Blood Typing and Hemoglobin Determination Flashcards
0
Q
Most common method of Hgb measurement
A
Cyanohemoglobin method
1
Q
Significance of HCl in acid hematin method
A
HCl combines with hemoglobin to form acid hematin, which is compared against a standard to determine Hgb content
2
Q
Reagents in cyanohemoglobin method
A
Potassium ferricyanide
KCN
3
Q
Mechanism of cyanohemoglobin method
A
Potassium ferricyanide oxidizes Hb to Hi (methemoglobin)
KCN provides CN- to form HiCN that can be read spectrophotometrically at 540nm
4
Q
Apparatus used to determine Hb in blood bank and blood donation processes
A
Hemocue
5
Q
Spectrometry vs Nephelometry
A
Spectrometry: measures light transmitted
Nephelometry: measures scattered light
