Chapter 13- Molecular basis of inheritance Flashcards
Trasnformation
A change in genotype and phenotype due to the assimilation of external DNA
DNA Replication
when DNA is copied using RNA
Phages
Viruses that infect bacteria
virus
DNA enclosed by a protective coat if protein
Double Helix
the Shape that is defined as the shape of DNA
Antiparallel
when the subunits of DNA run in opposite directions
semiconservative Model
model of replication in which the the strands split and each of the replicate DNA have a strand of the parent
origins of Replicaiton
short stretches of DNA having a specific sequence of nucleotides, proteins find this sequence and attach to start replication
replication fork
y shaped region of DNA that are being unwound by proteins
helicases
enzymes that untwist the two parental strands and make them available as parental strands
single strand binding proteins
attach to unpaired DNA strands and keep them from unpairing
topoisomerase
goes ahead of the helicase and relieves stress in the strands
primer
initial nucleotide chain producing during DNA synthesis
DNA Polymerases
Catalyze the synthesis of new DNA by adding nucleotides to a preexisting chains
primase
enzyme that synthesizes primer
Okazaki Fragments
series of segments of lagging strand, and are synthesized discontinuously, about 1000-2000
DNA Ligase
Joining the sugar-phosphate backbones of the Okazaki fragments
Mismatch repair
enzymes remove and replace incorrect nucleotides
Nuclease
The enzyme that cut outs the mismatched nucleotides of DNA
Nucleotide excision repair
A method of replacing wrong nucleotides by using DNA Polymerase and DNA Ligase
Telomerase
Catalyzes the lengthening of telomeres in eukaryotic germ cells, restoring orginal length
Nucleiod
The area where DNA is stored in prokaryotic cells, not surrounded by a membrane
Chromatin
The protein that DNA wraps its self around when it coils up
Heterochromatin
Interphase chromatin, visible by light microscope as irregular clumps
Euchromatin
Less compacted and more dispersed
Nucleic Acid Hybridization
The base pairing of one strand of a nuclei acid to a complementary sequence of a another strand
Genetic Engineering
Direct manipulation of genes for practical purposes
Plasmid
Small circular DNA molecules that replicate separately from the bacterial chromosome
Recombinant DNA
When two different DNA sources are combined in vitro
Gene cloning
Production of multiple copies of the same gene
Restriction enzymes
Cut DNA at specific locations
Restriction Site
Point where the restriction enzyme is put in use and cuts the DNA
Restriction Fragment
The result of the cutting of the DNA by restriction enzymes
Gel electrophoresis
To see restriction fragment this technique separates by lengthy
Cloning Vector
A DNA molecule that can carry DNA into a host cell and replicate there