BIOL 202 2nd Lab Exam

Question 1

API strips (What do they do?)

Answer 1

API strips: they test to analyze the biochemical and enzymatic capabilities of a bacterium.

Question 2

Biochemical tests (What are they?)

Answer 2

Biochemical tests: determine a bacterium’s identity and capabilities

Question 3

IVMiC (What does it stand for?)

Answer 3

IMViC tests: analyzes Indole Methyl-red, Vogues Pasteur, and citrate utilization

Question 4

Indole test (What does it test for?)

Answer 4

○ Indole test: determines if an organism can break down tryptophan and produce indole as a by-product
§ Red: positive test (breaks down tryptophan)
§ Yellow: negative test (no breakdown)

Question 5

Indole test (What do the results mean?)

Answer 5

○ Indole test: determines if an organism can break down tryptophan and produce indole as a by-product
§ Red: positive test (breaks down tryptophan)
§ Yellow: negative test (no breakdown)

Question 6

What does methyl-red test for?

Answer 6

§ Methyl-red Positive: organism can break down glucose and produce 2,3 butanediol
□ Red: positive test (break down glucose)
- Yellow: negative test (cant break down glucose)

Question 7

Methyl-red (What do the two colours mean?)

Answer 7

Methyl-red Positive: organism can break down glucose and produce 2,3 butanediol
□ Red: positive test (break down glucose)
- Yellow: negative test (cant break down glucose)

Question 8

What does a VP test test for

Answer 8

§ VP positive: has acetoin as an end product
□ Red: positive test (acetoin is produced)
- Clear: negative test (no acetoin)

Question 9

What do the colours of VP mean

Answer 9

§ VP positive: has acetoin as an end product
□ Red: positive test (acetoin is produced)
- Clear: negative test (no acetoin)

Question 10

Can you have both VP and Methyl positive?

Answer 10

NO, IT IS USUALLY ONLY METHYL-RED OR VP NOT BOTH

Question 11

Citrate test (What does it test for?)

Answer 11

○ Citrate: whether the culture can ferment citrate as a sugar source
§ Green: negative test (can’t ferment citrate)
§ Blue: positive test (can ferment citrate)

Question 12

Citrate (What does what do the colours mean?)

Answer 12

○ Citrate: whether the culture can ferment citrate as a sugar source
§ Green: negative test (can’t ferment citrate)
§ Blue: positive test (can ferment citrate)

Question 13

Catalase test (What does it test for?)

Answer 13

Catalase test: determines the presence of enzyme catalase

Question 14

Catalase test (What does the reaction mean?)

Answer 14

○ We will use hydrogen peroxide for this test
§ If they produce bubbles: they have catalase
§ If they don’t: they don’t have catalase