ANALYTICAL METHODS [LIQUID CHROMATOGRAPHY]

Question 1

This is based on the distribution of solutes between a liquid mobile phase and a stationary phase.

Answer 1

Liquid Chromatography

Question 2

This is the most widely used type of LC.

Answer 2

High Performance Liquid Chromatography (HPLC).

Question 3

HPLC makes use of the rapid (?) rest.

Answer 3

HbA1c

EDTA

Question 4

Used pressure for fast separations, controlled temperature, in-line detectors and gradient elution technique.

Answer 4

High Performance Liquid Chromatography

Question 5

Fractionation of drugs, hormones, lipids, carbohydrates, and proteins.

Answer 5

High Performance Liquid Chromatography

Question 6

Separation and quatitation of various hemoglobin associated with specific diseases.

Answer 6

High Performance Liquid Chromatorgaphy

Question 7

This is where the mobile phase is more polar than the stationary phase.

Answer 7

Reverse Phase HPLC

Question 8

What are the 5 separation mechanisms used in liquid chromatography?

Answer 8

• Gel Molecular Sieve
• Ion Exchange
• Partition
• Affinity
• Adsorption (Liquid-Solid)

Question 9

This separated the molecule based on differences in their size and shape.

Answer 9

Gel Molecular Sieve Chromatography

Question 10

This gel sieve is used for the separation of enzymes, antibodies, and proteins.

Answer 10

Hydrophilic Gel

Gel Filtration

Ex. Dextran and Agarose

Question 11

This gel sieve is used for the separation of triglycerides and fatty acids.

Answer 11

Hydrphobic Gel

Gel Permeation

Ex. Sephadex

Question 12

This is the exchange of sample ions and mobile-phase ions with the charged groups of the stationary phase.

Answer 12

Ion Exchange Chromatography

Question 13

This is used for the separation of amino acids, proteins, and nucleic acids depending on sign and ion charge density.

Answer 13

Ion Exchange Chromatography

Question 14

This is the separation of compounds based on their partitions between the liquid mobile and stationary phases are coated in a solid support.

Answer 14

Partition Chromatography

Question 15

This is for the separation of theraputic drugs and their metabolies.

Answer 15

Partition Chromatorgraphy

Question 16

This is uses immobilized chemical ligands as the stationary phase to separate few solutes from other unretained solutes.

Answer 16

Affinity Chromatography

Question 17

Used for the separation of lipoproteins, carbohydrates, and glycated hemoglobin.

Answer 17

Affinity Chromatography

Question 18

Also used to separate large quantities of proteins and antibodies for study.

Answer 18

Affinity Chromatography

Question 19

This separation is based on the differences between the adsorption and desoprtion of solutes at the surface of a solid particle.

Answer 19

Adsorption Chromatography

Question 20

Compounts are adsorbed to a solid support such as (?) or (?).

Answer 20

Silica or Alumina

Question 21

These are the bases of separation.

Answer 21

• Rate of Diffusion
• Solubility of Solute
• Nature of Solvent
• Molecular Size
• Ionic Attraction
• Selective Separation of Substances

Etc.