Biology Lab 3 Results

Question 1

which objective lens is the longest

Answer 1

100x

Question 2

which is the shortest lens

Answer 2

4x

Question 2

which lens should you always start with

Answer 2

4x

Question 2

which objective lens has the smallest glass

Answer 2

100x

Question 3

as the lens gets smaller, what happens to the magnitude

Answer 3

magnitude gets higher

Question 4

the smaller the glass piece…

Answer 4

the less amount of light that will pass

Question 5

iris

Answer 5

controls how much light goes through

Question 6

which magnitude allows most light to pass through if everything stays the same

Answer 6

4X

Question 7

which one is oil immersice

Answer 7

100x; oil touches both the lens and slides

Question 8

why is oil used on the 100x

Answer 8

oil has similar properties of glass so you can see the sample at 100x

Question 9

how do you calculate total magnification

Answer 9

number oculus lens (10x) X lens using

Question 10

4x= mm

Answer 10

5mm

Question 11

10x= mm

Answer 11

2mm

Question 12

40x= mm

Answer 12

1/2mm

Question 13

how does the size of field change with different objective lens

Answer 13

the larger the magnitude, the lest diameter you can see; magnitude increases, field of view decreases

Question 14

what was the difference in viewing the threads at 4x and 10x

Answer 14

4x- can see all 3 fibers (can’t tell their on top on one another)
10x-can only see 1 fiber

Question 15

what determines the limit of a microscope

Answer 15

wavelenth

Question 16

what is the resolution limit

Answer 16

250 wavelength or 0.25micrometers; anything smaller you can’t see through light microscope

Question 17

can you see a virus with a light microscope

Answer 17

100 micrometers- cannot see with light microscope

Question 18

can you see a bacteria/prokaryotic cell with a light microscope

Answer 18

5 micrometers- can see with light microscope

Question 19

can you see a eukaryotic cell with a light microscope

Answer 19

50 micrometers- can see with light microscope

Question 20

what are the pros to using electron microscopes

Answer 20

see small objects

Question 21

what are the scientific cons to using electron microscopes

Answer 21

• can’t see live cells (electrons would kill cells)
• can’t see colors

Question 22

transmission electron microscope

Answer 22

• 2D
• see internal structure of cell
• have to split cell in half

Question 23

scanning electron microscope

Answer 23

• 3D
• see surface of cell

Question 24

is the image in the ocular inverted relative to the specimen on the stage

Answer 24

yes

Question 25

1m= mm

Answer 25

1000mm

Question 26

1mm= micrometer

Answer 26

1000 micrometer

Question 27

1 micrometer= nm

Answer 27

1000 nanometer

Question 28

look at organisms, cells, or tissues that are currently alive

Answer 28

light microscope

Question 29

look at the surface of a living cell

Answer 29

light microscope

Question 30

look at whole cells and how they connect in 3D

Answer 30

light microscope; whole cells at same time

Question 31

look at surface of a sample a high resolution

Answer 31

scanning electron microscope

Question 32

look at cross section of a sample at high resolution

Answer 32

transmission electron microscope; had to cut cell in half