ANALYTIC METHODS [CHROMATOGRAPHY]

Question 1

This method involves the separation of soluble components in a solution by specific differences in physical-chemical charactersitics of the different constitiuents.

Answer 1

Chromatography

Question 2

What are the two forms of chromatography?

Answer 2

• Planar
• Column

Question 3

What are the types of planar chromatography?

Answer 3

• Paper Chromatography
• Thin Layer Chromatography (TLC)

Question 4

What are the types of column chromatography?

Answer 4

• Gas Chromatography
• Liquid Chromatography

Question 5

This is the fractation of sugar and amino acid.

Answer 5

Paper Chromatography

Question 6

What is the sorbent used in paper chromatography?

Answer 6

Whatman Paper

Question 7

This is used for drug screening where the components are identified by comparison with the standards on the same plate.

Answer 7

Thin Layer Chromatography

Question 8

What are the possible biological samples tested in thin layer chromatography?

Answer 8

• Blood
• Urine
• Gastric Fluids

Question 9

What is the sorbent used in thin layer chromatography?

Answer 9

Thin plastic plates impregnated with layer of silica gel or alumina.

Question 10

Extraction of drugs are (?) and pH must be adjusted to reduce the solubility of the drug in (?) phase.

Answer 10

pH dependent and in aqueous phase.

Question 11

Thin layer chromatography is immersed in volatile fluids such as (?).

Answer 11

Acid or Alcohol

Question 12

This kind chromatography is used for the separation of steroids, barbiturates, blood, alcohol, and lipids.

Answer 12

Gas Chromatography

Question 13

This is useful for naturally volatile compounds or can be easily converted into a volatile form.

Answer 13

Gas Chromatography

Question 14

Samples are introduced into the GC column using (?) or a (?).

Answer 14

Hypodermic Syringe or Automated Sampler

Question 15

What is the detector used for GLC?

Answer 15

Flame Ionization

Question 16

What are the gases used in the mobile phase of gas chromatography?

Answer 16

Inert Gases:
- Nitrogen
- Helium
- Hydrogen
- Argon

Question 17

This GC type is used in the differences in absorption at the solid phase surfaces.

Answer 17

Gas Solid Chromatography (GSC)

Question 18

This GC type is when separation occurs by differences in solute partitioning between the gaseous mobile phase and liquid stationary phase.

Answer 18

Gas Liquid Chromatography

Question 19

These are the two phases that is partitioned during GLC.

Answer 19

Gaseous Mobile Phase and Liquid Stationary Phase

Question 20

This method is based on the fragmentation and ionization of molecules using a suitable energy source.

Answer 20

Mass Sepctrometry (MS)

Question 21

Must be separated by (?) before a compound can be detected and quantified by (?)

Answer 21

GC, MS

Question 22

This method can also detect structural information and determination of molecular weight?

Answer 22

Mass Spectrometry

Question 23

This is the gold standard for drug testing.

Answer 23

GC-MS

Question 24

This method can detect 20 inborn errors of metabolism from a single blood spot.

Answer 24

Tanden Mass Spectroscopy (MS/MS)