BP/Kaplan/AAMC B/B Flashcards
michaelis menten
mm- vmax (s)/ km+(s)
orthosteric
only at the active site
how do phosphatases remove a phosphate
phosphatases do use water in their removal of a phosphate
how does telomerase replicate dna? bc of this, what could u call telomerase?
Telomerase uses an RNA template to replicate DNA at chromosome ends (telomeres). As telomerase reads an RNA template, it is RNA-dependent DNA polymerase.
non coding transposable elements- what can they do, and what is the effect of this?
Non-coding transposable elements can insert themselves into gene sequences, which is thought to thereby increase genetic diversity and able to accelerate evolution
what is differential repro
Differential reproduction is the concept that some phenotypes are more fit than other phenotypes. Individuals with such phenotypes will survive and reproduce more frequently. This is the basis of natural selection, which drives evolution.
species def
A species is normally defined as a genetically-similar group of organisms that can breed and produce fertile offspring. A species is reproductively isolated from other groups and cannot produce viable offspring with other species groups.
what does HW apply to? what is it used for? is it real or hypothetical and why
Hardy-Weinberg equilibrium only applies to populations that are not evolving. It’s used to determine whether or not a population is evolving by comparing it to the predictions of Hardy-Weinberg equilibrium and to calculate genotype and allele frequencies within a population. Hardy-Weinburg is hypothetical, as all populations experience some form of evolution.
whats inclusive fitness
Inclusive fitness expands the idea of genetic success beyond the individual to one’s relations. Since all of the wolves within a pack are closely related, they share many of the same genes. One wolf might help another wolf because their mutual survival promotes the survival of their shared alleles.
explain p and q in HW eqn
p and q refer to ALLELE FREQUENCY. p^2 and q^2 refer to PHENOTYPE FREQUENCY
gene flow
Gene flow is the movement of alleles between populations due to the migration of individuals between those populations.
bottleneck effect
The bottleneck effect occurs when population allele frequencies change in response to a random decrease in population size due to external events, such as natural disasters. not related to adaptations that may already be preexisting.
what can genetic drift cause and what can happena s a reult
Genetic drift can cause allele fixation, which occurs when one or more alleles of a gene are randomly eliminated from a population over time, resulting in only a single possible genotype at that locus.
what is fitness
Fitness is a quantitative measure of natural selection, so it cannot be said to result in natural selection.
when are viruses considered positive or negative sense
Positive-sense single-stranded RNA viruses contain RNA that can function as mRNA and be directly translated into protein. Viruses are considered negative-sense when their RNA is complementary to mRNA and must be copied into mRNA by the viral enzyme RNA replicase prior to translation.
what is a retrovirus
Retroviruses are single strand RNA viruses. As such, their genetic material must first be reverse transcribed into DNA before it can integrate into the host genome.
are retroviruses + or - sense? explain what happens to their genome and how they share their genetic info. can they survive on their own without other living things
Retroviruses are positive-sense ssRNA viruses. These pathogens have a single-stranded RNA genome that must be reverse transcribed to form DNA. The DNA is then transcribed into RNA and used to synthesize viral proteins. It should be noted that viruses are not living organisms, as they cannot survive on their own without other living organisms.
what is the process for interpreting a figure. what is the order in which things should be read and looked at?
The process of interpreting a figure involves going from the general to the specific, and then back to the general. In other words, one should first read the figure legend to determine what the figure is about in general terms. Then, identifying the independent and dependent variables is a necessary prerequisite for understanding the relationships portrayed in the figure, which are further underscored by the units. Based on an analysis of the details of the figure, one can zoom out to make the figure tell a story.
what is penetrance and expressivityt
Penetrance expresses a binary concept that a genotype’s corresponding phenotype will or will not be expressed. Expressivity expresses the degree to which a genotype’s phenotype s expressed, leading to a variable resultant phenotype.
what are wild type traits
The term “wild type” refers to the traits an organism typically exhibits when found in nature. These may be dominant traits but not always.
do linked genes assort independently and why or why not
Linked genes have a high likelihood of NOT assorting independently. If offspring phenotypes differ significantly from the expected Mendelian ratio of 9:3:3:1, the genes may well be linked.
