Building A Polypeptide

Question 1

In Eukaryotes, in what order does the small sub unit bind to the mRNA and to the specific initiator tRNA

Answer 1

Small subunit is already bound to the initiator tRNA

Question 2

Where along the mRNA does the subunit bind to in 1) Bacteria 2) Eukaryotes

Answer 2

1) at a specific RNA sequence, upstream of the start AUG Codon 2) to the 5’ cap of the mRNA and mRNA moves along till initiator tRNA detects AUG

Question 3

What is the translation initiation complex

Answer 3

mRNA, Initiator tRNA, Small ribosome unit, large ribosome unit

Question 4

What is the energy for the initiation factors to bring all the components together

Answer 4

Hydrolysis of GTP

Question 5

Hydrolysis of GTP increases what

Answer 5

Accuracy and efficiency of codon-anticodon recognition

Question 6

Describe the peptide bond being catalysed by the large subunit

Answer 6

Carboxyl end of the previous amino acid on the polypeptide chain and the amino group on the new amino acid

Question 7

Polypeptide is always synthesised in what direction

Answer 7

From initial MET amino group N terminus end to the final amino acid at carboxyl C Terminus end

Question 8

When a stop codon reaches site A what happens

Answer 8

A protein shaped like aminoacyl tRNA (release factor) is binded to the stop codon in site A

Question 9

Binding of the release factor does what

Answer 9

Hydrolisation of the bond between the completed polypeptide and the tRNA in site P

Question 10

300 Nucelotides equals how many amino acids

Answer 10

100

Question 11

Redundancy no…

Answer 11

Ambiguity

Question 12

3 stop codons

Answer 12

UAA UAG UGA

Question 13

In bacteria, in what order does the small sub unit bind to the mRNA and to the specific initiator tRNA

Answer 13

Any order