chapter 5-cellular biology Flashcards

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1
Q

what is the cell theory

A

1) all living things are composed of cells
2) the cell is the basic functional unit of life
3) the chemical reactions of life take place inside the cell
4) cells arise only from pre-existing cells
5) cells carry genetic information in the form of DNA. this genetic material is passed from parent cell to daughter cell

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2
Q

what are the 6 kingdoms

A

1) bacteria
2) Archaea
3) Protista
4) Fungi
5) Plantae
6) Animalia

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3
Q

Cell membrane function

A

1) encloses the cell and exhibits selective permeability
2) regulates the passage of materials into and out of the cell
3) consists of a phospholipid bilayer with proteins embedded throughout.

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4
Q

fluid mosaic model

A
  • cell membrane consists of a phospholipid bilayer with proteins embedded throughout.
  • The lipids and many of the proteins can move freely within the membrane
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5
Q

structure of phospholipid

A
  • long, nonpolar, hydrophobic, fatty chains of carbon and hydrogen face each other
  • phosphorus containing, polar, hydrophobic heads facing outwards
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6
Q

what kind of structures are readily permeable in the plasma membrane

A
  • small, hydrophobic, non polar
    AND
  • small polar
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7
Q

What kind of particles cross the membrane though protein channels

A

small charged particles

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8
Q

what kind of particles cross the membrane through carrier proteins

A

charged ions and larger charged mc

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9
Q

what are the dense structures in the nucleus and what occurs there

A

nucleolus and rRNA is synthesized

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10
Q

what are histones and how does DNA complex with it during DNA replication and transcription

A
  • histones are structural proteins with DNA wound around it
  • DNA replication: DNA tightly winds around the histone compacting chromatin into chromosome
  • transcription: DNA unwinds itself from the histone to allow transcription to occur
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11
Q

what is chromatin

A

loose DNA

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12
Q

nucleoid

A

in prokaryotes

- contains the circular chromosomes

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13
Q

what is the role of ribosomes and what are they made of

A
  • role: protein production

- 2 rRNA sequences called ribosomal subunits

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14
Q

in Eukaryotes where are ribosomes synthesized and function

A

synthesized in the nucleolus and function in cytoplasm

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15
Q

in prokaryotes where are ribosomes synthesized and function

A

synthesized and function in cytoplasm

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16
Q

what is the ER

A

is a network of membrane-enclosed spaces involved in the transport of materials throughout the cell particularly those materials designed to be secreted by the cell

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17
Q

role of Rough ER

A

contains ribosomes and plays an important roll in the production of proteins

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18
Q

role of Smooth ER

A

involved with metabolism and production of lipids

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19
Q

role of golgi

A

-Receives vesicles and their contents from the smooth ER and then modifies them (ex. glycosylation), repackages them into vesicles and distributes the to the cell surface for exocytosis

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20
Q

Role of mitochondria

A

site of aerobic respiration within the cell, responsible for the conversion of sugars, fats, and other sources of fuel into usable energy ( specifically ATP)
-Contains its own genome ( which resembles cellular bacterial chromosomes)

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21
Q

structure of mitochondria

A
  • Outer membrane: fomrs barrier with the cytosol
  • Inner membrane: is folded into cristae and contains enzymes for the ETC
  • Intermembrane space ( between the 2 membranes
  • Matrix: within the inner membrane: contains the genome
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22
Q

how does the mitochondria DNA divide

A

binary fission

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23
Q

where does the cells metabolic activity occur

A

in the cytoplasms

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24
Q

by what does transport within the cells cytoplasm occur

A

cyclosis: streaming movement within the cell

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25
Q

what are Vacuoles and Vesicles and their role

A

membrane bound sacs involved in the transport and storage of materials that are ingested secreted processed or digested by the cell

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26
Q

centrioles

A

composed of MT and are involved in spindle organization during cell division

  • Not membrane bound
  • Animal cells have a pair of centrioles oriented at R angles to each other that lie in a region of the cell called the centrosome
  • Plant cells do not have centrioles
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27
Q

what is a centrosome

A

organizes MT and helps regulate the progression of the cell cycle

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28
Q

function of lysosomes

A

membrane bound vesicles that break down material ingested by the cell
- contain hydrolytic enzymes

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29
Q

An injured cell or dying cell may self-destruct by rupturing the lysosome membrane and releasing its hydrolytic enzymes called