Concentration gradients established by primary active transport do what
This describes a concentration gradient. Concentration gradients established by primary active transport drive secondary active transport.
what is primary active transport defined by? what does it not depend on? can both secondary and primary AT move molecules against conc gradient
Primary active transport is defined by energy consumption to move molecules against their concentration gradient. Primary active transport does not depend on whether or not those molecules can diffuse back across the membrane. Both secondary and primary active transport can move molecules against their concentration gradient.
what do early endosomes do and not do
Early endosomes are responsible for sorting endocytosed vesicles and identifying what to do with the contents. They are not responsible for the actual degradation themselves.
does fac diff need energy and does passive dif? what do both rely on and what does this mean? explain how they are connected
Facilitated diffusion does not require any energy (either directly or indirectly) and nor does passive diffusion. Both rely on diffusion, which is the tendency of molecules to spread out and their concentrations throughout a solution to equilibrate. Facilitated diffusion is passive diffusion through a protein channel, and it helps very polar molecules cross the hydrophobic plasma membrane core.
what is a reporter gene
A reporter gene encodes a protein that creates visible phenotypic change. Whether the phenotypic change occurs allows the scientist to determine whether or not the recombination was successful, as successful recombination will include in the expression of the reporter gene.
conjugation
Conjugation refers to the transfer of genetic material directly between bacteria via a sex pilus (a bridge-like passage between two bacteria).
what doesEngineering plasmids with antibiotic resistance genes enable? When we treat a plate of bacteria with an antibiotic, what happens
Engineering plasmids with antibiotic resistance genes enable them to be separated on the basis of antibiotic resistance. When we treat a plate of bacteria with an antibiotic, only those containing the antibiotic resistance gene will survive. Those that contain this antibiotic resistance gene will also contain the gene of interest.
Glycoglycerolipids always have… and what about sphingolipids
Glycoglycerolipids always have a carbohydrate group attached, but only some sphingolipids have an attached carbohydrate group.
Sphingomyelin- what is it and what does it cluster with and why
Sphingomyelin is a glycolipid with a sphingosine backbone in place of the glycerol that is found in phospholipids and glycoglycerolipids. Sphingomyelin clusters with cholesterol to make lipid rafts, which modulate membrane fluidity and serve as organized clusters for various signaling processes.
what are glyceroglyoclipids and how are they similar to plipids and how are they diff
Glyceroglycolipids are a glycolipid type, which is a major class of membrane lipids. Glyceroglycolipids are similar to phospholipids in that they have a glycerol backbone and two fatty acid tails. However, glyceroglycolipids replace their phosphate group with a carbohydrate group.
esterification. also what occurs with esterification in plipids
Fatty acids can be attached to a glycerol molecule through a process known as esterification. In phospholipids, this happens to two out of the three hydroxyl groups. The remaining hydroxyl group is phosphorylated.
glycoglycerolipids v spingolipids- compare backbones, and head groups
Glycoglycerolipids and sphingolipids differ from each other in their backbone molecule: Glycoglycerolipids have a glycerol backbone and sphingolipids have a sphingosine backbone. Glycoglycerolipids and sphingolipids can contain the same hydrocarbon head group, but they still belong to their respective glycolipid subclasses because of their backbone.
hybridization
Hybridization is the process where single-stranded DNA or RNA form base pairs with its complementary strand. This is used in PCR to anneal DNA strands to each other.
what type of molecule moves further in gel ephoresis
Negatively charged molecules, not positively charged ones, tend to move further during gel electrophoresis.
