Ch. 14 Non-Mammalian Kidneys/Other Osmoregulatory Organs Flashcards

1
Q

marine hagfish kidneys

A

nephrons have glomeruli but no tubules

Bowman’s capsules empty directly into collecting ducts

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2
Q

kidneys of freshwater teleosts whose bodies are hyperosmotic

A

produce large volumes of dilute urine

larger and more glomeruli

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3
Q

kidneys of marine teleosts whose bodies are hypoosmotic

A

have no glomeruli or bowman’s capsules

produce small volumes urine by secretion

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4
Q

elasmobranch Raja erinacea kidney

A

has countercurrent system but kidney is different

retains urea and does not make concentrated urine

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5
Q

what 3 animals have glands that secrete salt

A

elasmobranches

marine bids

some reptiles

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6
Q

are marine elasmobranches hypo or hypertonic to seawater, and what is their sodium content in comparison?

A

slightly hypotonic

sodium is much lower than seawater – continual influx of NaCl

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7
Q

elasmobranch rectal gland purpose

A

makes and excretes a concentrated salt solutions to regulate extracellular fluid volume

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8
Q

elasmobranch rectal gland structure

A

lots of blind-ended tubules surrounded by blood capillaries

tubules drain into a duct –> intestine near the rectum

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9
Q

the fluid made by the elasmobranch rectal gland has a slightly higher ______ content than seawater but is ___osmotic to blood plasma

A

salt,-iso-osmotic

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10
Q

the elasmobranch blood is slightly ____osmotic to seawater and contains

A

hyperosmotic

lots of urea and TMAO

hyposmotic amount of salt

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11
Q

do urea and TMAO appear in elasmobranch rectal gland excretions?

A

no, just NaCl

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12
Q

how does the elasmobranch rectal gland work

A

Apical - Cl- channel into seawater

Basolat - very folded with lots of Na/K pumps and Na/2Cl/K cotransport

Cl moving into seawater causes transmembrane potential that moves Na out paracellularly against gradient

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13
Q

where is the salt gland located in birds/reptiles

A

depressions in the skull above the eyes

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14
Q

structure of marine bird salt glands

A

many lobes with secretory tubules and a central canal

duct runs through the beak and into the nostrils

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15
Q

marine bird salt gland secretory epithelial

A

like typical salt secreting epithelium

lots of Na/K pump in basolat and Na/2Cl/K cotransport

Cl- channels in apical

Cl- moving out causes Na to move out paracellularly against Na gradient

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16
Q

is the salt solution in the bird salt glands hyper or hypo osmotic to blood

A

hyperosmotic

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17
Q

the birds that make the most concentrated salt solution have…

A

largest secretory cell and a long paracellular channel between cells

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18
Q

is there a countercurrent system in bird salt glands

A

yes

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19
Q

will ouabain inhibit bird salt glands

A

yes, lots of mitochondria there to make ATP to drive Na/K pumps

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20
Q

what stimulates bird salt gland production

A

raise in extracellular fluid volume and salt content

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21
Q

why can’t mammals drink seawater even though we have salt-secreting cells in the ascending limb of the loop of Henle

A

arrangement of cells doesn’t allow producing a hypertonic salt soln that can be excreted

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22
Q

in teleost fishes, what major function do gills perform

A

managing osmotic stress

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23
Q

structure of teleost gills

A

large epithelium for gas exchange/salt transport

chloride cells invaginated in folded basolat w/ lots of mito

mucous cells form boundary btw epithelium and seawater

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24
Q

teleost chloride cell salt transport method (and Ca)

A

high level of Na/K pump and Na/2Cl/K cotransporter in basolat

Cl channels in apical memb

Cl out causes Na out paracellularly

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25
Q

in marine teleosts does the transport of salt occur against an osmotic gradient?

A

yes, so no water follows

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26
Q

uptake of salt in freshwater fish gills

A

Apical - H+ pump puts H+ into seawater, Na channels bring Na in

Basolat - Na/K pump puts Na in blood and K cycles through channels

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27
Q

chloride cells in gill epithelium of freshwater fish

A

uptake Ca from water

anion transporter in apical

lots of H+ pumps

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28
Q

migrating fish from fresh to seawater adaptations

A

proton pump that brings in NaCl down-regulated

rise in body/plasma Na causes more cortisol and growth hormone levels

hormones make chloride cells and insert into basolat –> more active Na/K pump and NaCl secretion

29
Q

migrating fish from sea to freshwater

A

paracellular gaps btw chloride and accessory cells close causing less loss of NaCl

prolactin increases –> lower chloride cells and apical pits to disappear

Na/K pump activity falls

upregulate proton pump

30
Q

what invertebrates make concentrated urine

A

insects

spiders

31
Q

urine in mollusks and crustaceans

A

like humans, high [ ] = excretion (inulin, glucose) and others reabsorbed

depends on bp

32
Q

phlorizin drug that blocks glucose transport in mollusks and crustaceans

A

glucose still appears in urine

glc must enter urine as a part of filtrate

33
Q

in crayfish what is the major organ of osmoregulation

A

antennal gland with a coelomosac (like glomerulus)

34
Q

downside of a filtration-reabsorption system like kidney

A

high energy cost

needs high intake of salts

35
Q

do insects have a large surface to volume ratio?

