Chapter 4 (21) Flashcards
replication
when DNA is copied before every cell division
transcription to RNA
when DNA is copied for instructions to be activated
DNA replication steps
- enzyme “unzips” H-bonds, separating strands
- new nucleotides are added by a second enzyme
- complementary base pairing ( A&T, C&G)
- proofreading & error correction
- Final enzyme seals breaks in backbone
- mutation if any change in sequence between original and final
what are the 4 categories of RNA
messenger, ribosomal, transfer, small RNAs
messenger RNA
carries copy of DNA template for translation into protein
ribosomal RNA
combines with proteins to make ribosomes
transfer RNA
transfers amino acids to ribosomes
small RNA
assist with making mRNA and rRNA
Transcription
making of mRNA in nucleus
steps of transcription
- enzyme unzips section of DNA
- complementary base pairing (but with U) from just one strand
- joining of nucleotides along P backbone
- Processing to mature
removing non-coding parts (introns) & leave only coding parts (exons)
add cap and tail to ends
Translation
turning mRNA into amino acid sequence in cytoplasm
translation steps
- mRNA has code-3 nucleotides are CODON for one amino acid.
- tRNA has anticodon to complement codon and carries one amino acid
- tRNA has anticodon to complement codon and carries one amino acid
- ribosome reads mRNA and has binding sites where codons and anticodons match up
p-site holds tRNA with polypeptide chain, A-site holds tRNA with next amino acid, peptide bond forms, P-site tRNA detaches, A-site tRNA moves over to P-site, repeat until termination signal
one mRNA can have many
ribosomes reading it at once
polypeptide chains must go through
further processing & folding to make working enzymes
what are the 4 types of tissues
connective, muscle, nervous, epithelial
connective tissue
“glue” of body
cells mixed with extracellular matrix of proteins and ground substances
protein fibers: collagen, elastic, reticular
ground substances: solid, fluid, semi-like
three types of connective tissue
fibrous, supportive, fluid