PCR is used to
PCR is used to amplify genetic material prior to ligating genetic material into vectors. Thus, this is a potential application of PCR.
n Sanger sequencing…
In Sanger sequencing, all four ddNTPs are added to a single tube where DNA replication occurs.dNTPs elongate the DNA chain, while ddNTPs terminate replication.
what happens in primer extnesion
During primer extension, chains of various lengths are produced, all complementary to the template DNA. Since new strands are synthesized from 5’ to 3’, the 3’ end of the complementary DNA chain will be nearest to the well, while the 5’ end will be farthest. \
why is glucose not likely to cross the plasma membrane
Glucose’s polar groups make it less likely to be able to cross through the plasma membrane.
p53- activator or repressor
p53 is a transcription activator, not a repressor. It activates the transcription of genes that inhibit cell cycle progression.
defs of cytokines and microglia
Cytokines are protein-based signaling molecules secreted by cells of the immune system. Microglia are a macrophage derivative, a type of cell from the immune system, and as such will most likely secrete cytokines to modulate immune responses.
when can second msngers be made/ destroyed? can they be localized to other areas of the cell? give an example
Second messengers can be created or destroyed at times when the cell needs to turn the signal on or off, respectively. Second messengers can also be localized to specific areas of the cell in which they are needed, such as the cytoplasm or the endoplasmic reticulum.
Allergies occur when
Allergies occur when the immune system attacks a foreign molecule, such as pollen, that is actually harmless. In this example, the process begins when pollen in the air enters the lungs and the dendritic cell recognizes pollen as an antigen. The dendritic cell engulfs and presents pollen fragments on its MHC class II receptor to T cells. B cells differentiate into plasma cells which produce antibodies against the pollen.
do the intercostal muscles have an active role in inhalation? when may they contract? is exhalation passive or active
The intercostal muscles do not have an active role in inhalation. Intercostal muscles may contract during exhalation to more forcefully expel air but they are not necessary for exhalation either. Exhalation can be an active or passive process.
why is breathing active
Breathing is an active process because ATP is required for the diaphragm to contract and initiate inhalation
how does oxygen get to the bstream
Oxygen is transported out of the alveoli and into the bloodstream via passive diffusion down its concentration gradient. No energy is required for oxygen to diffuse out of the alveoli and into the bloodstream to be consumed by cells.
F-actin, which stands for “filamentous actin”, is a polymer composed of
This answer choice confuses the roles of G-actin and F-actin. F-actin, which stands for “filamentous actin”, is a polymer composed of G-actin monomers.
In the trp operon, tryptophan binds to the
repressor.
With transcription factors bound to the enhancer, DNA forms a
With transcription factors bound to the enhancer, DNA forms a 3-dimensional loop that brings the enhancer sequence within close proximity of a gene’s promoter. The enhancer binds to the activator, which complexes with other transcription factors to bring RNA polymerase to the promoter.
what are enhancers and where are they found
Enhancers, DNA regions where transcription factors bind to upregulate gene expression, are found in both eukaryotic and prokaryotic DNA.
MicroRNA is responsible for and what does it do
MicroRNA is responsible for post-transcriptional silencing. These short, non-coding RNA molecules bind to mRNA, signaling its destruction or promoting degradation of its protective post-transcriptional modifications.
Silencers and enhancers are…
Silencers and enhancers are eukaryotic regulatory sequences that are bound by repressors and activators, respectively, to regulate the expression of target genes. RNA polymerase initially binds the promoter sequence before transcribing the target gene.
how does rna pol travel and synth
RNA polymerase travels in a 3’ to 5’ direction, synthesizing nucleotides in a 5’ to 3’ direction (due to the antiparallel orientation of the strands).
are only introns taken out
no, exons can b removed during alternative splicing too
which is the sense which is the antisense
RNA strands include the sense (coding) strand and the antisense (template) strand. mRNA is transcribed directly from the antisense strand, generating the same base sequence as the sense strand.
It makes SENSE to code
If a virus produces antisense RNA complementary to a gene’s promoter sequence,
the viral RNA will hybridize that sequence and prevent transcription from occurring.
Ribosome docking is fulfilled by
the 5’ cap, not the poly(A) tail.
Prokaryotic mRNA is often polycistronic, meaning
which means that a single mRNA sequence may encode multiple proteins. Eukaryotic mRNA, on the other hand, is monocistronic.
describe rna in prokaryotes- is it ready to go? can transcription and translation happen at the same time on the same rna molecule?
