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BACTERIOLOGY > B2.3 GROUP B,C and G STREPTOCOCCI > Flashcards

B2.3 GROUP B,C and G STREPTOCOCCI Flashcards

1
Q

GROUP B STREPTOCOCCI

A

Streptococcus agalactiae

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2
Q

have group B–specific antigen, acid-stable
polysaccharide located in cell wall

normal flora of female genital tract and lower
gastrointestinal tract

A

Streptococcus agalactiae

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3
Q

Streptococcus agalactiae MOT

 Endogenous strain

A

gaining access to sterile site(s) probable

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4
Q

Streptococcus agalactiae MOT normally

A

Direct contact

 Causes infection of fetus during passage through the colonized birth canal and premature rupture of mother’s membrane

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5
Q

VIRULENCE FACTORS of Streptococcus agalactiae

A

CAPSULE

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6
Q

 important virulence factor for S. agalactiae
 prevents phagocytosis but is ineffective after
opsonization

A

CAPSULE

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7
Q

 most significant component of the capsule of S. agalactiae
critical virulence determinant

A

SIALIC ACID

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8
Q

AVIRULENT FACTORS of S. agalactiae

A

Hemolysin
CAMP factor
Neuraminidase
Dnase
Hyaluronidase
Protease

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9
Q

RELATED INFECTIONS AND DISEASES with S. agalactiae

A
  1. NEONATAL SEPSIS and MENINGITIS
  2. Pneumonia
  3. Postpartum Infection ENDOMETRITIS
  4. Osteomyelitis
  5. UTI
  6. Puerperal Infection
  7. Endocarditis (Tricuspid Valve Endocarditis)
  8. Skin infection
  9. Important etiologic agent of bovine mastitis
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10
Q

S. agalactiae is the most common etiologic cause of

A

NEONATAL SEPSIS and
MENINGITIS

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11
Q

Neonatal GBS Diseases
Early-onset infection (<7 days old) causes

A

pneumonia and sepsis

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12
Q

Neonatal GBS Disease
Late-onset infection n (7 days old - 3 months old) causes

A

meningitis and
sepsis

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13
Q

Specimen of choice for determining S. agalactiae

A

Vaginal and Rectal swabs

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14
Q

LABORATORY DIAGNOSIS methods for S. agalactiae

A
  1. Culture Medium
  2. CHRISTIE, ATKINS, AND MUNCH-PETERSEN (CAMP) TES
  3. HIPPURATE HYDROLYSIS TEST
  4. SEROTYPING
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15
Q
  1. Culture Medium methods for S. agalactiae
A

Blood agar plate
Selective broth
Granada Agar

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16
Q

Types of selective broth for S. agalactiae

A

a. Lim Broth
b. TransVag broth
c. StrepB Carrot Broth

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17
Q

Blood agar plate results for S. agalactiae

A

grayish white, mucoid, more translucent to opaque, soft, smooth colonies surrounded by smaller zone of βhemolysis

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18
Q

Lim Broth components for S. agalactiae

A

Todd-Hewitt broth with Colistin and
Nalidixic acid = TRANSPORT MEDIUM

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19
Q

TransVag broth components for S. agalactiae

A

Gentamicin and Nalidixic acid

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20
Q

StrepB Carrot Broth method for S. agalactiae

A

orange or red pigment (6 hours
incubation)

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21
Q

 selective agar for vaginal or rectal swabs
yellow to orange colonies produced by S. agalactiae

A

Granada Agar

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22
Q

Test for presumptive identification of GBS: S. agalactiae (+)

A

CHRISTIE, ATKINS, AND MUNCH-PETERSEN (CAMP) TEST

23
Q

act synergistically with β-hemolysin produced by S. aureus to cause enhanced lysis of RBC

A

CAMP FACTOR

24
Q

CHRISTIE, ATKINS, AND MUNCH-PETERSEN (CAMP) TEST method

A

GBS are streaked perpendicular to a streak of S. aureus on
sheep blood agar

25
Q

CHRISTIE, ATKINS, AND MUNCH-PETERSEN (CAMP) TEST positive result for S. agalactiae

A

Arrowhead-shaped hemolysis

26
Q

RAPID CAMP TEST OR SPOT CAMP TEST method

A

place a drop of extracted β-lysin on area of confluent growth of suspected GBS

 incubation: 35° C for 20 minutes

27
Q

RAPID CAMP TEST OR SPOT CAMP TEST positive result for S. agalactiae

A

enhanced hemolysis

28
Q

S. agalactiae (+) has ______
that hydrolyzes sodium hippurate to form sodium benzoate and glycine

A

hippuricase (hippurate hydrolase)

29
Q

Ammonia + ninhydrin and hydrindantin will show what color

A

PURPLECOLORED COMPLEX

30
Q

Test that detects S. agalactiae by reacting ammonia with the products of deaminated glycine as well as reduced form of ninhydrin which causes a purple colored complex when positive

A

HIPPURATE HYDROLYSIS TEST

31
Q

Coagglutination or Latex Agglutination method of detecting S. agalactiae

A

SEROTYPING

32
Q

S. agalactiae treatment

A

PENICILLIN

33
Q

GROUPS C AND G STREPTOCOCCI are recovered from

A

the upper respiratory tract, vagina and skin

34
Q

are animal pathogens and the
main source of STREPTOKINASE

A

Group C streptococci

35
Q

GROUPS C AND G STREPTOCOCCI
spp.

A

S. dysagalactiae subsp. equisimilis and
S. equi subsp. zooepidemicus

36
Q

Types of Group C and G Streptococci

A

Large Colony-forming Isolates
Small-Colony–forming Isolates

37
Q

isolates with groups A, C, G and L antigens belong to the

A

pyogenic streptococci

38
Q

large-colony–forming β-hemolytic isolates with group C and G antigens belong to

A

S. dysgalactiae subsp. equisimilis

39
Q

Large Colony-forming Isolates

A

pyogenic streptococci
S. dysgalactiae subsp. equisimilis

40
Q

small-colony–forming β-hemolytic isolates with group C and G antigens belong to the

A

S. anginosus group

41
Q

Small-Colony–forming Isolates

A

S. anginosus group

42
Q

Group C Streptococci

A

all species (S. equi, S. equisimilis, S. zooepidemicus) are β-hemolytic except

S. dysagalctiae which may be αhemolytic or non-hemolytic

43
Q

Group C Streptococci are differentiated by

A

carbohydrate fermentation

44
Q

RELATED INFECTIONS AND DISEASES of GCS and GGS

A

animal pathogens but may infect humans

45
Q

causes disease in horse

46
Q

may cause pharyngitis, puerperal sepsis, endocarditis, bacteremia, osteomyelitis, brain abcess, post-operative wound infection and pneumonia in humans

A

S. equisimilis

47
Q

source of streptokinase used in Thrombolytic Therapy

A

S. equisimilis

48
Q

S. equisimilis may cause what diseases in humans

A

pharyngitis,
puerperal sepsis,
endocarditis,
bacteremia,
osteomyelitis,
brain abcess,
post-operative wound infection and pneumonia

49
Q

 similar types of acute infections described for S. pyogenes and S. agalactiae, but usually involve compromised patients

A

Group C and G Streptococci

50
Q

occur in patients with underlying malignancies

A

Group G streptococci

51
Q

occasionally have been associated with acute pharyngitis

A

Group C organisms

52
Q

Group C streptococci are susceptible to what antimicrobials

A

Bacitracin and SXT

53
Q

Group G streptococci may be resistant or susceptible to what antimicrobial

A

bacitracin

BACTERIOLOGY (32 decks)

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