A

yes

places a lot of osmotic demand

36
Q

can the locust regulate the ionic strength of its hemolymph?

A

yes

during dehydration the hemolymph decreases by 90% but ionic concentration is maintained

37
Q

the osmoregulatory system of locusts and insects consists of the ___________ tubules and _______

A

Malpighian tubules, hindgut (ileum colon and rectum)

38
Q

the closed ends of the long thin Malpighian tubules lie in the _________

A

hemocel – hemolymph containing body cavity

39
Q

the Malpighian tubules empty where

A

alimentary canal at the junction of the midgut and hindgut

40
Q

pathway of secretion through the Malpighian tubules

A

tubules –> hindgut where it is dehydrated –> rectum –> concentrated urine through anus

41
Q

the presence of a ________ system for respiration in insects diminishes the importance of an efficient circulatory system

A

tracheal

42
Q

do the Malpighian tubules get a direct arterial blood supply?

A

no, they are surrounded by hemolymph at equal pressure

b/c no pressure, no filtration system

43
Q

urine formation by Malpighian tubules

A

KCl and NaCl from hemocoel into lumen with waste like uric acid

most NaCl and KCl returned to hemolymph across rectal wall

ionic editing occurs in hindgut

44
Q

what ion drives the formation of urine in the Malpighian tubules

A

K+, other substances follow passively

45
Q

hindgut of locus Schistocerca

A

Apical - Cl pump into cell, K channel into cel, Na uptake + aa uptake OR ammonium excretion, H+ pump into urine

Basolat - K and Cl channels into blood, Na/K pump, Cl/bicarb antiport moves Cl into cell and bicarb into hemolymph

46
Q

mealworm beetle Tenebrio countercurrent system

A

Malpighian tubules touch hemocel

perirectal space in middle

rectal lumen and anus below perirectal space

47
Q

mealworm beetle Tenebrio mechanism

A

water drawn osmotically from rectum into Malpighian tubules by KCl gradient from active transport

highest osmolarity by anus

urine:blood osmolarity of 10:1

48
Q

feedback mechanism in blood sucking Rhodnius

A

after sucking blood, 2-3min go by and Malpighian tubules increase secretion by 1000x

artificial bloating doesn’t cause this

diuretic hormone in response to ingested blood – serotonin?

49
Q

when aa are catabolized the aa group NH2 is released (_________) or transferred to another molecules for reuse…aa not salvaged for resynthesis must be…

A

deamination

dissolved in water and excreted to avoid a toxic rise in nitrogenous waste

50
Q

most excess nitrogen is excreted as what 3 things

A

ammonia

urea

uric acid

51
Q

is ammonia more toxic than urea or uric acid

A

yes

52
Q

how does excretion of ammonia occur

A

diffusion

requires large volume of water

53
Q

urea excretion

A

requires less water than ammonia but needs ATP to synthesize

54
Q

uric acid is excreted as what

A

not soluble so white pasty precipitate of bird poop

55
Q

what determines the nature of nitrogen excretion

A

availability of water

aquatic excrete across gills

terrestrial animals excrete urea or uric acid via kidneys

56
Q

ammonotelic

A

excrete nitrogenous waste as ammonia

teleosts and aquatic invertebrates

57
Q

which form of ammonia are cell membranes permeable

A

NH3

not NH4+

58
Q

how do teleosts excrete ammonia

A

H+ and CO2 excretion cause acidification of water next to gill surface

this traps NH3 as NH4 as NH3 passively diffuses out of gills

59
Q

how is a small amount of ammonia excreted in mammalian kidneys

A

glutamine released from liver –> blood –> kidney is deaminated by kidney tubule cells and ammonia goes into the lumen

it takes up a proton –> NH4 which cannot diffuse out

60
Q

high ammonia levels in aquatic environments are toxic, especially at high __

A

pH

impairs excretion so glutamine may be stored instead

fishes also downregulate ammonia synthesis

61
Q

toxicity of ammonia

A

changes tertiary structure of proteins

disrupts ion transport b/c substitutes as K+

62
Q

squids shrimp and tunicates do what to ammonium ions

A

collect it in acidified chambers and substitute it for Ca and Mg

63
Q

urea-excreting (ureotelic) animals

A

excrete nitrogenous waste as water-soluble urea

vertebrates use ornithate-urea cycle

teleosts/invert use uricolytic pathway

64
Q

ornithate-urea cycle of vertebrates

A

2 NH2 and 1 CO2 added to ornithine to make arg

arginase removes urea to regenerate ornithine

65
Q

how do elasmobranches use ornithate-urea cycle

A

use the urea as an osmolyte to inc body osmolarity

excrete urea across gills

66
Q

uricolytic pathway

A

urea made from uric acid from transamination of asp or na metabolism

67
Q

gout

A

buildup of insoluble uric acid crystals

68
Q

uric acic-excreting animals (uricotelic)

A

birds, reptiles, arthropods

removes 4 N atoms per/uric acid

69
Q

how is uric acid excreted

A

proximal tubule of nephron via organic anion secretory mechanism

in birds competes for transport in kidney with PAH