Cellular Respiration Lab

Question 1

Equation of cellular respiration

Answer 1

C6H12O6 + 6O2 –> 6CO2 + 6H2O + energy

Question 2

3 stages of cellular respiration

Answer 2

glycolysis
kreb cycle
electron transport chain

Question 3

Where does glycolysis take place?

Answer 3

in the cells cytoplasm

Question 4

What molecule is broken down in glycolysis?

Answer 4

glucose

Question 5

What molecules are used to break down glucose and fructose?

Answer 5

ATP

Question 6

What molecules are made midway of glycolysis?

Answer 6

2 Glyceraldehyde 3-phosphate

G3P

Question 7

What high energy electron is reduced in glycolysis?

Answer 7

NAD+ is reduced (gains electrons) into NADH because that electron is picked up to generate ATP

Question 8

What molecule is phosphorylated in order to break down G3P?

Answer 8

2 ADP is phosphorylated into 2 ATP twice

Question 9

What is the end product of glycolysis?

Answer 9

2 pyruvate molecules, 4 ATP, and 2 NADH

Question 10

Before entering the Kreb Cycle, what process does pyruvate have to go through?

Answer 10

Pyruvate Oxidation

Question 11

Where does Pyruvate Oxidation occur?

Answer 11

from the cytoplasm, that pyruvate molecule does into the mitochondrial matrix

Question 12

What is done in pyruvate oxidation?

Answer 12

a CO2 is detached from pyruvate, an NAD+ is reduced into NADH+, and a coenzyme A is attached

Question 13

What is the end product of Pyruvate Oxidation?

Answer 13

Acetyl-CoA (2 carbon molecule)

Question 14

In the Kreb Cycle, what does Acetyl-CoA bind to?

Answer 14

Oxaloacetate a 4 carbon molecule

Question 15

What molecule forms once Acetyl-CoA and Oxaloacetate bind?

Answer 15

a 6 carbon molecule Citrate also known as Citric Acid

Question 16

Where does the Kreb Cycle take place?

Answer 16

mitochondrial matrix

Question 17

What is the end products of the Kreb Cycle after two rounds?

Answer 17

6 CO2, 6 NADH, 2 FADH2, and 4 ATP

Question 18

What molecule is added to form malate from fumarate in the Kreb Cycle?

Answer 18

H2O made from glycolysis is used to create malate

Question 19

Where does the Electron Transport Chain occur?

Answer 19

mitochondria

Question 20

What does the Electron Transport Chain do?

Answer 20

reduce electron carriers NADH and FADH2 to donate those electrons to produce ATP

Question 21

Through what process is ATP produced?

Answer 21

a proton gradient
chemiosmosis
hydrogen ion concentration

Question 22

How many hydrogen ions generate 1 ATP?

Answer 22

four hydrogen ions

Question 23

What molecule is final electron acceptor?

Answer 23

O2 is a “trash can” for low energy electrons so H2O can be made

Question 24

How many ATP molecules are made?

Answer 24

30 in Eukaryotes

32 in Prokaryotes

Question 25

What process of cellular respiration is anaerobic?

Answer 25

glycolysis

Question 26

Where does lactate fermentation occur and what does it do?

Answer 26

in skeletal muscle, and it is when a glucose molecule under goes glycolysis, produces pyruvate but that pyruvate is turn into lactase due to the oxidation of 2 NADH molecules into 2 NAD+

Question 27

What are inhibitors of cellular respiration?

Answer 27

Flouride inhibits enzymes that requires Mg as a cofactor

Question 28

What is a cofactor of cellular respiration?

Answer 28

Magnesium is a cofactor which is a nonprotein component required by an enzyme function