Cardiology - Lipids Flashcards
What is the role of apolipoproteins?
Activate enzymes needed for lipoprotein metabolism
What is the function of apoprotein component of lipoproteins?
Structural support
Receptor recognition
Enzymatic activity
Where is ApoB-100 made?
In the liver
Where is ApoB-48 made?
In the intestine
What is Apo-AI?
The major protein in HDL that has been inversely correlated with arteriographic evidence of coronary disease
it activates the enzyme lecithin-cholesterol acyltransferase (LCAT)
What does LCAT (lecithin-cholesterol acyltransferase) do?
allows the HDL particle to convert cholesterol obtained from peripheral tissues to cholesterol ester
What is Apo-B 100
The primary apoprotein of LDL which allows recognition of the particle by the LDL receptors on cell surfaces
What are the genes that synthesise Apo-B48 and Apo-B 100
Trick question. They are made by the same gene.
Where is Apo-E found?
In VLDL particles AND in chylomicrons, IDLs and HDLs.
What does ApoE do?
ApoE helps remove chylomicrons from the serum to the liver
What is the characteristic pathology of Type III hyperlipoproteinaemia?
Most Type III hyperlipoproteinaemia patients are homozygous to apoprotein E2/2 genotype
Characterised by premature atherosclerosis
(BOTH hyperchol and hypertriglyceridaemia)
SECONDARY causes of VLDL overproduction
Excessive VLDL secretion by liver is seen in:
- high carb diets
- alcohol
- obesity
- nephrotic syndrome
- cushing’s syndrome
What are the two PRIMARY causes of excessive VLDL production by the liver?
Familial combined hyperlipidaemia (FCHL)
Lipodystrophy
What are the features of Familial Combined Hyperlipidaemia (FCHL)?
- elevated TG and LDL-C
- low HDL
- Cause of hyperlipidaemia in 20% of those with IHD <60yrs old
What are the features of lipodystrophy?
- Truncal adiposity with decreased fat in buttocks and extremities
- insulin resistance and T2DM
- hepatosteatosis
- elevated TG and LDL-C
What are the PRIMARY causes of impaired lipolysis of TG-rich lipoprotein (with VERY HIGH triglycerides)?
Familial chylomicronaemia syndrome Familial hypertriglyceridaemia (FHTG)
Features of Familial chylomicronaemia syndrome
PROFOUNDLY elevated chylomicrons
Fasting TG VERY high
Recurrent pancreatitis
Lipaemia retinalis
Xanthomas
Hepatosplenomegaly
** NOT associated with coronary artery disease **
What is the inheritance of the two types of familial chylomicronaemia syndrome?
LPL deficiency: Autosomal Recessive
Apo-C II Deficiency: Autosomal Recessive
What are the features of familial hypertriglyceridaemia (FHTG)?
ELEVATED fasting TG
Average/low LDL-C
Low HDL-C
NOT ELEVATED APO-B
NOT ASSOC. WITH CAD
What are the dyslipidaemias caused by impaired hepatic uptake of ApoB-containging lipoproteins?
Type 3 Hypercholesterolaemia
Sitosterolemia
Cholesterol Ester Storage Disease
What is Type 3 Hypercholesterolaemia?
aka Autosomal Dominant Hypercholesterolaemia due to Mutations in PCSK9
dyslipidaemia caused by impaired hepatic uptake of ApoB-containging lipoproteins
- autosomal dominant
- rare
- responds well to PCSK9 inhibition
What mutation causes Type 3 Hypercholesterolaemia?
Mutation in PCSK9
What is Sitosterolaemia?
rare, autosomal recessive
dyslipidaemia caused by impaired hepatic uptake of ApoB-containging lipoproteins
Mutations in ATP-Binding Cassette (ABCG5 and ABCG8)
ALSO get anisocytosis, poikilocytosis and splenomegaly
–> respond VERY WELL to dietary modification and ezetimibe
What mutation causes Sitosterolaemia?
Mutations in ATP-Binding Cassette (ABCG5 and ABCG8)