Postrep Mismatch repair Flashcards
1
Q
Mismatch repair must be able to distinguish between what two strands
A
parental and newly synthesised strand that contains the mismatched base
2
Q
In the newly replicated DNA, what happens to the parental stand which allows the mismatch repair system to distinguish the new strand from the old strand
A
Parental strand is methylated
3
Q
Mismatch repair requires the products of what 4 genes
A
mutH
mutL
mutS
mutU
4
Q
what does mutS do
A
recognises mismatches and binds to them to initiate repair process
5
Q
mutH and mutL do what
A
join the complex
6
Q
mutH cleaves what and where
A
the unmethylated strand at hemimethylated GATC sequences on either side of the mismatch
7
Q
mutU is also known as what
A
Helicase II
8
Q
The A in GATC sequences is methylated when
A
after DNA replication