A

autolysis

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30
Q

function of cytoskeleton

A

supports the cell, maintains its shape and aids in cell motility

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31
Q

what is the cytoskeleton composed of

A

MT, microfilaments, intermediate filaments

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32
Q

what are microtubules

A
  • hallow rods made up of polymerized tubulin that radiate throughout the fell and provide it with support
  • They provide a framework for organelle movement within the cell
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33
Q

what are centriole function and what are they composed of

A
  • they direct the separation of chromosomes during cell division
  • composed of MT
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34
Q

what are cilia and flagella

A

they are specialized arrangements of MT that extend from certain cells and are involved in cell memory and cytoplasmic movement

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35
Q

what are Microfilaments

A

are solid rods of actin which are important in cell movement as well as support
- Move materials across the PM

36
Q

what kind of proteins are intermediate filaments + ex

A

diverse group of filament proteins ex keratin

37
Q

function of intermediate filament

A

structural backbone of the cell as they are able to withstand a tremendous amount of tension ( make cell structure more rigid) AND
help anchor organelles to their respective place in the cell

38
Q

what is simple diffusion

A

is the net movement of dissolved particles down their concentration gradient (high to lower [ ])
- passive process

39
Q

what is osmosis

A

simple diffusion of water from lower solute [ ] to a region of higher solute [ ]

40
Q

what is a hypertonic medium to the cell and what occurs

A

the medium outside the cell has higher solute [ ] and water flows out
- plasmolysis occurs

41
Q

what is plasmolysis

A

shrivelling of the cell

42
Q

what is a hypotonic medium to the cell and what occurs

A

when the extracellular environment is less concentrated than the cytoplasm of the cell and water flows into the cell
- the cell lyse (bursts)

43
Q

what is when a cell is isotonic to the environment and what happens

A

when the extracellular environment has the same concentration of solutes as the cell cytoplasm
- water flows back and forth in equal amounts across the cell membrane

44
Q

during osmosis water moves towards___equilibrium

A

isotonic

45
Q

what is facilitated diffusion

A

the net movement of dissolved particles down their concentration gradient through special channels or carrier proteins in the cell membrane
- no energy required

46
Q

what is active transport

A
  • the net movement of dissolved particles against their [ ] gradient with the help of transport proteins
  • requires energy
47
Q

what are the carrier proteins

A
  • Symporters: move 2 or more ions or mc in the same direction across the membrane
  • Antiporters: exchange one or more ions ( or mc) for another ion or mc acorss the membrane
  • Pumps: energy-dependent carriers ( require ATP) ex Na/K pump
48
Q

what is endocytosis

A

a process in which the cell membrane invaginates, forming a vesicel that contains extracellular medium
-allows the cell to bring large volumes of extracellular material inside the cell

49
Q

types of endocytosis

A
  • pinocytosis: ingestion of fluids or small particles
  • phagocytosis: engulfing of large particles (particles may bind to receptors on the cell membrane, triggering endocytosis)
50
Q

what is exocytosis

A
  • a vesicle within the cell fuses with the cell membrane and releases a large volume of content to the outside
  • fusion of the vesicle with the cell membrane can play an important role in cell growth and intercellular signaling ex. NT that act as signals to neighboring cells
51
Q

what is cell division

A

: cell doubles its organelles and cytoplasm, replicates its DNA and divides in 2

52
Q

what is the period of growth and chromosome replication in cell cycle

A

interphase

53
Q

how long does a cell spend in interphase

A

90% of its life

54
Q

after replication the chromosome consists of 2 identical sister chromatids held together by what

A

a central regions called centromere

55
Q

how does the DNA appear during interphase

A

individual chromosomes are not visible but the DNA is uncoiled and called chromatin

56
Q

what are the 3 parts of interphase

A

1) G1: initiation of interphase
- active growth phase, cell size increase and synthesizes of proteins occurs
- varies in size
2) S: DNA synthesis
3) G2: cell prepares to divide, grows and synthesizes proteins

57
Q

what type of cells go through mitosis

A

somatic cells

58
Q

what is mitosis

A

division and distribution of the cells DNA to its 2 daughter cells such that eachc ell receives a complete copy of the original genome

59
Q

what is karyokinesis

A

nuclear division

60
Q

what is cytokinesis

A

cell division

61
Q

what occurs in prophase

A
  • cell prepares for karyokinesis
    1) chromatin is condensed into chromosomes
    2) nuclear membrane dissolves
    3) centriole pairs separate and move towards opposite poles of the cells
62
Q

what occurs in metaphase

A

1) centrioles are at opposite poles and form spindle fibres to anchor themselves
2) more spindle fibres radiate outwards and attach to each chromatid at the kinetochore
3) the spindle finer aligns the chromosome along the metaphase plate

