BIO LAB Flashcards
hypotheses
a possible answer to the question being asked
null hypothesis (indicated by H knot-0)
assumes that the experimental treatment will have no effect
experiments are conducted to ______ the experiment
test
alternate hypothesis (indicated by H-A)
states there is a significant difference between the control and experimental group
independent variable
the variable that is manipulated (cost)
dependent variable
the measured result of the manipulation (effect)
experimental treatment
examines the effect of the independent variable on the dependent variable
control treatment
examines the possible effect of other factors besides the manipulated variable on the dependent variable
constants
factors that are kept the same in each treatment
replication
performing the experiment under the same conditions many times to attain reliable results
sample mean
average of a sample
population mean
average of all individuals in a species in entire population
theory
established after a hypothesis has failed to be rejected multiple times
enzymes
biological catalysts that increase the rates of biochemical reactions in cells by facilitating the conversion of a specific substrate to a product
enzymes increase rate of reaction by lowering:
activation energy required for reaction to occur
substrates
reactants
enzyme kinetics
involves studying how fast an enzyme converts substrate to products
enzyme activity
the rate or velocity of the reaction catalyzed by the enzyme
spectrophotometer
measures concentration of pigment molecules by measuring how much light is absorbed as the reaction occurred
transmittance (T)
the ratio of transmitted light to incident light
absorbance (A)
the negative log of transmittance
Vmax
the maximum rate of reaction for a given enzyme and substrate
Km
the substrate concentration at which the reaction rate is half of its maximum value
competitive inhibitors
similar in shape to an enzyme’s substrate and compete with the substrate for binding to the active site
non-competitive inhibitors
does not compete with the substrate for the active site, binds instead to other sites on the enzyme
eutrophication
the process by which a water body becomes enriched in nutrients from terrestrial ecosystems and can happen by natural processes and human activities
photolysis
the splitting of water into protons and oxygen in the presence of light