CB4 Genetic Engineering (Higher Only) Flashcards
1
Q
What 2 enzymes are used in genetic engineering?
A
Restriction, ligase
2
Q
What 2 vectors can be used in genetic engineering?
A
Bacterial plasmid or virus
3
Q
What 2 steps are restriction enzymes used for in genetic engineering?
A
Cutting the desired gene out of the genome, cutting open the bacterial plasmid
4
Q
What is a DNA “sticky end”?
A
A staggered cut of DNA, leaving exposed, unpaired bases.
5
Q
What step is ligase enzyme used for in genetic engineering?
A
Attaching the desired gene to the bacterial plasmid
