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BACTERIOLOGY > B2.3 GROUP B,C and G STREPTOCOCCI > Flashcards

B2.3 GROUP B,C and G STREPTOCOCCI Flashcards

1
Q

GROUP B STREPTOCOCCI

A

Streptococcus agalactiae

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2
Q

have group B–specific antigen, acid-stable
polysaccharide located in cell wall

normal flora of female genital tract and lower
gastrointestinal tract

A

Streptococcus agalactiae

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3
Q

Streptococcus agalactiae MOT

 Endogenous strain

A

gaining access to sterile site(s) probable

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4
Q

Streptococcus agalactiae MOT normally

A

Direct contact

 Causes infection of fetus during passage through the colonized birth canal and premature rupture of mother’s membrane

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5
Q

VIRULENCE FACTORS of Streptococcus agalactiae

A

CAPSULE

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6
Q

 important virulence factor for S. agalactiae
 prevents phagocytosis but is ineffective after
opsonization

A

CAPSULE

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7
Q

 most significant component of the capsule of S. agalactiae
critical virulence determinant

A

SIALIC ACID

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8
Q

AVIRULENT FACTORS of S. agalactiae

A

Hemolysin
CAMP factor
Neuraminidase
Dnase
Hyaluronidase
Protease

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9
Q

RELATED INFECTIONS AND DISEASES with S. agalactiae

A
  1. NEONATAL SEPSIS and MENINGITIS
  2. Pneumonia
  3. Postpartum Infection ENDOMETRITIS
  4. Osteomyelitis
  5. UTI
  6. Puerperal Infection
  7. Endocarditis (Tricuspid Valve Endocarditis)
  8. Skin infection
  9. Important etiologic agent of bovine mastitis
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10
Q

S. agalactiae is the most common etiologic cause of

A

NEONATAL SEPSIS and
MENINGITIS

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11
Q

Neonatal GBS Diseases
Early-onset infection (<7 days old) causes

A

pneumonia and sepsis

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12
Q

Neonatal GBS Disease
Late-onset infection n (7 days old - 3 months old) causes

A

meningitis and
sepsis

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13
Q

Specimen of choice for determining S. agalactiae

A

Vaginal and Rectal swabs

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14
Q

LABORATORY DIAGNOSIS methods for S. agalactiae

A
  1. Culture Medium
  2. CHRISTIE, ATKINS, AND MUNCH-PETERSEN (CAMP) TES
  3. HIPPURATE HYDROLYSIS TEST
  4. SEROTYPING
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15
Q
  1. Culture Medium methods for S. agalactiae
A

Blood agar plate
Selective broth
Granada Agar

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16
Q

Types of selective broth for S. agalactiae

A

a. Lim Broth
b. TransVag broth
c. StrepB Carrot Broth

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17
Q

Blood agar plate results for S. agalactiae

A

grayish white, mucoid, more translucent to opaque, soft, smooth colonies surrounded by smaller zone of βhemolysis

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18
Q

Lim Broth components for S. agalactiae

A

Todd-Hewitt broth with Colistin and
Nalidixic acid = TRANSPORT MEDIUM

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19
Q

TransVag broth components for S. agalactiae

A

Gentamicin and Nalidixic acid

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20
Q

StrepB Carrot Broth method for S. agalactiae

A

orange or red pigment (6 hours
incubation)

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21
Q

 selective agar for vaginal or rectal swabs
yellow to orange colonies produced by S. agalactiae

A

Granada Agar

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22
Q

Test for presumptive identification of GBS: S. agalactiae (+)

A

CHRISTIE, ATKINS, AND MUNCH-PETERSEN (CAMP) TEST

23
Q

act synergistically with β-hemolysin produced by S. aureus to cause enhanced lysis of RBC

A

CAMP FACTOR

24
Q

CHRISTIE, ATKINS, AND MUNCH-PETERSEN (CAMP) TEST method

A

GBS are streaked perpendicular to a streak of S. aureus on
sheep blood agar

25
CHRISTIE, ATKINS, AND MUNCH-PETERSEN (CAMP) TEST positive result for S. agalactiae
Arrowhead-shaped hemolysis
26
RAPID CAMP TEST OR SPOT CAMP TEST method
place a drop of extracted β-lysin on area of confluent growth of suspected GBS  incubation: 35° C for 20 minutes
27
RAPID CAMP TEST OR SPOT CAMP TEST positive result for S. agalactiae
enhanced hemolysis
28
S. agalactiae (+) has ______ that hydrolyzes sodium hippurate to form sodium benzoate and glycine
hippuricase (hippurate hydrolase)
29
Ammonia + ninhydrin and hydrindantin will show what color
PURPLECOLORED COMPLEX
30
Test that detects S. agalactiae by reacting ammonia with the products of deaminated glycine as well as reduced form of ninhydrin which causes a purple colored complex when positive
HIPPURATE HYDROLYSIS TEST
31
Coagglutination or Latex Agglutination method of detecting S. agalactiae
SEROTYPING
32
S. agalactiae treatment
PENICILLIN
33
GROUPS C AND G STREPTOCOCCI are recovered from
the upper respiratory tract, vagina and skin
34
are animal pathogens and the main source of STREPTOKINASE
Group C streptococci
35
GROUPS C AND G STREPTOCOCCI spp.
S. dysagalactiae subsp. equisimilis and S. equi subsp. zooepidemicus
36
Types of Group C and G Streptococci
Large Colony-forming Isolates Small-Colony–forming Isolates
37
isolates with groups A, C, G and L antigens belong to the
pyogenic streptococci
38
large-colony–forming β-hemolytic isolates with group C and G antigens belong to
S. dysgalactiae subsp. equisimilis
39
Large Colony-forming Isolates
pyogenic streptococci S. dysgalactiae subsp. equisimilis
40
small-colony–forming β-hemolytic isolates with group C and G antigens belong to the
S. anginosus group
41
Small-Colony–forming Isolates
S. anginosus group
42
Group C Streptococci
all species (S. equi, S. equisimilis, S. zooepidemicus) are β-hemolytic except S. dysagalctiae which may be αhemolytic or non-hemolytic
43
Group C Streptococci are differentiated by
carbohydrate fermentation
44
RELATED INFECTIONS AND DISEASES of GCS and GGS
animal pathogens but may infect humans
45
causes disease in horse
S. equi
46
may cause pharyngitis, puerperal sepsis, endocarditis, bacteremia, osteomyelitis, brain abcess, post-operative wound infection and pneumonia in humans
S. equisimilis
47
source of streptokinase used in Thrombolytic Therapy
S. equisimilis
48
S. equisimilis may cause what diseases in humans
pharyngitis, puerperal sepsis, endocarditis, bacteremia, osteomyelitis, brain abcess, post-operative wound infection and pneumonia
49
 similar types of acute infections described for S. pyogenes and S. agalactiae, but usually involve compromised patients
Group C and G Streptococci
50
occur in patients with underlying malignancies
Group G streptococci
51
occasionally have been associated with acute pharyngitis
Group C organisms
52
Group C streptococci are susceptible to what antimicrobials
Bacitracin and SXT
53
Group G streptococci may be resistant or susceptible to what antimicrobial
bacitracin

BACTERIOLOGY (32 decks)

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