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Clinical Chemistry [Lecture] > ANALYTICAL METHODS [FEP AND AAS] > Flashcards

ANALYTICAL METHODS [FEP AND AAS] Flashcards

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1
Q

This type of photometry measures the light emitted by a single atom nurned in a flame.

A

Flame Emission Photometry

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2
Q

What is the principle behind the FEP?

A

Electron excitation from low to high energy.

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3
Q

What is the light source in FEP and what additional component does it serve as?

A

Flame, serves as the cuvette.

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4
Q

What kind of photodetector is used in FEP?

A

Barrier Layer Cell

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5
Q

What is the internal standard used in FEP, and what is the reason for its use?

A

Lithium or Cesium, used in order to correct variations.

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6
Q

What is the method used in FEP?

A

Indirect Internal Standard Method

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7
Q

This photometry is mainly used to measure the excitement of ions such as Na and K.

A

Flame Emission Photometry

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8
Q

This spectrophotometry measures the light absorbed by atoms dissociated by heat.

A

Atomic Absorption Spectrophotometry

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9
Q

Explain the principle observed in AAS.

A

The element is not excited, but dissociated from the chemical bond, unionized, and grounded.

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10
Q

What is the light source used for the AAS?

A

Hollow Cathode or Electrodeless Dishcarge Lamp

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11
Q

What is the monochromator used for the AAS?

A

Diffraction Gratings and Prims

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12
Q

What is the photodetector used in AAS?

A

PM Tube

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13
Q

What are the possible interferences AAS can have?

A

Chemical, Matrix, and Ionization

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14
Q

What are the unexcited trace metals being measured in AAS?

A

Ca and Mg

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15
Q

What is the reason why AAS is better than FEP?

A

More sensitive, accurate, precise, and specific.

Just more sensitive is enough.

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16
Q

What kind of internal SD is used for AAS?

A

AAS has no internal SD.

17
Q

This is used to convert ions to atoms in AAS.

A

Atomizer

18
Q

This is used to modulate the light source in AAS.

A

Chopper

19
Q

What is added to samples in order to form stable complexes with phosphates?

A

Lanthanum or Strontium Chloride

20
Q

For what purpose does Lanthanum and Strontium Chloride serve when it forms complexes with a phosphate?

A

In order to avoid calcium interferences.

Clinical Chemistry [Lecture] (14 decks)

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