Aspergillum fumigatus- Point- of Care Diagnostics Flashcards
Lateral -flow technology
simple paper-based devices intended to detect the presence of a target analyte in liquid sample without the need for specialized and costly equipment,
when biomarker is present in good stream/ liquid
double line
the control line
will form even when biomarker not present
how does the control line work
anti-mouse control- which binds any unbound AB- picks up any loose antibody
describe later flow when antigen of interest is present
1) antigen with flow up the paper via capillary action and bind to labeled JF antibodies which are bound to immobilised JF5 Ab bound to the the membrane
2) all unbound labeled JF5 will bind to the control antibodies
3) 2 lines will be present
describe lateral- flow assay the antigen of interest is not present
1) no antigen binds to labeled JF5 Ab, therefore no antibodies will bind to the immobilised JF5 Ab- no line here
2) unbound antibodies will still bind with the control lines
3) 1 line will be present
Bronchoalveolar Lavage (BAL)
Bronchoscope down into lung Feeds another tube containing fluid down Fluid forced down into the lung Fluid forced back out You may see aspergillus sporalating within lung tissue Taken to path lab to be identified Information comes back
- not a nice procedure
when biomarkers have been detect in the blood
its too late- detect before invasive
Non-invasive antibody guided molecular imaging involve using
ImmunoPET/ MRI
radiologists look for
abnormalities in the lungs
- not specific at all to fungal infections
however a radio linked antibody could show
very specifically if IPA was in the lung tissue
Antibody has to be
humanised
- using a human antibody- CDR grafting
ImmunoPET/MRI
Immune-positron Emission Tomography/ Magnetic Resonance Imaging
example of Immuno-positron emission tomography/ MRI
- knock out mice immunity
- 24h later challenge mice with spore of IPA (into trachea)
- inject trace intotail
- in vivo and ex vivo distribution of (6Cu)-labeled antibody tracers
why is using antibodies and Immuno-positron emission tomography/ MRI good
non-invasive
very specific
no uptake into lungs of mice with other infections
