- all of the above
D- all of the above
What is the correct range of water temperature for raising Zebrafish?
24-28C
Which factor most affects the amount of dissolved oxygen in tank water?
Temperature of the water
