Biol 445 final material

Question 1

MDM2

Answer 1

and E3 ubiquitinase that destroys p53

Question 2

p53: tumor suppressor or oncogene?

Answer 2

tumor suppressor

Question 3

E2F’s effect on cyclin E

Answer 3

produces more of it

Question 4

Cyclin E

Answer 4

hyperphosphorylates Rb later (CDK2)

Question 5

Cyclin D

Answer 5

hypophosphorylates Rb early on (CDK 4/6)

Question 6

Hyper-phosphorylated Rb

Answer 6

is free from E2F

recruits histone acetylase

Question 7

Un/Hypo-phosphorylated Rb

Answer 7

stays bound to E2F

recruits histone deacetylaces

Question 8

Rb regulates

Answer 8

progression through G1

Question 9

biological role of abl

Answer 9

phosphorylates focal adhesion proteins and regulators of the actin cytoskeleton
responds to DNA damage

Question 10

homologous abl

Answer 10

arg

Question 11

abl structure is similar to?

Answer 11

src

Question 12

Oncogenic abl mutation (in what domain?)

Answer 12

Myristate; carboxy terminus

Allows for “off” formation

Question 13

bcr/abl fusion protein effects

Answer 13

increased proliferation/differentiation
increased stem cell turnover
decreased apoptosis
creates cytoskeletal/adhesion effects

Question 14

Philadelphia chromosome

Answer 14

t9;22 causes CML and present in some adult ALLs

fuses bcr and abl genes

Question 15

TCF4

Answer 15

turns Wnt pathway off
used by Clevers to find genes turned on by Wnt are in crypts
and genes turned off by Wnt are in villi

Question 16

TCF

Answer 16

together with armadillo activates Wnt-responsive genes

Question 17

armadillo

Answer 17

normally is phosphorylated by Fz

without phosphorylation, moves to nucleus and binds to TCF

Question 18

wingless (wnt)

Answer 18

induces segmentation
places of expression take on anterior fate
binds Fz

Question 19

p95 is also known as

Answer 19

beta-catenin; hedgehog

Question 20

FAP genetic cause

Answer 20

APC gene

Question 21

apoptosome

Answer 21

activates caspases

Question 22

apaf bound to cytochrome-c:

Answer 22

assembles apoptosome

Question 23

cytochrome-c

Answer 23

binds to apaf

Question 24

bcl-2 causes:

Answer 24

cytochrome-c to leave mitochondria membrane

opens pores in mitochondrial membrane

Question 25

bcl-2 equivalent to

Answer 25

ced-9

Question 26

ced-3

Answer 26

results in apoptosis

Question 27

ced-4

Answer 27

activates ced-3

Question 28

ced-9

Answer 28

inhibits ced-4

Question 29

p21 function

Answer 29

Forms inhibitory complex between CDK2 and cyclin E

Results in G1 arrest

Question 30

Cell cycle arrest target of p53

Answer 30

p21

Question 31

p53 effect on Bcl-2

Answer 31

Inhibition

Question 32

ARF

Answer 32

Binds and sequesters MDM2

Question 33

E2F

Answer 33

Hyperproliferative stress response
Active when the cell cycle is active
Activates ARF

Question 34

Chk2, Chk1

Answer 34

Phosphorylate both MDM2 and p53

Prevent p53 degradation

Question 35

Rad9

Answer 35

Activates Chk1

Question 36

ATR

Answer 36

Genotoxic stress response
Activates in response to replication errors and single stranded DNA breaks
Phosphorylates Rad9

Question 37

ATM

Answer 37

Genotoxic stress response
Activates in response to double stranded, major breaks
Activates Chk2