Cell Biology- Microscopy Flashcards
How do you calculate magnification?
Magnification = image size ÷ actual size
How do you calculate image size?
Image size = magnification x real size
How do you calculate real size?
Real size = image size ÷ magnification
What are the units for micrometers and nanometers?
μm = Micrometre
nm = nanometre
How do you convert between mm and μm?
1mm = 1000μm
mm —> μm = x 1000
μm —> mm = ÷ 1000
______ microscopes were developed first.
Light microscopes were developed first.
_______ microscopes give greater magnification and resolution.
Electron microscopes give greater magnification and resolution.
what is 1cm in standard form
1 x 10^-2m
what is 1mm in standard form
1 x 10^-3m
1 um(mico metre) in standard form
1 x 10^-6m
what is 1nm in standard form
1 x 10^-9m
what does 1 order of magnitude mean
10 x bigger
explain the rule when talking about order of magnitude
every order of magnitude is 10x greater then the one before
what can you do to help work out the order of magnitude
count the zeros
10x = 1 order
100x = 2 orders
1000x = 3 orders
parts of a microscope
- center of microscope is called the stage ( where the slide gets clipped in)
- below is a lamp, light from this passes through to the slide
- above the stage is the objective lenses usually 3 magnifications
- eyepiece is where we look through
required practical
- place slide onto stage - clip to hold in place
- select the lowest power objective lense
- slowly turn the coarse focusing dial to make the lense almost touch the slide
- look down through the eyepiece slowly turn the coarse focusing dial to increase the distance between the lense and slide
- use the fine focusing dial to bring the cells into a clear focus
- use a pencil to draw some of the cells and structures you can see - include magnification scale
when using the magnification equation what unit is used
mm
