Anaerobic/Aerobic Glycolysis (Section 2)

Question 1

Anaerobic Glycolysis (Rate-limiting enzyme? Number of ATP produced? Two phases? Fate of Pyruvate? Enzyme catalyzing reaction? Cellular location?

Answer 1

Rate-limiting enzyme: phosphofructokinase
Number of ATP produced: 2 ATP per 1 glucose molecule
Two phases: Investment and Generation
Fate of Pyruvate: converted into lactic acid
Enzyme catalyzing reaction: Lactate dehydrogenase
Cellular location: Cytoplasm

Question 2

Investment Phase

Answer 2

use 2 ATP to breakdown glucose into 2 P-C-C-C compounds (PGAL; phosphoglyceraldehyde)

Question 3

Generation Phase

Answer 3

each PGAL is converted into pyruvic acid, producing 2 ATP and 1 NADH each

Question 4

Aerobic Glycolysis (Start? Fate of Pyruvate? Enzyme making Pyruvate? Where does Acetyl-CoA go?)

Answer 4

Starts? Cytoplasm (cystol)
Fate of Pyruvate? converted into Acetyl-CoA
Enzyme making Pyruvate? Pyruvate dehydrogenase (PDH) (carboxyl group removed followed by oxidation)
Where does Acetyl-CoA go? to the Krebs cycle in the mitochondria matrix by coenzyme-A (CoA)

Question 5

ATP yielded from Aerobic Glycolysis?

Answer 5

Glycolysis = 2-3
Krebs cycle = 2
ETC = 25 (10 NADH) and 3 (2 FADH)
Total = 32-33 ATP

Question 6

Where do ATP from Aerobic Glycolysis come from?

Answer 6

Glycolysis = Pyruvate (1), Phosphoglycerate Kinase (1)
Krebs cycle = Succinyl-CoA (1)
ETC = oxidative phosphorylation