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Clinical Chemistry [Lecture] > ANALYTICAL METHODS [COLORIMETRY] > Flashcards

ANALYTICAL METHODS [COLORIMETRY] Flashcards

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1
Q

This subclass of colorimetry is used by a spectophotometry or filter photometry.

A

Photoelectric Colorimetry

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2
Q

The primary analytical utility of spectrophotometry or filter photometry is the (?) for purposes of measurement.

A

Isolation of Discreet Portions of Spectrum

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3
Q

What are the two types of measurement used in photoelectric colorimetry?

A

Spectrophotometric and Photometric Measurements

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4
Q

This is the measurement of light that is within a narrower wavelength.

A

Spectrophotometric Measurement

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5
Q

This is the measurement of light without consideration of wavelength.

A

Photometric Measurement

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6
Q

This machine involves the measurement of light transmitted by a solution to determine light concentration and the absorbing substance.

A

Spectrophotometer

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7
Q

This is the currelty used spectrophotometer in NU. It can read in the UV and Visible Light spectrum.

A

UV-VIS

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8
Q

This is the simplest type of absorption spectrophotometer.

A

Single-beam Spectophotometer

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9
Q

What are the two types of spectrophotometer?

A

Single-beam and Double-beam

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10
Q

This spectophotometer is designed to make oe measurement at one specified wavelength.

A

Single-beam Spectophotometer

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11
Q

This spectrophotometer splits light into 2 components.

A

Double-beam Spectrophotmeter

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12
Q

What are the two components of the double-beam spectrophotometer.

A

One beam through the sample, the other through a reference solution (standard) or a blank.

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13
Q

What does the additional beam do in a double-beam spectrophotometer.

A

Corrects the variation of light source intensity.

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14
Q

What are the two types of double-beam spectrophotometer?

A

Double-beam in Space and in Time

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15
Q

This double-beam spectrophotometer uses 2 photodetectors.

A

Double-beam in Space

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16
Q

What are the two photodetectors in a double-beam in space spectophotemeter?

A

Sample Beam and Reference Beam

17
Q

This double-beam spectrophotometer uses 1 photodetector.

A

Double-beam in Time

18
Q

Double-beam in Time passes light alternatively through (?) and then (?) using a chopper.

A

Sample Cuvet and Reference Cuvet.

Clinical Chemistry [Lecture] (14 decks)

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