Bio145 quiz 5 (ch 8-9) Flashcards
Which of the following is NOT a goal of recombinant DNA technology?
A) Eliminating undesirable traits from livestock or crops
B) Creating organisms capable of producing useful products
C) Making it possible to clone humans
D) Combining genetic material from more than one organism to produce new useful organisms
C) Making it possible to clone humans
Restriction enzymes cut DNA at specific nucleotide sequences and may generate ____ or _____ ends.
a) Blunt, sticky
b) DNA, RNA
c) Point, gross
d) Southern, Northern
A) blunt, sticky
Injecting DNA into cells can be accomplished using ____________
a) Silicon chips
b) Micropipettes, or compressed air and gold beads
c) Nucleic acids
d) Nitrocellulose membranes
b) micropipettes, or compressed air and gold beads
The following product(s) are possible due to biotechnology:
a) Egg-free vaccine
b) Clotting factor
c) Human growth hormone
d) All of the above
d) All of the above
Vectors deliver genes to cells and can be in what three forms?
a) Replication, transcription, and translation
b) Viral genomes, transposons, and plasmids
c) Northern blot, Southern blot, DNA microarray
d) Larry, Curly, Moe
B) viral genomes, transposons, and plasmids
The process of amplifying (making multiple copies) DNA without cells used in biotech applications?
a) Gel electrophoresis
b) Protoplast fusion
c) PCR (polymerase chain reaction)
d) Southern blot
C) PCR (polymerase chain reaction)
Which of the following is NOT an example of medical applications of recombinant DNA technology?
a) Protein synthesis of insulin
b) Generating antigen vaccines
c) Gene therapy to cure blindness
d) Herbicide resistance
d) Herbicide resistance
Which of the following statements is TRUE of disinfectants?
a) They are used on living tissue
b) They are used on inanimate surfaces
c) They are dangerous due to potential explosion
d) They are only effective for short periods of time (seconds to minutes)
B) They are used on inanimate surfaces
Which is an example of antisepsis?
a) Heat used to kill potential pathogens in apple juice
b) Surgeon washes her hands before surgery
c) A nurse prepares an injection site with an alcohol swab
d) A public toilet is treated with disinfectants
C) a nurse prepares an injection site with an alcohol swab
Silvadene, a topical treatment for burns, contains 1% silver. What category of chemical control agents is silvadene?
a) Heavy metals
b) Halogens
c) Surfactants
d) Aldehydes
A) Heavy metals
Disinfecting agents naturally produced by microorganisms are____________
a) Aldehydes
b) Quats
c) Antibiotics
d) Halogens
C) Antibiotics
The process of filtration is a(n) ______
a) Ineffective method for removing microbes
b) Antiseptic procedure
c) Disinfectant method
d) Sterilizing method
D) sterilizing method
Which of the following is NOT an antimicrobial method using moist heat?
a) Autoclaving
b) Boiling
c) Ultra-high heat sterilization
d) Dessication
d) dessication
The use of lysozyme during the preparation of cheese and wine is effective in reducing the numbers of ______.
a) Prions
b) Bacteria
c) Fungi
d) Viruses
B) Bacteria
Which of the following procedures is currently the standard test in the U.S. for evaluating the efficiency of antiseptics and disinfectants, and includes a step-wise process of using a metal cylinder dipped in a bacterial culture, then disinfectant, rinsed, and finally dipped in nutrient media.
a) Kelsey-Sykes Capacity test
b) Phenol coefficient
c) Thermal death point
d) Use-dilution test
D) use-dilution test
