Batceriophage Vectors Flashcards
why can bacteriophage vectors be used as vectrs
central one third of genome is not essential for lytic growth and so can be replaced by foreign DNA
what is a concatmer
multiple genomes link by their cos sites forming a linear structure
why are cosmid vectors used
to clone larger pieces of DNA (more than 15kb)
cosmids are hybrids between what
plasmids and lambda (cos site) Phage
Lambda phage is how many stranded and form what
Double stranded
terminal ends are complementary and form cos sites
how is the lambda genome replicated
Rolling circle replication
Shuttle vectors are what?
Usually a plasmid and it can be propagated in 2 different host species
example of shuttle vectors
Yeast gene example
Yeast Artificial Chromosomes (YACs) contain what
Origin of replication Yeast centromere 2 yeast telomeres a selectable marker polycloning site
Bacterial artificial chromosomes (BACs) and Bacteriophage P1 artificial chromosomes (PACs)
1) accept what size insert
2) less or more complex than YACs
3) have now replaced
1) 150-300kb
2) Less complex
3) Replaced YAC vectors
What is a genomic DNA library
Set of DNA clones that collectively contain the entire genome of any given organism
What is a chromosome specific library
Set of DNA clones that collectively contains all the DNA from a given chromosome
A complementary DNA (cDNA) library contains
a DNA copy of all the mRNA expressed from an organism
the number independent clones required to produce a library that has a certain probability (P) of containing a given DNA sequence is given by the formula:
N= ln (1 - P)/ln (1 - f)
look at notes on how to make cDNA
okay
