BC2 Flashcards
Most Common Site for Venipuncture
Antecubital Fossa (Median, Cephalic, Basilic)
Cleansing The Site Motion
Friction of Back & Forth
Angle Between Skin & Needle
<30 Deg
Length of Time of torniquet Application
< 1 Minute/60 Secs
Cause of Spx Hemolysis
Prolonged torniquet
Moisture/Contamination in Blood Tube
Needles with too Small Bores
Excessive Agitation
Frothing (Bubbles) in Sample
Effects of Prolonged torniquet Application (Hhs)
Hemolysis
Hemoconcentration
Shortened Coagulation Time
Condition Which Venous Flow is Slowed
Stasis
Local Accumulation of FVIII & VWF Results in
False Shortening of Coagulation Test
Distance of torniquet
3-4 inches or 7.5 to 10 cm
Most Common Needle Size for Adult Venipuncture
21 G ; 1 inch Length
Phlebotomist Should Never Puncture More Than
Twice
When to Replace Sharp Container
No More Than Three Quarters Full
Most Common Cause of A Needle Puncture
Improper Disposal
Usual EDTA Used Component
Dipotassium (K2)
CBC is Also Called
Hemogram
Optimal Anticoagulant Conc for Hema
1.5 Mg/mL of Blood
Blood Spx for CBC Must Be Analyzed within
6 hours of Collection (Room Temperature Storage); 24 hours if Ref Temperature
Blood Smear Should Be Made within
3 hours
EDTA Prevents Platelet
Aggregation
EDTA Causes Swelling of Platelets That Approximately increases for
20% During The First Hour
Excessive EDTA Causes
Decreased Hct, ESR, Degenerate Changes in WBC, increased MCHC
RBC Parameters
RBC Count, Hgb, Hct, RBC indices, RDW, Retic Count
WBC Parameters
WBC Count, Neut, Lymph, Mono, Eo, Baso (Relative & Absolute Count)
Platelet Parameters
Platelet Count; Mean Platelet Volume
Formation of Ring-Shaped Molecular Complex in Which Metal Ions is Covalently Bound
Chelation
Process in Which A Current Test Result is Compared with The Result of The Same Test From The Previous Specimen From The Same Patient
Delta Check
Delta Check Method of QC
Utilizes The Patient’s Own Data to Monitor Population Values
Test Being Ordered Automatically Based On The Results of Prior Tests or Preset Parameters
Reflex Test
Any Numerical Value That Depicts An Entire Population
Parameter
Heparin Anticoagulant is Used for
Flow Cytometry, Plasma Chem, Blood Gas Studies
Optimal Anticoagulant Conc for Heparin
15-20 Units/mL Blood
Actions of Heparin
Binds Anti-Thrombin/inhibits Thrombin & Factor Xa
Heparin That Causes Least Interference in Chemistry Testing
Lithium Heparin
Heparin Causes Cellular Clumping (Especially of Platelets), Which Leads to
Pseudoleukocytosis (Falsely High WBC Count) & Pseudothrombocytopenia (Falsely Low Platelet Count)
Heparin Causes in Blood Smears
Bluish Coloration of The Background On Blood Smears Stained with A Romanowsky Stain Because of Its pH
Critical Ratio for 3.2% Sodium Citrate Between Anticoagulation & Blood
1:9
Forceful Mixing or An Excessive Number of Inversions Can Do What in Sodium Citrate
Activate Platelets & Shorten Clotting Times
Factors That Affect Coagulation Results; Shortened Coagulation Result (Heparin)
Hemolysis, Excessive Agitation, Prolonged torniquet Application, Excessive Needle Manipulation, Platelet Contamination
Prolonged Coagulation is Due to
Presence of Clots, increased Acidity Concentration
Invert Each Tube Containing Additive
Immediately
Only Blood Culture Tubes, Glass Nonadditive Serum Tubes, or Plastic Serum Tubes without A Clot Activator May Be Collected Before The
Coagulation Tube
Black Top Anticoagulant for Westergren
3.8% Na Citrate (Blood 4:1)
Pink top is Used for What Section
Blood Bank Tests (WB, Hema Determination)
Light Blue May Contain Either
3.2% Na Citrate or CTAD (Citrate, Theophylline, Adenosine, Dipyridamole)
CTAD Uses
Coagulation Test, Platelet Function Assay (PF4 & B-Thromboglobulin Assays)