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Clinical Chemistry [Lecture] > ANALYTICAL METHODS [ELECTROPHORESIS] > Flashcards

ANALYTICAL METHODS [ELECTROPHORESIS] Flashcards

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1
Q

This method utilizes the migration of charged particles in an electric field.

A

Electrophoresis

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2
Q

Electrophoresis separates protein on the basis of (?).

A

Electric Charge Densities

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3
Q

In electrophoresis, what is the charge of a protein and where does it move towards to?

A

(-) charged, moves towards the anode.

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4
Q

This is the migration of small charged ions.

This method is under electrophoresis.

A

Ionophoresis

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5
Q

This is the migration of charges macromolecules.

This method is under electrophoresis.

ZE

A

Zone Electrophoresis

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6
Q

This is defined as a molecule whose net charge can either be positive or negative depending on the pH.

A

Amphoteric

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7
Q

What is the movement of buffer ions and solvent relative to the fixed support?

A

Electroendosmosis/Endosmosis

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8
Q

What is defined as zero net charge?

A

Isolectric Point

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9
Q

These are the components of electrophoresis.

A
  • Electrical Power
  • Support Medium
  • Buffer
  • Sample
  • Detecting System
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10
Q

What are the examples of support mediums used in electrophoresis?

CA, AG, PG

A
  • Celluloe Acetate
  • Agaroe Gel
  • Polyacrylamide Gel (PAGE)
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11
Q

What are the examples of buffer solutions used in electrophoresis?

B, T

A
  • Barbital (Standard pH)
  • Trisboric EDTA
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12
Q

What are the factors affecting rate of migration?

(+/-), S, S, N, T

A
  • Charge of Molecule
  • Size and Shape
  • Electric Field Strength
  • Nature of Medium
  • Temperature
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13
Q

These are the supporting media for eletrophoresis.

A
  • Paper
  • Starch Gel
  • Cellulose Acetate
  • Agarose Gel
  • Polyacrylamide
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14
Q

What supporting media separates by surface and molecular size?

A

Starch Gel

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15
Q

What supporting media ONLY separates by molecular size?

A

Cellulose Acetate

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16
Q

What supporting media is neutral, separates by electrical charge, and does not bind to protein?

A

Agarose Gel

17
Q

This supporting media is neutral, separates on the basis of charge and molecular size.

A

Polyacrylamide Gel

18
Q

In a polyacrylamide gel media, how many fractions does proteins separate into?

A

20

19
Q

What are the stains used for the visualization of fractions?

A
  • Amido Black
  • Sudan Black
  • Oil Red O
  • Fat Red 7B
  • Coomassie Blue
  • Ponceau S
  • Gold/Silver Stain
20
Q

This is the measure of absorbance of stains through the concentration of dye and protein fraction.

A

Densitometry

21
Q

This method scans and quantitae electrophoretic patterns by reading gel and cellulose acetate membranes.

A

Densitometry

Clinical Chemistry [Lecture] (14 decks)

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