Chapter 3 Flashcards
The procedure for staining cells is _, also known as the _
1)smear-spread on slide
2) stick-adhere to slide
3)stain-dye
the 3 S’s
The two types of stains are
1)positive (stains cells)
2)negative(stains background)
A simple stain uses _ dye(s)
1
A differential stain uses _ dye(s)
2
There are _ types of differential stains. They are _
4
1)gram stain
2)acid-fast stain
3)endospore stain
4)structural stain
In a gram stain, the purple are ___ cells
gram-positive
In a gram stain, the red are ___ cells
gram-negative
In an acid-fast stain, the red are __ cells, and the blue are ____ cells
positive
negative
In an endospore stain the green are ___ cells and the red are ___ cells
endospore
vegetative
In a structural stain(this enhances cell detail), a subtype is capsule and a negative stain stains the ____
background
There are __ types of media which are ____
three
1)physical state
2)chemical composition
3)funciton
A physical state is a ___ without ____ like ___, or it is a _____ with agar
liquid; agar; broth
semi(solid)
Under chemical composition, ___ requires a specific amount whereas ___ is less defined (e.g., ____)
chemically defined
complex
chemoheterotrophs
There are ___ functions of media which are __
5
1)general purpose(broad level of microbes to grow with no requirement (e.g., nutrient agar)
2)enriched (increase number of microbes to detectable levels)
3)reducing (no oxygen and they are obligate anaerobes
4)selective (suppresses unwanted microbes and encourages desired
5) differential (differentiate bacteria on the same medium)
Spread, Stick, Stain
Size, Shape, Arrangement
+ stain cells, - stains background
B
B
C
