ANALYTICAL METHODS [FLUOREMETRY OR MOLECULAR LUMINESCENCE SPECTROPHOTOMETRY]

Question 1

This is the other term for fluoremetry.

Answer 1

Molecular Lumincesence Spectrophotometry

Question 2

This measures the amount of light intensity present over a zero background.

Answer 2

Fluoremetry

Question 3

This principle of fluoremetry is the amount of (?) after the (?) by electromagnetic radiation.

Answer 3

Amount of light emitted by a molecule, excitation.

Question 4

These are the two light sources used.

Answer 4

• Mercury Arc
• Xenon Lamp

Question 5

These are the two light detectors used.

Answer 5

• PM Tube
• Phototube

Question 6

Fluoremetry makes use of how many monochromators?

Answer 6

2

Question 7

This monochromator selects the wavelentgh that is best absorbed by the solution to be measures.

Answer 7

Primary Filter

Question 8

This monochromator prevents the incident light in striking the photodetector.

Answer 8

Secondary Filter

Question 9

A fluoremeter is (?) more sensitive that a spectrophotometer.

Answer 9

1000x

Question 10

The fluoremetry process is affected by “(?)”.

Answer 10

Quenching

Question 11

These are the uses for fluoremetry.

Answer 11

Measurements of:
- Porphyrins
- Magnesium
- Calcium
- Catecholamines