Biology Lab 2 Results Flashcards
what makes lipids special
they don’t have monomers and are nonpolar
what is the purpose of using HCL
the concentrated HCl, when heated, hydrolyzes the polysaccharides; HCl is a catalyst
what were the results of the carb section of lab
Unknown A was glucose
Unknown B was sucrose
Unknown C was starch (starch test tubes turned blue when mixed with benedicts reagent)
tube 3- HCl should not do anything
tube 5- blue color=sucrose
tube 6- should change color, sucrose hydrolyze with HCl to make precipitate
tube8- cloudy blue
What were the results of the lipid section of the lab
Water formed 1 layer and the polar and nonpolar solute dissolved.
Water and corn oil formed 2 layers and the polar and nonpolar solutes would dissolve.
Corn oil and olive oil formed 1 layer which was an orange color and the polar and nonpolar solutes would dissolve.
what if there is no oil mixed with sudan red
sudan red can somewhat dissolve in the water
why was dishwashing detergent used for DNA extraction
it breaks the cell membrane
why was EDTA used for DNA extraction and how does it work
It protects the DNA from DNase by removing Mg and Ca from solution so iron can’t be available to DNase
why was ethanol used in DNA extraction
it principates the DNA so it will come out
what was the purpose of using the biuret reagent in protein section of the lab
this reagent detects peptide bonds, and proteins are held together by peptide bonds. When the reagent finds a peptide bond, it will turn purple
what were the results of the protein section of the lab
The milk and egg whites turned purple so they were the proteins and contained peptide bonds
what temp do most enzymes work at
37 degrees C (human body temp)
what are the function of cofactors
not enzymes; ionic or organic; bind to active site and make enzyme active
what are the 2 types of inhbitors
- comparative
- noncomparative
what is a comparative inhibitor
binds with active site and compete with substrate so you have to add more substrate in order for substrate ti compete better
what is a noncomparative inhibitor
bind to a location other than active site so substrate won’t bind anymore and substrate will have to be restored. Noncomparative changes shape of enzyme