63
Q

what is a kinetochore

A

a protein located on the centromere of the chromosome

64
Q

what occurs during anaphase

A

1) centromeres split so that ach chromatid has its own distinct centromere
2) sister chromatids are pulled towards opposite poles by shortening of the spindle fibers
3) separation of sister chromatid of each chromosome

65
Q

what occurs during telophase

A

1) spindle apparatus disappears
2) a nuclear membrane forms around each set of newly formed chromosomes
3) each nucleus contains the same # of chromosomes ( diploid number) as the original nucleus
4) chromosomes uncoil, resuming their interphase form
5) towards the end: cytoplasm divides into 2 daughter cells each with a complete nucleus and its own set of organelles

66
Q

what occurs during cytokinesis

A

1) animal cells: cleavage furrow forms: cell membrane indents along the equator of the cell and separates into 2 nuclei
2) plant cells: cell plate forms between the 2 nuclei splitting the plant in half and allows the cell to divide

67
Q

how is mitosis different than meiosis

A

2 parents are involved (fusion of 2 gametes) and it produces haploid number instead of preserving its diploid number

68
Q

what is meiosis

A

the process by which these sex cells are produced

69
Q

what are sex cells

A

gametes

70
Q

what is ploidy

A

how many chromosomes an organism has in a homologous set

71
Q

what is a homologous set

A

chromosomes that share structure and gene location but can have different alleles

72
Q

what does the first meiotic division yield

A

2 haploid daughter cells ( separates the pair of homologues chromosomes ( not chromatids)

73
Q

what occurs during prophase 1

A

1) chromatin condenses into chromosomes
2) spindle apparatus forms
3) nucleoli/nuclear membrane disappears
4) crossing over occurs: the genetic exchange between chromatids of homologous chromosomes and its vital for a species as it increases genetic diversity

74
Q

crossing over steps

A

1) homologous chromosomes come together and intertwine called synapsis forming a tetrad ( 4 sister chromatids)
- where the sister chromatids interact is called chiasmata
2) once synapsis begins chromatids of homologues chromosomes break at corresponding point and exchange equivalent pieces of DNA

75
Q

what is the process synapsis

A

meiosis 1

- homologous chromosomes come together and intertwine

76
Q

what occurs during metaphase 1

A

-homologous pairs ( tetrads) align at the equatorial plane and each pair attaches to a separate spindle fiber at the kinetochore

77
Q

what occurs during anaphase 1

A

1) homologous pairs separate and are pulled to opposite poles of the cell called disjunction (accounts for the Medelian law of segregation)
2) during disjunction: each chromosome of paternal orgin separates ( or disjoins) from its homologue of maternal origin and their chromosomes end up in either daughter cell
3) each daughter cell will have a unique pool of genes from a random mixture of maternal and paternal origin

78
Q

what is nondisjunction and when does it occur

A

when cells don’t separate appropriately during meiosis resulting in the daughter cell having an incorrect number of chromosomes
- anaphase 1

79
Q

what occurs using telophase 1

A

1) Nuclear membrane forms around each new nucleus thereby forming 2 haploid cells in which each chromosome has 2 sister chromatids these cells are intermediate daughter cells

80
Q

similarities and differences between the second meiotic division and mitosis

A

similar to mitosis bc sister chromatids are separated BUT meiosis both parent (intermediate daughter cells) and daughter cells are haploid not diploid

81
Q

what occurs during prophase 2

A

1) Nuclear envelope dissolves
2) nucleoli disappear
3) the centrioles migrate to opposite poles
4) spindle apparatus begins to form

82
Q

what occurs during metaphase 2

A

Chromosomes line up on the metaphase plate

83
Q

what occurs during anaphase 2

A

1) Sister chromatids of the chromosomes are separated and are pulled apart by shortening spindle fibers
2) Each separated sister chromatid is now considered a chromosome itself

84
Q

what occurs during telophase 2

A

1) Nuclear envelope forms around each new set of chromosomes

2) Cytokinesis follows and 2 haploid daughter cells are formed per intermediate daughter cells= completion of meiosis 2

85
Q

how many daughter cells are produced per gametocyte

A

up to 4 haploid daughter cells