CB1 Microscopes Flashcards
What are the names of the 2 types of lenses on a microscope?
Eyepiece and objective
Where are slides placed on the microscope?
Stage
What are used to focus the image?
Focussing wheels
How do we calculate the total magnification of an image?
Eyepiece lens magnification x objective lens magnification
What does magnification mean?
How many times larger the image of an object appears.
What does resolution mean?
The smallest distance that can be measured. Affects how clear the image is.
Why do we use stains when preparing a slide?
Makes cells and parts of cells more visible.
Why is it important the specimen on the slide is thin and flat?
So light can pass through it.
What are the 2 types of microscope?
Light, electron
Which type(s) of microscope allow us to observe large organelles such as the nucleus, mitochondria and chloroplasts?
Light, electron
Which type(s) of microscope allow us to observe small organelles such as the cell membrane and ribosomes?
Electron
What are the 3 main differences between light and electron microscopes?
Use different radiation (light / beam of electrons), electron has higher resolution, electron has higher magnification
Put the following prefixes for units in order from largest to smallest and include the symbols: micro, pico, milli, nano
Milli (m), micro (µ), nano (n), pico (p)
What is the formula to calculate the magnification of an image?
M = I /A
Describe the 3 steps for assembling a microscope slide of plant cells
Place a thin piece of the plant on a microscope slide using tweezers, add a drop of stain, gently place a coverslip over the sample