In prokaryotes, newly synthesized RNA is ready to go and is immediately translated into protein. Since prokaryotes do not have nuclei to separate transcription and translation, the two processes may occur simultaneously on the same RNA molecule.
how do fatty acids enter enterocytes? does this process need energy
Because fatty acids are hydrophobic, they enter enterocytes by passively diffusing through the cell membrane. This transport process is much simpler than secondary active transport and does not require energy.
do absorbed and broken down mean the same thing
NO
rh factor
The Rh factor is a type of protein on the surface of red blood cells. + means u have it and - means u dont
type a=
type a antigens
in systole, is atria pressure higher than ventricles
During systole, the pressure in the atria is not higher than the ventricles.
where does gas nutrient and waste exchange occur? what does plasma do? how is interstitial fluid in the tissues returned to the circ system?
Gas, nutrient, and waste exchange occurs in capillary beds, and plasma from the circulatory system contributes to interstitial fluid in the tissues, which is returned to the circulatory system via lymphatic vessels.
When is glycolysis unregulated? What does a high atp to amp ratio mean
Glycolysis is upregulated when the cell needs more ATP. A high ATP to AMP ratio means that the cell has a lot of ATP compared to AMP, which is an energy-depleted form of ATP. In this situation, glycolysis would be downregulated.
which are the two reactions of glycolysis that require atp. are these reversibly and what do they require in gluconeogenesis in order to proceed
This statement is true. There are two reactions in glycolysis that require ATP: The conversion of glucose to glucose 6-phosphate and the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate. Both of these steps are irreversible and require bypass reactions in gluconeogenesis.
does atp synthase pump protons
atp synthase doesnt pump protons
which are the highly regulated steps of the krebs cycle
acoa–> citrate, aketo—>succ coa, isocit—> aketo
A high ADP to ATP ratio means
A high ADP to ATP ratio means that the cell has more ADP than ATP, which suggests that the cell needs to make more energy.
This reaction is the third and final heavily regulated step of the citric acid cycle. It is inhibited by …
This reaction is the third and final heavily regulated step of the citric acid cycle. It is inhibited by high levels of NADH and succinyl CoA.
Ubiquinone can accept two electrons from…
Ubiquinone can accept two electrons from either complex I or complex II, depending on whether they came from NADH or FADH2.
describe the lipid layers of mito.
This statement claims that mitochondria have four separate lipid bilayers, but in reality, each mitochondrion has only two: the inner and the outer membrane. These two bilayers are separated by the intermembrane space.
is complex 2 embedded in the membrane? can it pump protons from the matrix to the IMS why or why not
Although complexes I, III, and IV are embedded across the inner mitochondrial membrane, complex II is a peripheral membrane protein that does not span the inner membrane. As a result, it cannot pump protons from the matrix into the IMS like the other complexes do.
globular v fibrous proteins- are they hydrophilic/phobic, what do they tend to do?
Globular proteins tend to be hydrophilic, water-soluble, and often perform enzymatic functions. Fibrous proteins serve a structural role and are usually hydrophobic.
Aminopeptidases cleave amino acids from the …
Aminopeptidases cleave amino acids from the N-terminus of a protein.
how does urea affect h bonding.
Proteins that are denatured in the presence of high urea concentrations are typically able to…
Urea disrupts hydrogen bonding, leaving the primary structure intact. Proteins that are denatured in the presence of high urea concentrations are typically able to re-fold once those concentrations are lowered.
what do reducing agents do to disulfied bridges
Reducing agents reduce disulfide bridges, breaking them back into separate cysteine residues.
what do detergents do
Detergents, which are amphipathic molecules, disrupt hydrophobic interactions in a protein.
why would gln be at the surface of a globular protein
Glutamine is a polar, uncharged amino acid, making it hydrophilic. Thus, it would be found at the surface of a properly folded, globular protein.
does an unfolded protein have more entropy than a folded protein? what about in the solvent?
An unfolded protein, which is flexible, disordered, and free to move, has greater entropy than a folded protein. In the solvent, though, an unfolded protein increases order because an organized solvation layer forms around nonpolar, hydrophobic groups in the protein that aren’t shielded in the inside of the protein when it is unfolded.
pH changes denature proteins by …
pH changes denature proteins by altering the charges on specific groups within a protein. These changes disrupt ionic interactions between the side chains of charged amino acids.
