8.2 The Calvin Cycle Flashcards
the Calvin cycle consists of:
15 reactions that synthesize carbohydrates from CO2 (can be grouped into three main steps)
what is the first step in the Calvin cycle?
CARBOXYLATION-carbon dioxide absorbed from the air is added to a 5-carbon molecule (RuBP), catalyzed by the enzyme rubisco
what is the second step in the Calvin cycle?
REDUCTION-energy and electrons are transferred to the compounds formed from carboxylation, NADP transfers two electrons and one proton; inorganic phosphate and NADP+ is released
what is the third step in the Calvin cycle?
REGENERATION-of the 5-carbon molecule needed for carboxylation, 3-carbon compounds are reorganized and combined to produce RuBP
ribulose 1,5-bisphosphate (RuBP)
the 5-carbon sugar to which carbon dioxide is added by the enzyme rubisco
ribulose bisphosphate carboxylase oxygenase (rubisco)
the enzyme that catalyzes the carboxylation reaction in the Calvin cycle
what do you call an enzyme that adds carbon dioxide to another molecule?
carboxylase
3-phosphogylcerate (3-PGA)
a 3-carbon molecule; two molecules of 3-PGA are the first stable products of the Calvin cycle
what is the reducing agent of the Calvin cycle?
NADPH (a cofactor/coenzyme)-it transfers the electrons that allow carbohydrates to be synthesized from CO2
NADPH can move freely within the stroma of the chloroplast buy only transfers energy/electrons under:
catalysis of a specific enzyme
the reduction of 3-PGA involves two steps:
1) ATP donates a phosphate group to 3-PGA
2) NADPH transfers two electrons plus one proton to the phosphorylated compound which releases one phosphate groups
one molecule of carbon dioxide-3 molecules of 3-PGA which requires:
2 ATP and two NADPH for each molecule of carbon dioxide incorporated by rubisco
ATP plays an essential role in preparing:
3-PGA for the addition of energy and electrons from NADPH
what provides most of the energy incorporated in the bonds of the carbohydrate molecules?
NADPH
triose phosphate
a 3-carbon carbohydrate molecule, produced by the Calvin cycle and exported from the chloroplast, starting point for synthesis of larger sugars ex. glucose and sucrose
most triose phosphate molecules are used to:
regenerate RuBP
for every six triose phosphate molecules produced, how many can be withdrawn from the Calvin cycle?
only one
twelve reactions of the Calvin cycle occur in which step?
the last step: regeneration of RuBP
what is required for the regeneration of RuBP?
ATP
what is the total energy requirements of the Calvin cycle for each molecule of carbon dioxide incorporated by rubisco?
2 NADPH and 3 ATP
during regeneration, five 3-carbon triose phosphate molecules turns into:
three 5-carbon RuBP molecules
the Calvin cycle does not use:
sunlight directly - referred to the light independent or dark reactions of photosynthesis
in a photosynthetic cell, the Calvin cycle occurs only in the light because:
NADPH and ATP are supplied by the photosynthetic electron transport chain and several cofactors are activated by the transport chain too
how were the steps of the Calvin cycle determined?
through the use of radioactive carbon dioxide
the Calvin cycle is capable of producing more carbohydrates than the cells need which is dangers because:
if carbohydrates accumulate in the cell, they cause water to enter the cell by osmosis, which might damage the cell
excess carbohydrates are converted to:
starch