ANALYTICAL METHODS [ELECTROPHORESIS 2]

Question 1

Separating molecules via migration through a pH gradient.

Constantly use power.

Answer 1

Isoelectric Focusing

Question 2

This is achieved by adding acid to anodic area of the electrolyte cell and adding base to the cathode area.

Answer 2

pH Gradient

Question 3

This is idea for separating proteins with identical sizes but with different net charges.

Answer 3

Isoelectric Focusing

Question 4

Proteint move in the electric field until (?).

Answer 4

Until it reaches pH equal to the isoelectric point.

Question 5

What are the supporting media used in isoelectric focusing?

Answer 5

• Agarose Gel
• PAGE
• Cellulose Acetate

Question 6

These are the advantages of using isoelectric focusing.

Answer 6

• Resolve mixture of proteins
• Detect isoenzymes (ACP, CK, ALP)
• Identify genetic variation (alpha-1-antitrypsin)
• Detect CSF ogliclonal bonding

Question 7

This method makes use of sample molecules and are separated by electro-osmotic flow (EOF).

Answer 7

Capillary Electrophoresis

Question 8

Which ions emerge first and which ion moves at a slower rate?

Answer 8

Cations emerges first and Anions move more slowly.

Question 9

True or False: EOF and Ion movement goes at opposite directions.

Answer 9

False, EOF and Ion movement are in the same direction.

Question 10

What are the uses of capillary electrophoresis?

Answer 10

• Separation, quantitiation, and determination of molecular weight in proteint and peptides.
• Analysis of PCR products.
• Analysis of organic and inorganic substances and drugs.

Question 11

This method is used to separate, detect, and identify one or more proteins in a complex mixture.

Answer 11

Western Blotting