When do solvation layers form
This statement is true. Solvation layers form around all solutes in solution.
how do proteins fold with hpobic and philic groups? how do solvent molecules interact with proteins?
A protein will fold so that hydrophobic residues are oriented inward and hydrophilic residues are oriented outward. This minimizes the solvation layer. Solvent molecules interact with the polar side chains of hydrophilic residues in a less ordered fashion, which is entropically favorable.
axial skeleton is what
axial skeleton houses cns. it also includes the ribcage, so it houses those organs as well
what are synovial joins? what do they have and what does this allow for
Synovial joints are freely movable joints. Synovial joints contain a synovial cavity, which is a fibrous, lubricated capsule that allows bones to enjoy significant freedom of movement without being directly joined together or bumping into each other.
what are osteons and what do they have in them
Osteons are cylindrical structures composed of concentric rings, or lamellae, of bone matrix. In the center of the osteon is a channel known as the haversian canal, through which the bone’s blood and nerve supply passes.
what hormone is raised when blood calc is low
When blood calcium is low, parathyroid hormone (PTH) becomes elevated to increase blood calcium levels via bone resorption, decrease urinary calcium excretion, and increase calcitriol synthesis. Thus, we would expect PTH levels to be elevated.
what is the bulk of a muscle made of
Although a muscle contains connective tissue which contains collagen, the bulk of a muscle is composed of muscle tissue.
is collagen found in skin
Collagen is found in the dermis of the skin.
sds- what does it do, what does it act like, which is the anode and which is the cathode
sds makes all proteins negatively charged. ephoresis needs an external power source, indicating it acts like an electrolytic cell. this means the anode is + with a higher pot and vv for cathode. thus the proteins will go to the + anode. RED CAT and AN OX
can dopamine cross the plasma membrane
dopamine relies on vesicular transport to cross the membrane. it isnt a nonpolar sterol or small gas molecule- these can cross unassisted
shadowing
a task in which a participant repeats aloud a message word for word at the same time that the message is being presented, often while other stimuli are presented in the background. It is mainly used in studies of attention
what do late endosomes do
Late endosomes are responsible for the identification and transport of substances that have been ingested via endocytosis and need to be degraded by lysosomes. However, late endosomes are not responsible for the actual degradation themselves.
True or false: The parasympathetic nervous system has a direct inhibitory effect on the nerves of the sympathetic system.
False. The two systems tend to have opposing effects on their target tissues. However, they do not function by exerting direct effects on each other. Rather, the two systems operate in parallel, and the dominant system is decided by external stimuli.
True or false: Sympathetic ganglia generally reside close to their effector tissues.
false. Sympathetic postganglionic neurons tend to be particularly long because the target tissue resides far from the ganglion. In contrast, parasympathetic ganglia tend to reside close to the target tissue; consequently, the parasympathetic system has longer preganglionic neurons.
Autonomic ganglia are
Autonomic ganglia are collections of cell bodies in the peripheral nervous system where preganglionic neurons synapse with postganglionic neurons.
which are longer, pre or post gang
Parasympathetic preganglionic axons tend to be relatively long, while postganglionic axons are relatively short.
medial geniculate
The medial geniculate body is involved in processing the auditory, not visual pathway.
lateral geniculate
The lateral geniculate body is a key relay point for the visual pathway that ultimately terminates in the primary visual cortex in the occipital lobe.
superior colliculus
The superior colliculus is a key relay point for the visual pathway that ultimately terminates in the primary visual cortex in the occipital lobe.
inferior colliculus
The inferior colliculus is involved in processing the auditory, not visual pathway.
Select all of the following that are directly involved in the propagation of voluntary motor control.
precentral gyrus, cerebellum, basal ganglia
which isnt in the cerebrum
thalamus is not in the cerebrum
where is the rhomboencephalon
hindbrain- think rhombo= rear
which senses go thru the thalamus
audiroty, visual, gustatory