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Somatosensory System > Bacterial Growth Metabolism Visser > Flashcards

Bacterial Growth Metabolism Visser Flashcards

1
Q

How is bacterial growth Measured ?

A

Direct Counting
- Microscopy
- Total Numbers
Colony Forming Units
- Serial dilution and plate on agar media
- Viable cell count
Turbidity
- Measure Light absorption

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2
Q

Bacteria require Macronutrients and Micronutrients

A

Macronutrients
- Large amounts
- Nitrogen, hydrogen, carbon, ect.
- Structural or functional role in cells

Micronutrients
- Trace amounts
- Zinc, Cobalt, Molybdenum
- Used as cofactors in enzymatic reactions

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3
Q

Prototroph

A

bacteria derives all nutrient requirements from sources contained in a mixture of macronutrients and micronutrients
(E. coli)

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4
Q

Fastidious Bacteria

A

have complex and specialized nutrient requirement (obligate pathogens)
- Ex need vitamins and A.A’s
(Mycobacterium Tuberculosis and Staphylococcus Aureus)

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5
Q

Most identified bacteria grow on artificial media

A

5% identified and cultured

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6
Q

Obligate pathogens cannot be (or easily grown) in culture

A

Treponema pallidum requires growth in mammalian hosts

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7
Q

Some bacteria have to be cultured intracellularly

A

Rickettsia(R.typhi (typhus), R.Rickettsii (spotted Fever)

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8
Q

Complex growth medium

A

Undefined mixture
BHI broth for S.aureus

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9
Q

Defined growth medium

A

defined composition and concentration of nutrients
ex. M9 for E.coli

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10
Q

Bacteria growing in defined media are able to synthesize macromolecules

A

Bacteria growing in complex media are unable to synthesize macromolecules, and obtains them from the media

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11
Q

Selective media

A

Encourages growth of some and discourages growth of others

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12
Q

Differential media

A

Causes an observable change (biochemical change)pH, color.
- Differentiate colonies (species)

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13
Q

Obligate Aerobe

A

Pseudomonos Aeruginosa

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14
Q

Microaerophile

A

Campylobacter fetus

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15
Q

Facultative Anaerobe

A

Streptococcus gordonii

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16
Q

Obligate anaerobe

A

Porphyromonas gingivalis, oral spirochetes (many dental pathogens)

17
Q

Halophiles

A

Extreme Saltwater 3%

human saline concentration (0.85%)

18
Q

Cheeks, Lips, Palate (Environment)

A

-Desquamation
-Saliva a major influence
-Microflora has low diversity
-Facultative anaerobes
-Streptococcus spp. predominate.
- Some periodontal pathogens persist by invading buccal cells

19
Q

Teeth (environment)

A
  • Non shredding surface
  • Stagnant Sites; Food impaction possible
  • Diverse microfora; Site variation
    -Many obligate anaerobes
    -Influenced by saliva and GCF
  • Streptococcus, Actinomyces, Veilonella,Fusobacterium,Prevotella,Treponema, unculturable organisms.
20
Q

Tongue (environment )

A
  • Highly papillate surface
  • Some anaerobic sites
  • Desquamation
  • Diverse microflora
  • Facultative and Obligate anaerobes
  • Streptococcus, Actinomyces, Rothia, Neisseria, some gram negative anaerobes
21
Q

Mouth 35-36C
Healthy gingiva sulcus= 33-36C
Periodontal disease=39C

A

Palate 7.34 , buccal 6.3

Gingival Crevice 6.9
Disease=7.4 plus

Caries-acidic

22
Q

Aerobic Respiration

A

O2 is terminal e- acceptor

23
Q

Anaerobic Respiration

A

e- acceptor is NOT O2 (inorganic molecules ex. NO3,NO2)

24
Q

Fermentation

A

NO O2 inorganic e- acceptor
- Use organic compounds as e- acceptor

25
Q

Aerobic Respiration

A

CO2 H2O & 38ATP

26
Q

Anaerobic Resp.

A

Inorganic Compounds + 30ATP

27
Q

Fermentation

A

Organic Products + 4 ATP

28
Q

Special Cases

A

Some bacteria synthesize cellulose
synthesis peptidoglycan, LPS, Capsules etc.

29
Q

Sugar metabolism

A

Oral Streptococcus

30
Q

Polysaccharide Production

A

Glucosyltransferase (GTF)
- Extracellular -(biofilm production)
- Intracellular - Glycans and storage molecules

31
Q

Unique Proteolytic Properties

A
  • Novel Nutrients (hemoglobin-heme, transferrin)
  • P.gingivalis: gingipains
32
Q

Production of sulfur Compounds

A
  • P.gingivalis, T.denticola
  • Sulfur amino Acids (cyst, met)

Somatosensory System (24 decks)

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