Cell Biology- Microscopy

Question 1

How do you calculate magnification?

Answer 1

Magnification = image size ÷ actual size

Question 2

How do you calculate image size?

Answer 2

Image size = magnification x real size

Question 3

How do you calculate real size?

Answer 3

Real size = image size ÷ magnification

Question 4

What are the units for micrometers and nanometers?

Answer 4

μm = Micrometre
nm = nanometre

Question 5

How do you convert between mm and μm?

Answer 5

1mm = 1000μm

mm —> μm = x 1000
μm —> mm = ÷ 1000

Question 6

______ microscopes were developed first.

Answer 6

Light microscopes were developed first.

Question 7

_______ microscopes give greater magnification and resolution.

Answer 7

Electron microscopes give greater magnification and resolution.

Question 8

what is 1cm in standard form

Answer 8

1 x 10^-2m

Question 9

what is 1mm in standard form

Answer 9

1 x 10^-3m

Question 10

1 um(mico metre) in standard form

Answer 10

1 x 10^-6m

Question 11

what is 1nm in standard form

Answer 11

1 x 10^-9m

Question 12

what does 1 order of magnitude mean

Answer 12

10 x bigger

Question 13

explain the rule when talking about order of magnitude

Answer 13

every order of magnitude is 10x greater then the one before

Question 14

what can you do to help work out the order of magnitude

Answer 14

count the zeros
10x = 1 order
100x = 2 orders
1000x = 3 orders

Question 15

parts of a microscope

Answer 15

• center of microscope is called the stage ( where the slide gets clipped in)
• below is a lamp, light from this passes through to the slide
• above the stage is the objective lenses usually 3 magnifications
• eyepiece is where we look through

Question 16

required practical

Answer 16

1. place slide onto stage - clip to hold in place
2. select the lowest power objective lense
3. slowly turn the coarse focusing dial to make the lense almost touch the slide
4. look down through the eyepiece slowly turn the coarse focusing dial to increase the distance between the lense and slide
5. use the fine focusing dial to bring the cells into a clear focus
6. use a pencil to draw some of the cells and structures you can see - include magnification scale

Question 17

when using the magnification equation what unit is used

Answer 17

mm